Chemical Induction of Aminoglycoside Uptake Overcomes Antibiotic Tolerance and Resistance in Staphylococcus aureus [0.03%]
化学诱导的氨基糖苷类药物摄取可克服金黄色葡萄球菌中的抗生素耐受性和抗性
Lauren C Radlinski,Sarah E Rowe,Robert Brzozowski et al.
Lauren C Radlinski et al.
Aminoglycoside antibiotics require proton motive force (PMF) for bacterial internalization. In non-respiring populations, PMF drops below the level required for drug influx, limiting the utility of aminoglycosides against strict and faculta...
A Genome-wide Functional Signature Ontology Map and Applications to Natural Product Mechanism of Action Discovery [0.03%]
全基因组功能特征本体映射及其在天然产物作用机制发现中的应用
Elizabeth A McMillan,Gino Kwon,Jean R Clemenceau et al.
Elizabeth A McMillan et al.
Gene expression signature-based inference of functional connectivity within and between genetic perturbations, chemical perturbations, and disease status can lead to the development of actionable hypotheses for gene function, chemical modes...
Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy [0.03%]
基于相关光镜和电子显微镜的细胞内蛋白质相互作用空间检测 Split-miniSOG技术
Daniela Boassa,Sakina P Lemieux,Varda Lev-Ram et al.
Daniela Boassa et al.
A protein-fragment complementation assay (PCA) for detecting and localizing intracellular protein-protein interactions (PPIs) was built by bisection of miniSOG, a fluorescent flavoprotein derived from the light, oxygen, voltage (LOV)-2 doma...
Mutant-Specific Targeting of Ras G12C Activity by Covalently Reacting Small Molecules [0.03%]
小分子共价结合特异性靶向Ras G12C活性
Roger S Goody,Matthias P Müller,Daniel Rauh
Roger S Goody
In this review we discuss and compare recently introduced molecules that are able to react covalently with an oncogenic mutant of KRas, KRas G12C. Two different classes of compounds in question have been developed, both leading to the mutan...
Bilirubin Links Heme Metabolism to Neuroprotection by Scavenging Superoxide [0.03%]
胆红素通过清除超氧阴离子将血红素代谢与神经保护联系起来
Chirag Vasavda,Ruchita Kothari,Adarsha P Malla et al.
Chirag Vasavda et al.
Bilirubin is one of the most frequently measured metabolites in medicine, yet its physiologic roles remain unclear. Bilirubin can act as an antioxidant in vitro, but whether its redox activity is physiologically relevant is unclear because ...
Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases [0.03%]
工程光活化腺苷酸环化酶隔室生成cAMP
Colin P O&#x;Banion,Brianna M Vickerman,Lauren Haar et al.
Colin P O&#x;Banion et al.
Because small-molecule activators of adenylyl cyclases (AC) affect ACs cell-wide, it is challenging to explore the signaling consequences of AC activity emanating from specific intracellular compartments. We explored this issue using a seri...
Activation of Caspase-6 Is Promoted by a Mutant Huntingtin Fragment and Blocked by an Allosteric Inhibitor Compound [0.03%]
突变型亨廷顿蛋白片段通过激活半胱天冬酶-6导致神经元死亡且其活性被异构调节抑制剂阻断
Dagmar E Ehrnhoefer,Niels H Skotte,Jeanette Reinshagen et al.
Dagmar E Ehrnhoefer et al.
Aberrant activation of caspase-6 (C6) in the absence of other hallmarks of apoptosis has been demonstrated in cells and tissues from patients with Huntington disease (HD) and animal models. C6 activity correlates with disease progression in...
Evert Njomen,Jetze J Tepe
Evert Njomen
The proteolytic arm of the protein homeostasis network is maintained by both the ubiquitin-proteasome system (UPS) and autophagy. A well-balanced crosstalk between the two catabolic pathways ensures energy-efficient maintenance of cellular ...
Jae Won Chang,Raymond E Moellering
Jae Won Chang
In this issue of Cell Chemical Biology, Cook et al. (2019) report a new small-molecule activator that enhances osteogenesis and skeletal regeneration in developmental and adult animal models, respectively. This discovery has therapeutic pot...
Interactome Changes Quantified to Identify the ER Proteostasis Network to Fight Amyloid Diseases [0.03%]
量化互作组的变化以识别内质网蛋白质稳态网络对抗淀粉样疾病
Ya-Juan Wang,Ting-Wei Mu
Ya-Juan Wang
In this issue of Cell Chemical Biology, Plate et al. (2019) used quantitative interactome proteomics to define the molecular mechanism by which ATF6 activation reduces amyloidogenic protein secretion. These results shed light on preventing ...