In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling [0.03%]
O-岩藻糖修饰LRP6使其内吞并抑制Wnt/β-catenin信号通路
Senlian Hong,Lei Feng,Yi Yang et al.
Senlian Hong et al.
Wnt/β-catenin signaling regulates critical, context-dependent transcription in numerous physiological events. Among the well-documented mechanisms affecting Wnt/β-catenin activity, modification of N-glycans by L-fucose is the newest and t...
Pharmacological Targeting of Vacuolar H+-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer [0.03%]
靶向作用于V1G亚基的 vacuolar H+-ATP酶治疗多药耐药癌症
Yuezhou Wang,Lei Zhang,Yanling Wei et al.
Yuezhou Wang et al.
Multidrug resistance (MDR) in cancer remains a major challenge for the success of chemotherapy. Natural products have been a rich source for the discovery of drugs against MDR cancers. Here, we applied high-throughput cytotoxicity screening...
Macrocyclic Peptide-Mediated Blockade of the CD47-SIRPα Interaction as a Potential Cancer Immunotherapy [0.03%]
环肽介导的CD47-SIRPα相互作用阻断作为癌症免疫治疗的潜力
Daisuke Hazama,Yizhen Yin,Yoji Murata et al.
Daisuke Hazama et al.
Medium-sized macrocyclic peptides are an alternative to small compounds and large biomolecules as a class of pharmaceutics. The CD47-SIRPα signaling axis functions as an innate immune checkpoint that inhibits phagocytosis in phagocytes and...
Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells [0.03%]
点亮RNA生物学：在活哺乳动物细胞中成像RNA的技术
Esther Braselmann,Colin Rathbun,Erin M Richards et al.
Esther Braselmann et al.
The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the main building blocks of the cell. But this over simplified linear transmission of information overlooks the vast majority of the genome produces RNAs that do...
A Systematic Analysis of Mosquito-Microbiome Biosynthetic Gene Clusters Reveals Antimalarial Siderophores that Reduce Mosquito Reproduction Capacity [0.03%]
蚊子微生物群生物合成基因簇的系统分析揭示了抗疟原虫铁载体可减少蚊子繁殖能力
Jack G Ganley,Ashmita Pandey,Kayla Sylvester et al.
Jack G Ganley et al.
Advances in infectious disease control strategies through genetic manipulation of insect microbiomes have heightened interest in microbially produced small molecules within mosquitoes. Herein, 33 mosquito-associated bacterial genomes were m...
Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides [0.03%]
野生型糖激酶底物普适性研究及多种氟化单糖的 profiling
Tessa Keenan,Fabio Parmeggiani,Julien Malassis et al.
Tessa Keenan et al.
Fluorinated sugar-1-phosphates are of emerging importance as intermediates in the chemical and biocatalytic synthesis of modified oligosaccharides, as well as probes for chemical biology. Here we present a systematic study of the activity o...
Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies [0.03%]
利用NanoBiT连接的纳米体监测CXCR4上的别构相互作用
Mark Soave,Raimond Heukers,Barrie Kellam et al.
Mark Soave et al.
Camelid single-domain antibody fragments (nanobodies) offer the specificity of an antibody in a single 15-kDa immunoglobulin domain. Their small size allows for easy genetic manipulation of the nanobody sequence to incorporate protein tags,...
Akul Y Mehta,Ravi Kumar H Veeraiah,Sucharita Dutta et al.
Akul Y Mehta et al.
Glycan recognition is typically studied using free glycans, but glycopeptide presentations represent more physiological conditions for glycoproteins. To facilitate studies of glycopeptide recognition, we developed Glyco-SPOT synthesis, whic...
Specific Knockdown of α-Synuclein by Peptide-Directed Proteasome Degradation Rescued Its Associated Neurotoxicity [0.03%]
特定敲除α-突触核蛋白的肽定向蛋白酶体降解可抢救其相关神经毒性
Jing Qu,Xiaoxi Ren,Fenqin Xue et al.
Jing Qu et al.
Published Erratum
Cell chemical biology. 2020 Jun 18;27(6):763. DOI:10.1016/j.chembiol.2020.05.012 2020
Mizuho Horioka,Thomas Huber,Thomas P Sakmar
Mizuho Horioka
In this issue of Cell Chemical Biology, White et al. (2020) describe CRISPR/Cas9-mediated tagging of GPCRs and β-arrestin to provide a method to study receptor signaling in cells under conditions of endogenous genetic control. The strategy...