A biaryl-linked tripeptide from Planomonospora reveals a widespread class of minimal RiPP gene clusters [0.03%]
一种双芳基连接的三肽及其揭示的一类常见的最小核糖体蛋白合成基因簇来源于Planomonospora物种中
Mitja M Zdouc,Mohammad M Alanjary,Guadalupe S Zarazúa et al.
Mitja M Zdouc et al.
Microbial natural products impress by their bioactivity, structural diversity, and ingenious biosynthesis. While screening the less exploited actinobacterial genus Planomonospora, two cyclopeptides were discovered, featuring an unusual Tyr-...
Dual targeting of DDX3 and eIF4A by the translation inhibitor rocaglamide A [0.03%]
Rocaglamide A抑制翻译中的DDX3和eIF4A的双重靶向作用
Mingming Chen,Miwako Asanuma,Mari Takahashi et al.
Mingming Chen et al.
The translation inhibitor rocaglamide A (RocA) has shown promising antitumor activity because it uniquely clamps eukaryotic initiation factor (eIF) 4A onto polypurine RNA for selective translational repression. As eIF4A has been speculated ...
A Robust, GFP-Orthogonal Photoswitchable Inhibitor Scaffold Extends Optical Control over the Microtubule Cytoskeleton [0.03%]
一种稳健的、GFP正交的光控抑制剂骨架扩展了对微管细胞骨架的光学控制范围
Li Gao,Joyce C M Meiring,Yvonne Kraus et al.
Li Gao et al.
Optically controlled chemical reagents, termed "photopharmaceuticals," are powerful tools for precise spatiotemporal control of proteins particularly when genetic methods, such as knockouts or optogenetics are not viable options. However, c...
Hsin-Mei Huang,Philipp Stephan,Hajo Kries
Hsin-Mei Huang
Diffusive escape of intermediates limits the rate enhancement that nanocontainers or macromolecular scaffolds can provide for artificial biocatalytic cascades. Nonribosomal peptide synthetases (NRPSs) naturally form gigantic assembly lines ...
Sylvia L Rivera,Akbar Espaillat,Arjun K Aditham et al.
Sylvia L Rivera et al.
Transpeptidation reinforces the structure of cell-wall peptidoglycan, an extracellular heteropolymer that protects bacteria from osmotic lysis. The clinical success of transpeptidase-inhibiting β-lactam antibiotics illustrates the essentia...
K27-Linked Diubiquitin Inhibits UCHL3 via an Unusual Kinetic Trap [0.03%]
一种通过不寻常的动态陷阱抑制UCHL3的K27连接二泛素
Gabriëlle B A van Tilburg,Andrea G Murachelli,Alexander Fish et al.
Gabriëlle B A van Tilburg et al.
Functional analysis of lysine 27-linked ubiquitin chains (K27Ub) is difficult due to the inability to make them through enzymatic methods and due to a lack of model tools and substrates. Here we generate a series of ubiquitin (Ub) tools to ...
Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary [0.03%]
小鼠脑和垂体内遗传定义的细胞类型的神经肽组分析
Lloyd D Fricker,Alexandre K Tashima,Amanda K Fakira et al.
Lloyd D Fricker et al.
Neuropeptides and peptide hormones are important cell-cell signaling molecules that mediate many physiological processes. Unlike classic neurotransmitters, peptides undergo cell-type-specific post-translational modifications that affect the...
Systematic Activity Maturation of a Single-Domain Antibody with Non-canonical Amino Acids through Chemical Mutagenesis [0.03%]
基于非天然氨基酸的化学诱变单域抗体系统发育成熟方法
Philip R Lindstedt,Francesco A Aprile,Pietro Sormanni et al.
Philip R Lindstedt et al.
Great advances have been made over the last four decades in therapeutic and diagnostic applications of antibodies. The activity maturation of antibody candidates, however, remains a significant challenge. To address this problem, we present...
Linna Zhou,Hironori Ishizaki,Michaela Spitzer et al.
Linna Zhou et al.
Published Erratum
Cell chemical biology. 2020 Nov 19;27(11):1452. DOI:10.1016/j.chembiol.2020.10.011 2020
Dual Targeting of v-ATPase and mTORC1 Signaling Disarms Multidrug-Resistant Cancers [0.03%]
v-ATP酶和mTORC1信号的双重靶向清除多药耐药癌症
Tayah Turocy,Jason M Crawford
Tayah Turocy
Chemotherapeutic treatments are frequently impeded by the development of multidrug resistance (MDR). In this issue of Cell Chemical Biology, Wang et al. (2020) identify the natural product verucopeptin as having therapeutic potential toward...