Fatty acid chain length drives lysophosphatidylserine-dependent immunological outputs [0.03%]
脂肪酸链长决定了磷脂酰丝氨酸依赖的免疫反应
Neha Khandelwal,Minhaj Shaikh,Amol Mhetre et al.
Neha Khandelwal et al.
In humans, lysophosphatidylserines (lyso-PSs) are potent lipid regulators of important immunological processes. Given their structural diversity and commercial paucity, here we report the synthesis of methyl esters of lyso-PS (Me-lyso-PSs) ...
Discovery of a σ1 receptor antagonist by combination of unbiased cell painting and thermal proteome profiling [0.03%]
通过无偏见的细胞形态分析和热蛋白组学剖析发现σ1受体拮抗剂
Julian Wilke,Tatsuro Kawamura,Hao Xu et al.
Julian Wilke et al.
Phenotypic screening for bioactive small molecules is typically combined with affinity-based chemical proteomics to uncover the respective molecular targets. However, such assays and the explored bioactivity are biased toward the monitored ...
S1PR3-G12-biased agonist ALESIA targets cancer metabolism and promotes glucose starvation [0.03%]
S1PR3-G12偏向激动剂ALESIA靶向癌症代谢并促进葡萄糖饥饿
Masayasu Toyomoto,Asuka Inoue,Kei Iida et al.
Masayasu Toyomoto et al.
Metabolic activities are altered in cancer cells compared with those in normal cells, and the cancer-specific pathway becomes a potential therapeutic target. Higher cellular glucose consumption, which leads to lower glucose levels, is a hal...
Phosphorylation-regulated HMGA1a-P53 interaction unveils the function of HMGA1a acidic tail phosphorylations via synthetic proteins [0.03%]
通过合成蛋白揭示HMGA1a酸性尾部磷酸化功能的磷酸化调节HMGA1a-P53相互作用
Tongyao Wei,Heng Liu,Bizhu Chu et al.
Tongyao Wei et al.
As a typical member of intrinsically disordered proteins (IDPs), HMGA1a carries many post-translational modifications (PTMs). To study the undefined function of acidic tail phosphorylations, seven HMGA1a proteins with site-specific modifica...
MGST1 is a redox-sensitive repressor of ferroptosis in pancreatic cancer cells [0.03%]
MGST1是胰腺癌细胞铁死亡的氧化还原敏感型抑制因子
Feimei Kuang,Jiao Liu,Yangchun Xie et al.
Feimei Kuang et al.
Ferroptosis is a type of nonapoptotic cell death driven by lipid peroxidation. Here, we show a key role of MGST1 in inhibiting ferroptosis in cell cultures and mouse xenograft models. Ferroptosis activators induce MGST1 upregulation in huma...
Ellen L Berg
Ellen L Berg
Phenotypic drug discovery (PDD) uses biological systems directly for new drug screening. While PDD has proved effective in the discovery of drugs with novel mechanisms, for broader adoption, key challenges need resolution: progression of po...
Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly [0.03%]
peri小胞表达的SpyTag抗体片段能够实现快速模块化组装
Christian Hentrich,Sarah-Jane Kellmann,Mateusz Putyrski et al.
Christian Hentrich et al.
Antibodies are essential tools in research and diagnostics. Although antibody fragments typically obtained from in vitro selection can be rapidly produced in bacteria, the generation of full-length antibodies or the modification of antibodi...
Chemoproteomics-enabled discovery of covalent RNF114-based degraders that mimic natural product function [0.03%]
基于化学蛋白质组学的共价RNF114降解剂的发现，此类降解剂可模拟天然产物的作用
Mai Luo,Jessica N Spradlin,Lydia Boike et al.
Mai Luo et al.
The translation of functionally active natural products into fully synthetic small-molecule mimetics has remained an important process in medicinal chemistry. We recently discovered that the terpene natural product nimbolide can be utilized...
Light-controllable RNA-protein devices for translational regulation of synthetic mRNAs in mammalian cells [0.03%]
可控光的RNA-蛋白质装置在哺乳动物细胞中合成mRNA的翻译调控中的应用
Hideyuki Nakanishi,Tatsuyuki Yoshii,Shunsuke Kawasaki et al.
Hideyuki Nakanishi et al.
The photo-regulation of transgene expression is one effective approach in mammalian synthetic biology due to its high spatial and temporal resolution. While DNAs are mainly used as vectors, modified RNAs (modRNAs) are also useful for medica...
Small-molecule probe reveals a kinase cascade that links stress signaling to TCF/LEF and Wnt responsiveness [0.03%]
小分子化合物筛选发现一条连接压力信号与TCF/LEF和Wnt信号通路的激酶途径
Jiongjia Cheng,Masanao Tsuda,Karl Okolotowicz et al.
Jiongjia Cheng et al.
Wnt signaling plays a central role in tissue maintenance and cancer. Wnt activates downstream genes through β-catenin, which interacts with TCF/LEF transcription factors. A major question is how this signaling is coordinated relative to ti...