Tag you're it: A phosphatase inhibitor changes the fate of intracellular mycobacteria [0.03%]
接力游戏开始：一种磷酸酶抑制剂改变细胞内结核杆菌的命运
Charul Jani,Amy K Barczak
Charul Jani
In this issue of Cell Chemical Biology, Berton and colleagues describe a small-molecule inhibitor of the metal-dependent phosphatase PPM1A that enhances phosphorylation of the autophagy adapter p62. Inhibiting PPM1A results in enhanced clea...
Capsaicin ameliorates inflammation in a TRPV1-independent mechanism by inhibiting PKM2-LDHA-mediated Warburg effect in sepsis [0.03%]
辣椒素通过抑制PKM2-LDHA介导的瓦堡效应独立于TRPV1改善脓毒症炎症
Qian Zhang,Piao Luo,Fei Xia et al.
Qian Zhang et al.
Sepsis is a systemic inflammatory response syndrome with high mortality and morbidity worldwide. In this study, we demonstrate that capsaicin not only suppresses inflammation in lipopolysaccharide (LPS)-induced macrophages, but also effecti...
Allosteric HSP70 inhibitors perturb mitochondrial proteostasis and overcome proteasome inhibitor resistance in multiple myeloma [0.03%]
别构HSP70抑制剂可干扰多发性骨髓瘤的线粒体蛋白质稳态并克服蛋白酶体抑制剂的耐药性
Ian D Ferguson,Yu-Hsiu T Lin,Christine Lam et al.
Ian D Ferguson et al.
Proteasome inhibitor (PI) resistance remains a central challenge in multiple myeloma. To identify pathways mediating resistance, we first mapped proteasome-associated genetic co-dependencies. We identified heat shock protein 70 (HSP70) chap...
Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells [0.03%]
基于PROTAC介导的BCL11A蛋白降解在红细胞中基因去抑制和蛋白质组变化的时空解析
Stuti Mehta,Altantsetseg Buyanbat,Yan Kai et al.
Stuti Mehta et al.
Reactivation of fetal hemoglobin expression by the downregulation of BCL11A is a promising treatment for β-hemoglobinopathies. A detailed understanding of BCL11A-mediated repression of γ-globin gene (HBG1/2) transcription is lacking, as s...
A chemogenetic platform for controlling plasma membrane signaling and synthetic signal oscillation [0.03%]
控制质膜信号和合成信号振荡的化学遗传学平台
Sachio Suzuki,Akinobu Nakamura,Yuka Hatano et al.
Sachio Suzuki et al.
Chemogenetic methods enabling the rapid translocation of specific proteins to the plasma membrane (PM) in a single protein-single ligand manner are useful tools in cell biology. We recently developed a technique, in which proteins fused to ...
A campaign targeting a conserved Hsp70 binding site uncovers how subcellular localization is linked to distinct biological activities [0.03%]
靶向一个保守的Hsp70结合位点开展的一项研究揭示了亚细胞定位与不同生物学功能之间的关联
Hao Shao,Shuhei Taguwa,Luke Gilbert et al.
Hao Shao et al.
The potential of small molecules to localize within subcellular compartments is rarely explored. To probe this question, we measured the localization of Hsp70 inhibitors using fluorescence microscopy. We found that even closely related anal...
Cell-free production of personalized therapeutic phages targeting multidrug-resistant bacteria [0.03%]
无细胞生产针对耐多药细菌的个性化治疗噬菌体
Quirin Emslander,Kilian Vogele,Peter Braun et al.
Quirin Emslander et al.
Bacteriophages are potent therapeutics against biohazardous bacteria, which rapidly develop multidrug resistance. However, routine administration of phage therapy is hampered by a lack of rapid production, safe bioengineering, and detailed ...
Leslie Magtanong,Grace D Mueller,Kevin J Williams et al.
Leslie Magtanong et al.
Ferroptosis is an important mediator of pathophysiological cell death and an emerging target for cancer therapy. Whether ferroptosis sensitivity is governed by a single regulatory mechanism is unclear. Here, based on the integration of 24 p...
Juan Manuel Povedano,Vicky Li,Katherine E Lake et al.
Juan Manuel Povedano et al.
Ewing sarcoma (EWS) is a pediatric malignancy driven by the EWSR1-FLI1 fusion protein formed by the chromosomal translocation t(11; 22). The small molecule TK216 was developed as a first-in-class direct EWSR1-FLI1 inhibitor and is in phase ...
A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection [0.03%]
利用CRISPR-Cas9基因敲除筛选技术鉴定调控顶体蛋白组织在肝脏疟疾感染过程中改变的宿主调节因子CENPJ
Kamalakannan Vijayan,Nadia Arang,Ling Wei et al.
Kamalakannan Vijayan et al.
Prior to initiating symptomatic malaria, a single Plasmodium sporozoite infects a hepatocyte and develops into thousands of merozoites, in part by scavenging host resources, likely delivered by vesicles. Here, we demonstrate that host micro...