Structurally distinct PARP7 inhibitors provide new insights into the function of PARP7 in regulating nucleic acid-sensing and IFN-β signaling [0.03%]
结构不同的PARP7抑制剂为理解PARP7在调控核苷酸感应和IFN-β信号中的功能提供了新的见解
Daniel J Sanderson,Kelsie M Rodriguez,Daniel S Bejan et al.
Daniel J Sanderson et al.
The mono-ADP-ribosyltransferase PARP7 has emerged as a key negative regulator of cytosolic NA-sensors of the innate immune system. We apply a rational design strategy for converting a pan-PARP inhibitor into a potent selective PARP7 inhibit...
Mike Lange,James A Olzmann
Mike Lange
Hydropersulfides are implicated in cellular responses to oxidative stress. In two landmark papers, Barayeu et al. and Wu et al. reveal that endogenous hydropersulfides are hydrogen atom donors that scavenge lipid radicals, thereby preventin...
Real-time non-invasive fluorescence imaging of gut commensal bacteria to detect dynamic changes in the microbiome of live mice [0.03%]
利用实时无创荧光成像技术观察肠道共生物细菌以检测活鼠体内微生物群的动态变化
Alexis J Apostolos,Mahendra D Chordia,Sree H Kolli et al.
Alexis J Apostolos et al.
In mammals, gut commensal microbiota interact extensively with the host, and the same interactions can be dysregulated in diseased states. Animal imaging is a powerful technique that is widely used to diagnose, measure, and track biological...
Jung-Ming G Lin,Savvas Kourtis,Ritobrata Ghose et al.
Jung-Ming G Lin et al.
While it is well known that expression levels of metabolic enzymes regulate the metabolic state of the cell, there is mounting evidence that the converse is also true, that metabolite levels themselves can modulate gene expression via epige...
Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors [0.03%]
基于酞嗪酮的PARP-1抑制剂的DNA结合及靶点驻留时间的别构调控驱动了其细胞毒性作用
Moriah R Arnold,Marie-France Langelier,Jessica Gartrell et al.
Moriah R Arnold et al.
Allosteric coupling between the DNA binding site to the NAD+-binding pocket drives PARP-1 activation. This allosteric communication occurs in the reverse direction such that NAD+ mimetics can enhance PARP-1's affinity for DNA, referred to a...
A photocaged orexin-B for spatiotemporally precise control of orexin signaling [0.03%]
用于精确空间和时间控制催产素信号传导的光笼催产素B
Loïc Duffet,Petr V Tatarskiy,Masaya Harada et al.
Loïc Duffet et al.
Orexin neuropeptides carry out important neuromodulatory functions in the brain, yet tools to precisely control the activation of endogenous orexin signaling are lacking. Here, we developed a photocaged orexin-B (photo-OXB) through a C-term...
Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation [0.03%]
全球定位识别EDC3上一个应激响应的酪氨酸位点，调控生物分子凝聚体的形成
Anthony M Ciancone,Seyyedmohsen Hosseinibarkooie,Dina L Bai et al.
Anthony M Ciancone et al.
RNA granules are cytoplasmic condensates that organize biochemical and signaling complexes in response to cellular stress. Functional proteomic investigations under RNA-granule-inducing conditions are needed to identify protein sites involv...
Hengrui Liu,Farhad Forouhar,Annie J Lin et al.
Hengrui Liu et al.
Encouraged by the dependence of drug-resistant, metastatic cancers on GPX4, we examined biophysical mechanisms of GPX4 inhibition, which revealed an unexpected allosteric site. We found that this site was involved in native regeneration of ...
Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions [0.03%]
肽聚糖NlpC/P60肽酶在细菌生理学和宿主相互作用中的作用
Matthew E Griffin,Steven Klupt,Juliel Espinosa et al.
Matthew E Griffin et al.
The bacterial cell wall is composed of a highly crosslinked matrix of glycopeptide polymers known as peptidoglycan that dictates bacterial cell morphology and protects against environmental stresses. Regulation of peptidoglycan turnover is ...
Tianlu Wang,Yuepeng Ke,Yubin Zhou
Tianlu Wang
In this issue of Cell Chemical Biology, Ueda et al. (2022) developed PPAP2 as an improved optogenetic tool to photo-induce PI3K signaling hyperactivation in cancer cells. They demonstrated that enhanced translation of tumor necrosis factor ...