Site-specific dual encoding and labeling of proteins via genetic code expansion [0.03%]
基于遗传密码扩展的蛋白质位点特异性双重编码和标记
Riley M Bednar,P Andrew Karplus,Ryan A Mehl
Riley M Bednar
The ability to selectively modify proteins at two or more defined locations opens new avenues for manipulating, engineering, and studying living systems. As a chemical biology tool for the site-specific encoding of non-canonical amino acids...
Mitigating the risk of antimalarial resistance via covalent dual-subunit inhibition of the Plasmodium proteasome [0.03%]
通过共价双亚基抑制来降低抗疟疾药物耐药性的风险：针对疟原虫蛋白酶体
Ioanna Deni,Barbara H Stokes,Kurt E Ward et al.
Ioanna Deni et al.
The Plasmodium falciparum proteasome constitutes a promising antimalarial target, with multiple chemotypes potently and selectively inhibiting parasite proliferation and synergizing with the first-line artemisinin drugs, including against a...
Temsavir blocks the immunomodulatory activities of HIV-1 soluble gp120 [0.03%]
Temsavir阻断HIV-1可溶性gp120的免疫调节作用
Jonathan Richard,Jérémie Prévost,Catherine Bourassa et al.
Jonathan Richard et al.
While HIV-1-mediated CD4 downregulation protects infected cells from antibody-dependent cellular cytotoxicity (ADCC), shed gp120 binds to CD4 on uninfected bystander CD4+ T cells, sensitizing them to ADCC mediated by HIV+ plasma. Soluble gp...
Monitoring autophagic flux in vivo revealed its physiological response and significance of heterogeneity in pancreatic beta cells [0.03%]
体内监测自噬流揭示了胰岛β细胞的生理反应及异质性的意义
Shuhei Aoyama,Yuya Nishida,Hirotsugu Uzawa et al.
Shuhei Aoyama et al.
Autophagy plays an essential role in preserving cellular homeostasis in pancreatic beta cells. However, the extent of autophagic flux in pancreatic islets induced in various physiological settings remains unclear. In this study, we generate...
Getting in on the action: New tools to see SARS-CoV-2 infect a cell [0.03%]
行动之中：观察SARS-CoV-2感染细胞的新工具
Scott B Hansen,Zixuan Yuan
Scott B Hansen
In this issue of Cell Chemical Biology, Miao et al. develop probes for live cell tracking of SARS-CoV-2. The probes reveal the endocytic pathway for viral entry. Unexpectedly, the antiviral compound BafA1 traps the virus on the cell surface...
Eric S Wang,Nathanael S Gray
Eric S Wang
In this issue of Cell Chemical Biology, Bonazzi et al. demonstrate that pharmacologically degrading the transcription factor Helios (IKZF2) results in destabilization of regulatory T cells, which normally restrain anti-tumor immunity. These...
Chemoproteomics-enabled discovery of a covalent molecular glue degrader targeting NF-κB [0.03%]
基于化学蛋白质组学的共价分子胶降解剂研究及靶向NF-κB的应用
Elizabeth A King,Yoojin Cho,Nathan S Hsu et al.
Elizabeth A King et al.
Targeted protein degradation has arisen as a powerful therapeutic modality for degrading disease targets. While proteolysis-targeting chimera (PROTAC) design is more modular, the discovery of molecular glue degraders has been more challengi...
Ainsliadimer A induces ROS-mediated apoptosis in colorectal cancer cells via directly targeting peroxiredoxin 1 and 2 [0.03%]
阿斯利二聚体A通过直接靶向过氧化还原蛋白1和2介导的ROS诱导结直肠癌细胞凋亡
Chao Lv,Yun Huang,Qun Wang et al.
Chao Lv et al.
The peroxiredoxin (PRDX) family is a class of antioxidant enzymes with peroxidase activity. Human PRDXs currently have six members (PRDX1-6), which are gradually becoming potential therapeutic targets for major diseases such as cancer. In t...
Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein [0.03%]
化合物RMY-205治疗SARS-CoV-2核衣壳蛋白诱导的肺纤维化的疗效研究
Zhi-Yuan Zhang,Cui-Yu Ju,Liu-Zheng Wu et al.
Zhi-Yuan Zhang et al.
Pulmonary fibrosis is a typical sequela of coronavirus disease 2019 (COVID-19), which is linked with a poor prognosis for COVID-19 patients. However, the underlying mechanism of pulmonary fibrosis induced by severe acute respiratory syndrom...
Redox proteomics combined with proximity labeling enables monitoring of localized cysteine oxidation in cells [0.03%]
基于红氧电位的蛋白质组学结合亲邻标记技术可监测细胞内半胱氨酸局部氧化状态
Eleni A Kisty,Julia A Falco,Eranthie Weerapana
Eleni A Kisty
Reactive oxygen species (ROS) can modulate protein function through cysteine oxidation. Identifying protein targets of ROS can provide insight into uncharacterized ROS-regulated pathways. Several redox-proteomic workflows, such as oxidative...