Inhibition of nucleolar transcription by oxaliplatin involves ATM/ATR kinase signaling [0.03%]
奥沙利铂通过ATM/ATR激酶信号抑制核仁转录
Misha Nechay,Danyang Wang,Ralph E Kleiner
Misha Nechay
Platinum (Pt) compounds are an important class of anti-cancer therapeutics, but outstanding questions remain regarding their mechanism of action. Here, we demonstrate that oxaliplatin, a Pt drug used to treat colorectal cancer, inhibits rRN...
Integration of metabolism and regulation reveals rapid adaptability to growth on non-native substrates [0.03%]
代谢和调控的结合揭示了快速适应生长的非天然基质的能力
Vikas D Trivedi,Sean F Sullivan,Debika Choudhury et al.
Vikas D Trivedi et al.
Engineering synthetic heterotrophy is a key to the efficient bio-based valorization of renewable and waste substrates. Among these, engineering hemicellulosic pentose utilization has been well-explored in Saccharomyces cerevisiae (yeast) ov...
Proximity-labeling chemoproteomics defines the subcellular cysteinome and inflammation-responsive mitochondrial redoxome [0.03%]
邻近标记化学蛋白质组学定义了亚细胞半胱蛋白组图和炎性反应线粒体氧化还原图
Tianyang Yan,Ashley R Julio,Miranda Villanueva et al.
Tianyang Yan et al.
Proteinaceous cysteines function as essential sensors of cellular redox state. Consequently, defining the cysteine redoxome is a key challenge for functional proteomic studies. While proteome-wide inventories of cysteine oxidation state are...
Novel Frizzled-specific antibody-based Wnt mimetics and Wnt superagonists selectively activate WNT/β-catenin signaling in target tissues [0.03%]
新型的Frizzled特异性抗体类Wnt激动剂及其超级激动剂选择性地激活目标组织中的Wnt/β-连环蛋白信号通路
Yorick Post,Archana Dilip,Liqin Xie et al.
Yorick Post et al.
WNTs are essential factors for stem cell biology, embryonic development, and for maintaining homeostasis and tissue repair in adults. Difficulties in purifying WNTs and their lack of receptor selectivity have hampered research and regenerat...
Utilizing directed evolution to interrogate and optimize CRISPR/Cas guide RNA scaffolds [0.03%]
运用定向进化技术研究并优化CRISPR/Cas引导RNA支架结构
Korie Bush,Giulia I Corsi,Amy C Yan et al.
Korie Bush et al.
CRISPR-based editing has revolutionized genome engineering despite the observation that many DNA sequences remain challenging to target. Unproductive interactions formed between the single guide RNA's (sgRNA) Cas9-binding scaffold domain an...
Morphological subprofile analysis for bioactivity annotation of small molecules [0.03%]
小分子生物活性注释的形态亚型分析
Axel Pahl,Beate Schölermann,Philipp Lampe et al.
Axel Pahl et al.
Fast prediction of the mode of action (MoA) for bioactive compounds would immensely foster bioactivity annotation in compound collections and may early on reveal off-targets in chemical biology research and drug discovery. Morphological pro...
Chemical genomics reveals targetable programs of human cancers rooted in pluripotency [0.03%]
化学基因组学揭示了多能性根植的人类癌症可靶向程序
Luca Orlando,Yannick D Benoit,Jennifer C Reid et al.
Luca Orlando et al.
Overlapping principles of embryonic and tumor biology have been described, with recent multi-omics campaigns uncovering shared molecular profiles between human pluripotent stem cells (hPSCs) and adult tumors. Here, using a chemical genomic ...
Identification of triazenyl indoles as inhibitors of fungal fatty acid biosynthesis with broad-spectrum activity [0.03%]
三氮杂吲哚类抗真菌化合物的设计、合成及评价其抑制脂肪酸生物合成的广谱抗菌活性研究
Kali R Iyer,Sheena C Li,Nicole M Revie et al.
Kali R Iyer et al.
Rising drug resistance among pathogenic fungi, paired with a limited antifungal arsenal, poses an increasing threat to human health. To identify antifungal compounds, we screened the RIKEN natural product depository against representative i...
NREP contributes to development of NAFLD by regulating one-carbon metabolism in primary human hepatocytes [0.03%]
NREP通过调节人原代肝细胞的一碳代谢促进NAFLD的发展
Dario F De Jesus,Tomohiko Kimura,Manoj K Gupta et al.
Dario F De Jesus et al.
Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease. We recently discovered that neuronal regeneration-related protein (NREP/P311), an epigenetically regulated gene reprogrammed by parental metabolic ...
Spike-protein proteolytic antibodies in COVID-19 convalescent plasma contribute to SARS-CoV-2 neutralization [0.03%]
新冠恢复期血浆中的刺突蛋白蛋白酶抗体有助于中和SARS-CoV-2
Scott A McConnell,Jaiprasath Sachithanandham,Nathan J Mudrak et al.
Scott A McConnell et al.
Understanding the mechanisms of antibody-mediated neutralization of SARS-CoV-2 is critical in combating the COVID-19 pandemic. Based on previous reports of antibody catalysis, we investigated the proteolysis of spike (S) by antibodies in CO...