Highly efficient cellular expression of circular mRNA enables prolonged protein expression [0.03%]
高效的细胞表达环形mRNA以实现长时间的蛋白质表达
Mildred J Unti,Samie R Jaffrey
Mildred J Unti
A major problem with mRNA therapeutics is that mRNA is usually degraded within a few hours after entering the cytosol. New approaches for in vitro synthesis of circular mRNA have allowed increased levels and duration of protein synthesis fr...
Methotrexate-based PROTACs as DHFR-specific chemical probes [0.03%]
基于甲氨蝶呤的PROTAC类小分子化合物作为DHFR特异性化学探针
Sandeep Rana,Patricia Dranchak,Jayme L Dahlin et al.
Sandeep Rana et al.
Methotrexate (MTX) is a tight-binding dihydrofolate reductase (DHFR) inhibitor, used as both an antineoplastic and immunosuppressant therapeutic. MTX, like folate undergoes folylpolyglutamate synthetase-mediated γ-glutamylation, which affe...
Guangdong Yang
Guangdong Yang
Protein quality control is often disrupted in heart diseases. In this issue of Cell Chemical Biology, Yang et al.1 describe the selective E3 ligase SPOP as a pro-fibrotic factor for inducing cardiac fibrosis through RACK1 degradation-mediat...
A new chink in cancer's armor: Unleashing cell death by selective PP5 inhibition [0.03%]
抗癌新策略：通过选择性抑制PP5蛋白诱导癌细胞凋亡
Patricija van Oosten-Hawle
Patricija van Oosten-Hawle
Serine/threonine protein phosphatases play a significant role in the survival and propagation of multiple cancers. In this issue of Cell Chemical Biology, Ahanin et al.1 demonstrate that protein phosphatase 5 (PP5) dephosphorylates and inac...
HATS off to KAT6A/B inhibitors: A new way to target estrogen-receptor-positive breast cancer [0.03%]
脱帽致敬KAT6A/B抑制剂：一种新的针对雌激素受体阳性乳腺癌的治疗方法
Rinath Jeselsohn,Kornelia Polyak
Rinath Jeselsohn
Inhibitors for the KAT6 family of histone acetyltransferases (HATs) have been actively pursued due to the oncogenic roles of KAT6A in human cancer. CTx-648 is a novel KAT6A/B inhibitor with excellent pharmacokinetic properties and in vivo e...
Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation [0.03%]
组蛋白甲基转移酶G9a在慢性炎症翻译调控中的非经典功能研究
Adil Muneer,Li Wang,Ling Xie et al.
Adil Muneer et al.
We report a novel translation-regulatory function of G9a, a histone methyltransferase and well-understood transcriptional repressor, in promoting hyperinflammation and lymphopenia; two hallmarks of endotoxin tolerance (ET)-associated chroni...
Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays [0.03%]
利用细胞检测手段对NLRP3的直接结合及炎性小体的功能抑制进行验证
Kelly A Teske,Cesear Corona,Jennifer Wilkinson et al.
Kelly A Teske et al.
As a key regulator of the innate immune system, the NLRP3 inflammasome responds to a variety of environmental insults through activation of caspase-1 and release of the proinflammatory cytokines IL-1β and IL-18. Aberrant NLRP3 inflammasome...
Differential network analysis of ROS1 inhibitors reveals lorlatinib polypharmacology through co-targeting PYK2 [0.03%]
ROS1抑制剂的差异网络分析揭示了lorlatinib通过共同靶向PYK2产生多药理效应
Yi Liao,Lily L Remsing Rix,Xueli Li et al.
Yi Liao et al.
Multiple tyrosine kinase inhibitors (TKIs) are often developed for the same indication. However, their relative overall efficacy is frequently incompletely understood and they may harbor unrecognized targets that cooperate with the intended...
Wei Huang,Scott T Laughlin
Wei Huang
In classic bioorthogonal labeling experiments, the cell's biosynthetic machinery incorporates bioorthogonal tags, creating tagged biomolecules that are subsequently reacted with a corresponding bioorthogonal partner. This two-step approach ...
Noncanonical Streptococcus sanguinis ComCDE circuitry integrates environmental cues in transformation outcome decision [0.03%]
非规范的链球菌转化结果决策中的环境线索整合电路系
Mingzhe Guo,Clay P Renshaw,Ryan W Mull et al.
Mingzhe Guo et al.
Natural competence is the principal driver of streptococcal evolution. While acquisition of new traits could facilitate rapid fitness improvement for bacteria, entry into the competent state is a highly orchestrated event, involving an inte...