Host specific sphingomyelin is critical for replication of diverse RNA viruses [0.03%]
宿主特异性鞘磷脂对多种RNA病毒复制至关重要
Shuo Han,Xiaolei Ye,Jintong Yang et al.
Shuo Han et al.
Lipids and lipid metabolism play an important role in RNA virus replication, which typically occurs on host cell endomembrane structures in the cytoplasm through mechanisms that are not yet fully identified. We conducted genome-scale CRISPR...
Chemical tools to expand the ligandable proteome: Diversity-oriented synthesis-based photoreactive stereoprobes [0.03%]
基于多样性导向合成的光反应性立体探针：扩大可结合蛋白组的化学工具
Daisuke Ogasawara,David B Konrad,Zher Yin Tan et al.
Daisuke Ogasawara et al.
Chemical proteomics enables the global analysis of small molecule-protein interactions in native biological systems and has emerged as a versatile approach for ligand discovery. The range of small molecules explored by chemical proteomics h...
A PROTAC degrader suppresses oncogenic functions of PTK6 inducing apoptosis of breast cancer cells [0.03%]
一种蛋白降解剂 PROTAC 可以抑制 PTK6 的致癌功能并诱导乳腺癌细胞发生凋亡
Criseyda Martinez,Yan Xiong,Alison Bartkowski et al.
Criseyda Martinez et al.
Protein tyrosine kinase 6 (PTK6), a non-receptor tyrosine kinase, is an oncogenic driver in many tumor types. However, agents that therapeutically target PTK6 are lacking. Although several PTK6 kinase inhibitors have been developed, none ha...
Degradome analysis to identify direct protein substrates of small-molecule degraders [0.03%]
降解组学分析以识别小分子降解剂的直接蛋白质底物
Marco Jochem,Anna Schrempf,Lina-Marie Wagner et al.
Marco Jochem et al.
Targeted protein degradation (TPD) has emerged as a powerful strategy to selectively eliminate cellular proteins using small-molecule degraders, offering therapeutic promise for targeting proteins that are otherwise undruggable. However, a ...
Alexandria N Van Scoyk,Orlando Antelope,Donald E Ayer et al.
Alexandria N Van Scoyk et al.
Lysine acylation can direct protein function, localization, and interactions. Sirtuins deacylate lysine toward maintaining cellular homeostasis, and their aberrant expression contributes to the pathogenesis of multiple conditions, including...
Mackenzie W Krone,Craig M Crews
Mackenzie W Krone
Targeted protein degradation (TPD) has greatly advanced as a therapeutic strategy in the past two decades, and we are on the cusp of rationally designed protein degraders reaching clinical approval. Offering pharmacological advantages relat...
A LANA peptide inhibits tumor growth by inducing CHD4 protein cleavage and triggers cell death [0.03%]
一种LANA肽通过诱导CHD4蛋白裂解来抑制肿瘤生长并引发细胞死亡
Hiroki Miura,Kang-Hsin Wang,Tomoki Inagaki et al.
Hiroki Miura et al.
Kaposi's sarcoma-associated herpesvirus (KSHV) establishes a latent infection, and viral genes are poised to be transcribed in the latent chromatin. In the poised chromatins, KSHV latency-associated nuclear antigen (LANA) interacts with cel...
Identification of two repurposed drugs targeting GSDMD oligomerization interface I to block pyroptosis [0.03%]
识别出靶向GSDMD寡聚化界面I的两种老药以阻断细胞焦亡
Yingchao Hu,Honghui Li,Xiangyu Zhang et al.
Yingchao Hu et al.
As an executor of pyroptosis, gasdermin D (GSDMD) plays a critical role in inflammatory diseases and cancer. Thus, GSDMD is currently being widely explored as a drug target. Existing inhibitors targeting GSDMD, such as necrosulfonamide, dis...
Dynamic sampling of a surveillance state enables DNA proofreading by Cas9 [0.03%]
对监控状态进行动态采样使Cas9能够进行DNA校对
Viviane S De Paula,Abhinav Dubey,Haribabu Arthanari et al.
Viviane S De Paula et al.
CRISPR-Cas9 has revolutionized genome engineering applications by programming its single-guide RNA, where high specificity is required. However, the precise molecular mechanism underscoring discrimination between on/off-target DNA sequences...
The liquid lectin array detects compositional glycocalyx differences using multivalent DNA-encoded lectins on phage [0.03%]
基于噬菌体的多价DNA编码凝集素液相芯片技术用于检测糖萼差异
Guilherme M Lima,Zeinab Jame-Chenarboo,Mirat Sojitra et al.
Guilherme M Lima et al.
Selective detection of disease-associated changes in the glycocalyx is an emerging field in modern targeted therapies. Detecting minor glycan changes on the cell surface is a challenge exacerbated by the lack of correspondence between cellu...