Diana Gutiérrez,Lucía Fernández,Ana Rodríguez et al.
Diana Gutiérrez et al.
The growing concern about multi-drug resistant pathogenic bacteria has led to a renewed interest in the study of bacteriophages as antimicrobials and as therapeutic agents against infectious diseases (phage therapy). Phages to be used for t...
Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector [0.03%]
采用自动显微注射仪进行受精卵胞质注射制备CRISPR/Cas9基因修饰小鼠
Brendan Doe,Ellen Brown,Katharina Boroviak
Brendan Doe
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shor...
The Scopoletin-HRP Fluorimetric Determination of H2O2 in Seawaters-A Plea for the Two-Stage Protocol [0.03%]
scopoletin-HRP荧光法测定海水中的H2O2-兼论分步法的重要性
Man Wu,George T F Wong,Yao-Chu Wu
Man Wu
A single solution protocol has been widely used for the fluorimetric determination of H2O2 in natural waters by its bleaching of the fluorescing scopoletin in the presence of the enzyme horseradish peroxidase (HRP). In this protocol, the re...
Predictable Peptide Conjugation Ratios by Activation of Proteins with Succinimidyl Iodoacetate (SIA) [0.03%]
用琥珀酰亚胺基碘乙酸(SIA)活化蛋白质实现可预测的肽连接比例
Ioana M Abbas,Timm Schwaar,Frank Bienwald et al.
Ioana M Abbas et al.
The small heterobifunctional linker succinimidyl iodoacetate (SIA) was examined for the preparation of peptide-protein bioconjugates with predicable conjugation ratios. For many conjugation protocols, the protein is either treated with a re...
Fernando Albericio
Fernando Albericio
I am pleased to introduce Methods and Protocols, a new multidisciplinary, peer-reviewed, open access journal devoted to providing an open forum to report on new procedural approaches and cutting-edge methodological developments.[...].
Fast Proteome Identification and Quantification from Data-Dependent Acquisition-Tandem Mass Spectrometry (DDA MS/MS) Using Free Software Tools [0.03%]
使用免费软件工具从数据依赖性获取-串联质谱（DDA MS/MS）快速鉴定和定量蛋白质组
Jesse G Meyer
Jesse G Meyer
The identification of nearly all proteins in a biological system using data-dependent acquisition (DDA) tandem mass spectrometry has become routine for organisms with relatively small genomes such as bacteria and yeast. Still, the quantific...
Manolo U Rios,Jesse E Bucksot,Kimiya C Rahebi et al.
Manolo U Rios et al.
Peripheral nerve stimulation has emerged as a platform therapy to treat a wide range of disorders. Continued development and translation of these strategies requires that researchers have access to reliable, customizable electrodes for nerv...
A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK) [0.03%]
一种高度敏感的非放射性腺苷酸活化蛋白激酶活性测定方法
Yan Yan,Xin Gu,H Eric Xu et al.
Yan Yan et al.
While many methods exist to quantitatively determine protein kinase activities, 32P-based radioactive assays remain the workhorse of many laboratories due to their high sensitivity, high signal to noise ratio, lack of interference by fluore...
Wild Birds' Genetic Resources Bank: Feather Follicle Cell Culture as a Possible Source of Stem Cells [0.03%]
野生鸟类的遗传资源库：羽毛毛囊细胞培养作为干细胞的一种可能来源
Follicular cells represent a valuable resource for genetic research, biotechnology and cryopreservation in biobanks, particularly for the conservation of endangered species. They offer a more practical alternative to gametes, embryos and fi...
SmilebrightRO-Study Protocol for a Randomized Clinical Trial to Evaluate Oral Health Interventions in Children [0.03%]
儿童口腔保健干预随机临床试验研究方案（SMILEBRIGHT RO）
Background: Oral diseases represent a constant burden for health care and socio-economic systems as they are correlated to other non-communicable diseases. The aim of the proposed intervention is to test the effect of dai...