Easy Employment and Crosstalk-Free Detection of Seven Fluorophores in a Widefield Fluorescence Microscope [0.03%]
一种宽场荧光显微镜中七种荧光染料的易用招聘和无串扰检测方法研究
Sebastian C Bhakdi,Ponpan Thaicharoen
Sebastian C Bhakdi
Immunofluorescence staining has become an essential tool in pathology and biomedical sciences to identify rare cells, cell-cell interactions, and submicroscopic cellular components. Many experimental settings, however, suffer from the fact ...
A Cost-Effective and Efficient Chick Ex-Ovo CAM Assay Protocol to Assess Angiogenesis [0.03%]
一种成本效益高且高效的鸡胚体外培养CAM模型检测血管生成的实验方案
Monali Naik,Pratush Brahma,Manjusha Dixit
Monali Naik
The chick chorioallantoic membrane (CAM) is an extra-embryonic membrane, comprised of a high density of blood and lymphatic vessels. CAM has a dense capillary network and is commonly used to study in vivo angiogenesis and anti-angiogenesis ...
Function of the RNA Coliphage Qβ Proteins in Medical In Vitro Evolution [0.03%]
医学体外进化中大肠杆菌噬菌体Qβ的RNA蛋白质的作用
Rana L Singleton,Carrie A Sanders,Kevin Jones et al.
Rana L Singleton et al.
Qβ is a positive (+) single-stranded RNA bacteriophage covered by a 25 nm icosahedral shell. Qβ belongs to the family of Leviviridae and is found throughout the world (bacterial isolates and sewage). The genome of Qβ is about 4.2 kb, cod...
A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies [0.03%]
一种两步法高效纯化用于基因编辑、生物物理及结构研究的Cas9蛋白
Nandhakishore Rajagopalan,Sateesh Kagale,Pankaj Bhowmik et al.
Nandhakishore Rajagopalan et al.
Cas9 is a site-specific RNA-guided endonuclease (RGEN) that can be used for precise genome editing in various cell types from multiple species. Ribonucleoprotein (RNP) complexes, which contains the Cas9 protein in complex with a guide RNA, ...
CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies [0.03%]
应用于Bicyclus anynana蝴蝶的CRISPR-Cas9基因组编辑技术
Tirtha Das Banerjee,Antónia Monteiro
Tirtha Das Banerjee
CRISPR-Cas9 is revolutionizing the field of genome editing in non-model organisms. The robustness, ease of use, replicability and affordability of the technology has resulted in its widespread adoption among researchers. The African butterf...
Fumihiro Motojima
Fumihiro Motojima
Coomassie brilliant blue (CBB) dyes have been commonly used for the staining of protein bands in polyacrylamide gel electrophoresis (PAGE) gels. However, the staining and destaining of CBB dyes are time-consuming, and the use of methanol is...
Paramyxovirus Infections in Ex Vivo Lung Slice Cultures of Different Host Species [0.03%]
不同宿主物种的体内外肺片培养中的副粘病毒感染
Rory D De Vries,Linda J Rennick,W Paul Duprex et al.
Rory D De Vries et al.
In vivo experiments in animal models of disease are of crucial importance for viral tropism and pathogenesis studies. However, these experiments must be complemented with in vitro and ex vivo experiments. Here, we describe a protocol for th...
Intussusception in Young Children: Protocol for Multisite Hospital Sentinel Surveillance in India [0.03%]
小儿肠套叠哨点监测医院协议——印度多中心试点研究方案
Manoja Kumar Das,Narendra Kumar Arora,Jan Bonhoeffer et al.
Manoja Kumar Das et al.
India has recently introduced a rotavirus vaccine under a universal immunization program. There is limited information on intussusception, an adverse event, following immunization in children from India. We are conducting sentinel surveilla...
High Performance Liquid Chromatography Separation of Epigenetic Cytosine Variants [0.03%]
高效液相色谱分离表观遗传学胞嘧啶变异体
Caroline E Roberts,Gregory M Raner,Gary D Isaacs
Caroline E Roberts
Epigenetic modifications enable cells to genetically respond to chemical inputs from environmental sources. These marks play a pivotal role in normal biological processes (e.g., differentiation, host defense and metabolic programs) but also...
Francesca Ferrara,Nigel Temperton
Francesca Ferrara
Pseudotype neutralization assays are powerful tools to study functional antibody responses against viruses in low biosafety laboratories. However, protocols described in the literature differ widely with respect to material, reagents, and m...