Leonid V Gening,Oleg V Shevchenko,Konstantin Y Kazachenko et al.
Leonid V Gening et al.
We propose an improved method for detecting mutations that arise in DNA due to misincorporations of deoxyadenosine-5'-monophosphate (dAMP) opposite 7,8-dihydro-8-oxoguanine (8-oxoG). The method is based on the synthesis of complementary cha...
Molecular Analysis of PKU-Associated PAH Mutations: A Fast and Simple Genotyping Test [0.03%]
PKU相关PAH突变的分子分析：一种快速简便的基因型检测方法
Manuela Tolve,Cristiana Artiola,Amelia Pasquali et al.
Manuela Tolve et al.
Neonatal screening for phenylketonuria (PKU, OMIM: 261600) was introduced at the end of the 1960s. We developed a rapid and simple molecular test for the most frequent phenylalanine hydroxylase (PAH, Gene ID: 5053) mutations. Using this met...
High-Throughput Genotyping of CRISPR/Cas Edited Cells in 96-Well Plates [0.03%]
基于CRISPR/Cas的高通量基因分型技术及其在96孔板中的应用
Lea Nussbaum,Jelena M Telenius,Stephanie Hill et al.
Lea Nussbaum et al.
The emergence in recent years of DNA editing technologies-Zinc finger nucleases (ZFNs), transcription activator-like effector (TALE) guided nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/Cas family en...
Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors [0.03%]
基于质粒和单链寡核苷酸供体的HUDEP-2红系祖细胞CRISPR/Cas9基因组编辑
Gemma Moir-Meyer,Pak Leng Cheong,Aude-Anais Olijnik et al.
Gemma Moir-Meyer et al.
The study of cellular processes and gene regulation in terminal erythroid development has been greatly facilitated by the generation of an immortalised erythroid cell line derived from Human Umbilical Derived Erythroid Precursors, termed HU...
Džiuginta Jakočiūnė,Arshnee Moodley
Džiuginta Jakočiūnė
Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better ...
Mass Spectrometric Analysis of Bisphenol A Desorption from Titania Nanoparticles: Ammonium Acetate, Fluoride, Formate, and Hydroxide as Chemical Desorption Agents [0.03%]
乙二醇从二氧化钛纳米粒子解吸的质谱分析：氯化铵、氟化物、甲酸盐和氢氧化物作为化学解吸剂
Seyed Mohammad Majedi,Edward P C Lai
Seyed Mohammad Majedi
Bisphenol A (BPA) is a widely used chemical in several consumer products and a well-studied environmental toxicant, and therefore, its accurate measurement is highly demanded. However, the co-presence of nanoparticles as an emerging class o...
An Impact Mapping Method to Generate Robust Qualitative Evaluation of Community-Based Research Programs for Youth and Adults [0.03%]
一种影响映射方法，用于生成针对青年和成年人社区研究项目的稳健定性评价
Melissa D Olfert,Rebecca L Hagedorn,Jade A White et al.
Melissa D Olfert et al.
Ripple Effect Mapping (REM) is an evaluation approach that has traditionally been used in community settings to visually map the impact of programming and community interventions. This manuscript utilizes the Community Capitals Framework (C...
Ionic Liquid-Assisted Laser Desorption/Ionization-Mass Spectrometry: Matrices, Microextraction, and Separation [0.03%]
室温离子液体辅助基质激光解析电离质谱：基质、微萃取及分离
Hani Nasser Abdelhamid
Hani Nasser Abdelhamid
Ionic liquids (ILs) have advanced a variety of applications, including matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). ILs can be used as matrices and solvents for analyte extraction and separation prior to analysi...
A Novel Method for the Combined Photocatalytic Activity Determination and Bandgap Estimation [0.03%]
一种同时评价光催化活性和估计禁带宽度的新方法
Mattia Pierpaoli,Orlando Favoni,Gabriele Fava et al.
Mattia Pierpaoli et al.
The ability of a photocatalyst to degrade a target pollutant is a commonly used method to assess its effectiveness for environmental applications, while ultraviolet-visible (UV-vis) spectroscopy and spectroscopic ellipsometry are convention...
Establishment of Orthotopic Liver Tumors by Surgical Intrahepatic Tumor Injection in Mice with Underlying Non-Alcoholic Fatty Liver Disease [0.03%]
通过手术肝脏内注射在伴有非酒精性脂肪肝病小鼠中建立原位肝肿瘤模型
Zachary J Brown,Bernd Heinrich,Tim F Greten
Zachary J Brown
The prevalence of non-alcoholic fatty liver disease (NAFLD) and its advanced form, nonalcoholic steatohepatitis (NASH), is increasing, and as such its contribution to the development of hepatocellular carcinoma is also rising. NAFLD has bee...