A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor [0.03%]
表皮生长因子受体单颗粒追踪简史
David T Clarke,Marisa L Martin-Fernandez
David T Clarke
Single-particle tracking (SPT) has been used and developed over the last 25 years as a method to investigate molecular dynamics, structure, interactions, and function in the cellular context. SPT is able to show how fast and how far individ...
Large Scale Double-Path Illumination System with Split Field of View for the All-Optical Study of Inter-and Intra-Hemispheric Functional Connectivity on Mice [0.03%]
一种用于小鼠半球间及半球内功能连接全光学研究的大型双通道照明系统（视场可分）
Emilia Conti,Anna Letizia Allegra Mascaro,Francesco Saverio Pavone
Emilia Conti
Recent improvements in optical tools that can perturb brain activity and simultaneously reveal the elicited alterations in the associated regions offer an exceptional means to understand and map the connectivity of the brain. In this work, ...
A Software Architecture to Mimic a Ventricular Tachycardia in Intact Murine Hearts by Means of an All-Optical Platform [0.03%]
一种通过全光学平台模拟小鼠完整心脏室性心动过速的软件架构
Francesco Giardini,Valentina Biasci,Marina Scardigli et al.
Francesco Giardini et al.
Optogenetics is an emerging method that uses light to manipulate electrical activity in excitable cells exploiting the interaction between light and light-sensitive depolarizing ion channels, such as channelrhodopsin-2 (ChR2). Initially use...
AFM-Based Force Spectroscopy Guided by Recognition Imaging: A New Mode for Mapping and Studying Interaction Sites at Low Lateral Density [0.03%]
基于识别成像的原子力显微镜力谱学：一种用于低横向密度相互作用位点映射和研究的新模式
Melanie Koehler,Anny Fis,Hermann J Gruber et al.
Melanie Koehler et al.
Ligand binding to receptors is one of the most important regulatory elements in biology as it is the initiating step in signaling pathways and cascades. Thus, precisely localizing binding sites and measuring interaction forces between cogna...
Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity [0.03%]
一种新型的喷菌法用于抗菌活性的元基因文库功能筛选
Anissa Brahami,Annie Castonguay,Éric Déziel
Anissa Brahami
Metagenomic techniques, notably the cloning of environmental DNA (eDNA) into surrogate hosts, have given access to the genome of uncultured bacteria. However, the determination of gene functions based on DNA sequences alone remains a signif...
Synthesis of Highly Concentrated Suspensions of Silver Nanoparticles by Two Versions of the Chemical Reduction Method [0.03%]
采用化学还原法制备高浓度银纳米粒子悬浮液
Miguel Gakiya-Teruya,Luis Palomino-Marcelo,Juan Carlos F Rodriguez-Reyes
Miguel Gakiya-Teruya
In spite of the widespread use of the chemical reduction method to obtain silver nanoparticles, the nanoparticle yield is often low due to a required addition of small volumes of diluted metal ions to a solution containing a reducer. Higher...
Fast Calculation of Computer Generated Holograms for 3D Photostimulation through Compressive-Sensing Gerchberg-Saxton Algorithm [0.03%]
基于压缩感知Gerchberg-Saxton算法的3D光刺激用计算机生成全息图的快速计算方法
Paolo Pozzi,Laura Maddalena,Nicolò Ceffa et al.
Paolo Pozzi et al.
The use of spatial light modulators to project computer generated holograms is a common strategy for optogenetic stimulation of multiple structures of interest within a three-dimensional volume. A common requirement when addressing multiple...
Quantification of Ethanedinitrile in Air Using a New and Accurate Gas Chromatography Method [0.03%]
一种测定空气中乙二腈的新准确的气相色谱法及定量研究
Sam E Brierley,Anthony R Adlam,Matthew K D Hall
Sam E Brierley
Compared to previously tested fumigants such as methyl bromide, sulfuryl fluoride and phosphine; ethanedinitrile (EDN) is a new fumigant which is being trialled around the world as a pre-plant soil treatment and as a quarantine and pre-ship...
New Protocol for Cell Culture to Obtain Mitotic Chromosomes in Fishes [0.03%]
一种用于鱼类细胞培养以获取有丝分裂染色体的新方法
Fabilene G Paim,Leandro Maia,Fernanda da Cruz Landim-Alvarenga et al.
Fabilene G Paim et al.
Cell culture is an excellent alternative for the maintenance of cell lines and to obtain quality chromosome preparations of fishes. However, this methodology is still little employed, mainly because of the difficulty of standardization of c...
A Method for the Isolation and Characterization of Mycosporine-Like Amino Acids from Cyanobacteria [0.03%]
从蓝藻中分离和鉴定类麦孢素类似氨基酸的方法
Siripat Ngoennet,Yasuhiro Nishikawa,Takashi Hibino et al.
Siripat Ngoennet et al.
This report provides a broadly applicable and cost-effective method for the purification of mycosporine-like amino acids (MAAs) from cyanobacteria. As MAAs are known to have multiple bioactivities for health and beauty, a universal isolatio...