Tania Zaglia,Anna Di Bona,Marco Mongillo
Tania Zaglia
The discovery of optogenetics has revolutionized research in neuroscience by providing the tools for noninvasive, cell-type selective modulation of membrane potential and cellular function in vitro and in vivo. Rhodopsin-based optogenetics ...
Hye Jin Lim,Dong-Myung Kim
Hye Jin Lim
Due to the ongoing crises of fossil fuel depletion, climate change, and environmental pollution, microbial processes are increasingly considered as a potential alternative for cleaner and more efficient production of the diverse chemicals r...
Using Single-Molecule Chemo-Mechanical Unfolding to Simultaneously Probe Multiple Structural Parameters in Protein Folding [0.03%]
利用单分子化学机械变性同时探测蛋白质折叠中的多个结构参数
Emily J Guinn,Susan Marqusee
Emily J Guinn
While single-molecule force spectroscopy has greatly advanced the study of protein folding, there are limitations to what can be learned from studying the effect of force alone. We developed a novel technique, chemo-mechanical unfolding, th...
Jong H Kim,Kathleen L Chan,Luisa W Cheng et al.
Jong H Kim et al.
Current antifungal interventions have often limited efficiency in treating fungal pathogens, particularly those resistant to commercial drugs or fungicides. Antifungal drug repurposing is an alternative intervention strategy, whereby new ut...
Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus [0.03%]
药物作用靶点的加速生成：真核无细胞系统成为焦点
Lena Thoring,Anne Zemella,Doreen Wüstenhagen et al.
Lena Thoring et al.
In the biopharmaceutical pipeline, protein expression systems are of high importance not only for the production of biotherapeutics but also for the discovery of novel drugs. The vast majority of drug targets are proteins, which need to be ...
Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line [0.03%]
多柔比星干扰MCF-7乳腺癌细胞系MTT测定的避免方法研究
Carla Luis,Yuselis Castaño-Guerrero,Raquel Soares et al.
Carla Luis et al.
Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell prol...
A Loose Parts Randomized Controlled Trial to Promote Active Outdoor Play in Preschool-aged Children: Physical Literacy in the Early Years (PLEY) Project [0.03%]
学龄前儿童户外活动促进——玩耍物理素养项目(PLEY)
Natalie E Houser,Jane Cawley,Angela M Kolen et al.
Natalie E Houser et al.
Background: The Physical Literacy in the Early Years (PLEY) intervention is a randomized mixed-methods controlled trial focused on embedding loose parts materials into the outdoor play spaces of regulated child care centr...
Characterizing the Anoxic Phenotype of Pseudomonas putida Using a Bioelectrochemical System [0.03%]
采用一种生物电化学系统表征Pseudomonas putida的缺氧表型
Bin Lai,Anh Vu Nguyen,Jens O Krömer
Bin Lai
Industrial fermentation in aerobic processes is plagued by high costs due to gas transfer limitations and substrate oxidation to CO2. It has been a longstanding challenge to engineer an obligate aerobe organism, such as Pseudomonas putida, ...
Why RGB Imaging Should be Used to Analyze Fusarium Graminearum Growth and Estimate Deoxynivalenol Contamination [0.03%]
为什么应该使用RGB成像来分析致病菌生长和估算脱氧雪腐镰刀菌烯醇污染程度
Edgar Cambaza,Shigenobu Koseki,Shuso Kawamura
Edgar Cambaza
Size-based fungal growth studies are limited because they do not provide information about the mold's state of maturity, and measurements such as radius and diameter are not practical if the fungus grows irregularly. Furthermore, the curren...
Nicole E Gregorio,Max Z Levine,Javin P Oza
Nicole E Gregorio
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression ...