Induction of a Th17 Phenotype in Human Skin-A Mimic of Dermal Inflammatory Diseases [0.03%]
诱导人类皮肤的Th17表型-一种模拟真皮炎性疾病的方法
Sara M Garrett,Qihong Zhao,Carol Feghali-Bostwick
Sara M Garrett
Th17 cells are a subset of effector T helper cells that produce interleukin (IL)-17A, IL-17F, IL-22, and IL-26, which can promote tissue inflammation and contribute to the pathogenesis of rheumatic, fibrosing, and other diseases. Research i...
MALDI Mass Spectrometry Imaging Linked with Top-Down Proteomics as a Tool to Study the Non-Small-Cell Lung Cancer Tumor Microenvironment [0.03%]
用于研究非小细胞肺癌肿瘤微环境的基质辅助激光解析电离质量成像与顶视蛋白质组学相结合的方法
Eline Berghmans,Geert Van Raemdonck,Karin Schildermans et al.
Eline Berghmans et al.
Advanced non-small-cell lung cancer (NSCLC) is generally linked with a poor prognosis and is one of the leading causes of cancer-related deaths worldwide. Since only a minority of the patients respond well to chemotherapy and/or targeted th...
A Combined AFM and Lateral Stretch Device Enables Microindentation Analyses of Living Cells at High Strains [0.03%]
结合AFM和拉伸装置可实现活细胞高应变微压痕分析
Dave Ahrens,Wolfgang Rubner,Ronald Springer et al.
Dave Ahrens et al.
Mechanical characterization of living cells undergoing substantial external strain promises insights into material properties and functional principles of mechanically active tissues. However, due to the high strains that occur in physiolog...
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock [0.03%]
原生质体中蓝细菌昼夜钟的实时荧光各向异性检测
Joel Heisler,Archana Chavan,Yong-Gang Chang et al.
Joel Heisler et al.
Uniquely, the circadian clock of cyanobacteria can be reconstructed outside the complex milieu of live cells, greatly simplifying the investigation of a functioning biological chronometer. The core oscillator component is composed of only t...
Co-Designing an Intervention to Increase HIV Testing Uptake with Women from Indonesia At-Risk of HIV: Protocol for a Participatory Action Research Study [0.03%]
与印度尼西亚易感染艾滋病毒的妇女共同设计一种增加艾滋病检测接受率的干预措施：参与性行动研究方案.protocol for a participatory action research study)
Corie Gray,Gemma Crawford,Roanna Lobo et al.
Corie Gray et al.
Early diagnosis is a critical component of the global response to the human immunodeficiency virus (HIV). In Australia, more than two-thirds of women from Southeast Asia are diagnosed late with HIV. There is limited evidence regarding the b...
Improved Method for DNA Extraction and Purification from Tetrahymena pyriformis [0.03%]
改进的四膜虫DNA提取和纯化方法
Ezzouhra El Maaiden,Youssef El Kharrassi,Abdel Khalid Essamadi et al.
Ezzouhra El Maaiden et al.
Tetrahymena pyriformis (protozoa) is intensely investigated as a model organism, offering numerous advantages in comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is a vital step of any...
Cell-Free Synthetic Biology Platform for Engineering Synthetic Biological Circuits and Systems [0.03%]
无细胞合成生物学平台用于工程化合成生物线路和系统
Dohyun Jeong,Melissa Klocke,Siddharth Agarwal et al.
Dohyun Jeong et al.
Synthetic biology brings engineering disciplines to create novel biological systems for biomedical and technological applications. The substantial growth of the synthetic biology field in the past decade is poised to transform biotechnology...
Study Protocol for a Pilot, Open-Label, Prospective, and Observational Study to Evaluate the Pharmacokinetics of Drugs Administered to Patients during Extracorporeal Circulation; Potential of In Vivo Cytochrome P450 Phenotyping to Optimise Pharmacotherapy [0.03%]
体外循环过程中给药患者的药物代谢动力学研究方案——潜在的体内细胞色素P450表型测定以优化药物治疗
Santosh Kumar Sreevatsav Adiraju,Kiran Shekar,Peter Tesar et al.
Santosh Kumar Sreevatsav Adiraju et al.
Pharmacokinetic alterations of medications administered during surgeries involving cardiopulmonary bypass (CPB) and extracorporeal membrane oxygenation (ECMO) have been reported. The impact of CPB on the cytochrome P450 (CYP) enzymes' activ...
Electroextraction of Insoluble Proteins from the Organic Matrix of the Nacreous Layer of the Japanese Pearl Oyster, Pinctada fucata [0.03%]
日本真珠贝棱柱层有机基质中难溶蛋白的电提取
Daisuke Funabara,Natsumi Miyashita,Kiyohito Nagai et al.
Daisuke Funabara et al.
The nacreous layer of shells and pearls is composed of aragonite crystals arranged in an organic matrix. The organic matrix contains chitin and several proteins that regulate the formation of the nacreous layer. Owing to their strong intera...
Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification [0.03%]
优化从单个白蛉中提取DNA以用于PCR扩增的方法
Lorena G Caligiuri,Adolfo E Sandoval,Jose C Miranda et al.
Lorena G Caligiuri et al.
Numerous protocols have been published for extracting DNA from phlebotomines. Nevertheless, their small size is generally an issue in terms of yield, efficiency, and purity, for large-scale individual sand fly DNA extractions when using tra...