Sampling Methods for Luminescence Dating of Subsurface Deposits from Cores [0.03%]
取芯沉积物发光测年的采样方法研究进展
Michelle Nelson,Tammy Rittenour,And Harriet Cornachione
Michelle Nelson
Study of subsurface deposits often requires coring or drilling to obtain samples for sedimentologic and geochemical analysis. Geochronology is a critical piece of information for stratigraphic correlation and rate calculations. Increasingly...
Transferring Grains from Single-Grain Luminescence Discs to SEM Specimen Stubs [0.03%]
从单粒光释光盘向扫描电镜样品杆转移样品的方法
Isa Doverbratt,Helena Alexanderson
Isa Doverbratt
The grain transfer protocol presents a step-by-step guide on how to successfully transfer positioned grains from a single-grain luminescence disc to a scanning electron microscope (SEM) specimen stub and how to transport them between labora...
Characterising Mutational Spectra of Carcinogens in the Tumour Suppressor Gene TP53 Using Human TP53 Knock-in (Hupki) Mouse Embryo Fibroblasts [0.03%]
用人类TP53基因敲入小鼠胚胎成纤维细胞表征致癌剂在肿瘤抑制基因TP53中的突变谱型
Lisa Hölzl-Armstrong,Jill E Kucab,Michael Korenjak et al.
Lisa Hölzl-Armstrong et al.
DNA in dividing cells is prone to mutagenesis, with mutations making key contributions to human disease including cancer. The tumour suppressor gene TP53 is the most frequently mutated gene in human tumours. Here, we present a robust protoc...
A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis [0.03%]
一种重复利用归档的H&E染色组织切片进行多重蛋白生物标志物分析的方法
James P Hinton,Katerina Dvorak,Esteban Roberts et al.
James P Hinton et al.
Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in stu...
High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro [0.03%]
hypertrophic心肌病细胞模型的高通量表型工具包用于体外特征分析
Diogo Mosqueira,Katarzyna Lis-Slimak,Chris Denning
Diogo Mosqueira
Hypertrophic cardiomyopathy (HCM) is a prevalent and complex cardiovascular disease characterised by multifarious hallmarks, a heterogeneous set of clinical manifestations, and several molecular mechanisms. Various disease models have been ...
Alkaline Extraction for Lead Determination in Different Types of Commercial Paints [0.03%]
不同类型油画的碱性提取铅测定法
David Romero-Estévez,Gabriela S Yánez-Jácome,Karina Simbaña-Farinango et al.
David Romero-Estévez et al.
In 2017, the World Health Organization and the United Nations Environment Programme formed the Global Alliance to Eliminate Lead Paint. All alliance member countries have pledged to develop control regulations that include lead threshold li...
Assessing Pancreas Transplant Candidate Cardiac Disease: Preoperative Protocol Development at a Rapidly Growing Transplant Program [0.03%]
胰腺移植供体心脏病评估：一个快速增长的移植中心术前方案制定
David St Michel,Tracy Donnelly,Towanda Jackson et al.
David St Michel et al.
Pancreas transplant rates, despite improving outcomes, have decreased over the past two decades. This is due, in part, to ageing, increasingly co-morbid pancreas transplant candidates. There is a paucity of published data regarding coronary...
Simple and Quick Visualization of Periodical Data Using Microsoft Excel [0.03%]
使用Microsoft Excel简便快捷地可视化周期性数据
Hideaki Oike,Yukino Ogawa,Katsutaka Oishi
Hideaki Oike
Actograms are well-established methods used for visualizing periodic activity of animals in chronobiological research. They help in the understanding of the overall characteristics of rhythms and are instrumental in defining the direction o...
"Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis [0.03%]
“无细胞合成生物学”：合成生物学遇见无细胞蛋白合成
Seok Hoon Hong
Seok Hoon Hong
Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [1], the technology has been developed over the course of decades and applied to studying both fundamental and applied bio...
Isolation, Maintenance and Differentiation of Primary Tracheal Basal Cells from Adult Rhesus Macaque [0.03%]
成人恒河猴主气管基底细胞的分离、纯化和分化诱导
Anna E Engler,Gustavo Mostoslavsky,Lisa Miller et al.
Anna E Engler et al.
In this report, we describe methodologies for the isolation and culture of primary rhesus macaque tracheal basal cells, their cryopreservation, long term storage and differentiation. These are comparable to state-of-the-art protocols that h...