Positive Selection of Specific Antibodies Produced against Fusion Proteins [0.03%]
针对融合蛋白产生特异抗体的正向选择
Lukas Kramberger-Kaplan,Tina Austerlitz,Holger Bohlmann
Lukas Kramberger-Kaplan
A method for the positive selection of specific antibodies for target proteins expressed as fusion proteins for the production of antiserum is presented. As proof of concept, the fusion protein FLAG::His::GFP::His::FLAG was expressed in Esc...
High-Throughput Fingerprinting of Rhizobial Free Fatty Acids by Chemical Thin-Film Deposition and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry [0.03%]
基于化学薄膜沉积和基质辅助激光解析电离傅里叶变换离子回旋共振质量测定的根瘤菌游离脂肪酸高通量指纹分析法
Aleksey Gladchuk,Julia Shumilina,Alena Kusnetsova et al.
Aleksey Gladchuk et al.
Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural di...
Validation of a Simple HPLC-UV Method for the Determination of Monomers Released from Dental Resin Composites in Artificial Saliva [0.03%]
一种测定模拟唾液中牙树脂复合材料释放单体的HPLC-UVDetermination方法的验证
Elisavet-Ioanna Diamantopoulou,Orfeas-Evanggelos Plastiras,Petros Mourouzis et al.
Elisavet-Ioanna Diamantopoulou et al.
Bisphenol-A (BPA), bisphenol A glycerolate dimethacrylate (Bis-GMA), triethylene glycol dimethacrylate (TEGDMA), and urethane dimethacrylate (UDMA) are organic monomers that can be released from dental composites into the oral cavity. Over ...
OCTAVVS: A Graphical Toolbox for High-Throughput Preprocessing and Analysis of Vibrational Spectroscopy Imaging Data [0.03%]
OCTAVVS：一个用于高通量处理和分析振动光谱成像数据的图形工具箱
Carl Troein,Syahril Siregar,Michiel Op De Beeck et al.
Carl Troein et al.
Modern vibrational spectroscopy techniques enable the rapid collection of thousands of spectra in a single hyperspectral image, allowing researchers to study spatially heterogeneous samples at micrometer resolution. A number of algorithms h...
Combined Fluorescence-Based in Vitro Assay for the Simultaneous Detection of Cell Viability and Alkaline Phosphatase Activity during Osteogenic Differentiation of Osteoblast Precursor Cells [0.03%]
一种联合的荧光体外检测法：在成骨祖细胞分化过程中同时检测细胞活力和碱性磷酸酶活性
Sebastian Wilkesmann,Fabian Westhauser,Joerg Fellenberg
Sebastian Wilkesmann
Novel bone substitute materials need to be evaluated in terms of their osteogenic differentiation capacity and possible unwanted cytotoxic effects in order to identify promising candidates for the therapy of bone defects. The activity of al...
Analysis of GPI-Anchored Receptor Distribution and Dynamics in Live Cells by Tag-mediated Enzymatic Labeling and FRET [0.03%]
基于标签的酶原位标记和FRET分析活细胞中GPI锚定受体分布与动态变化
Maria N Balatskaya,Alexandra I Baglay,Yury P Rubtsov et al.
Maria N Balatskaya et al.
The analysis of glycosylphosphatidylinositol (GPI)-anchored receptor distribution and dynamics in live cells is challenging, because their clusters exhibit subdiffraction-limited sizes and are highly dynamic. However, the cellular response ...
Monitoring Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction [0.03%]
通过探究线粒体功能障碍监测胰腺癌细胞的上皮间质转化
Jae Jun Sim,Keun-Yeong Jeong
Jae Jun Sim
In this protocol, we introduced a method of measuring mitochondrial dysfunction to confirm the epithelial-mesenchymal transition (EMT) in pancreatic cancer cells under a hypoxic environment. There are many expertized and complicated methods...
Mujeeb Shittu,Tessa Steenwinkel,Shigeyuki Koshikawa et al.
Mujeeb Shittu et al.
The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes. Studies regarding these processes depend on overexpression and down...
Quantifying Physical Thrombus Characteristics on Cardiovascular Biomaterials Using MicroCT [0.03%]
基于微焦斑CT的血管材料表面血栓物理特性的表征量化研究
Avi Gupta,Claire M Johnston,Monica T Hinds et al.
Avi Gupta et al.
Hemocompatibility is a critical consideration when designing cardiovascular devices. Methods of assessing hemocompatibility range from in vitro protein adsorption and static platelet attachment to in vivo implantation. A standard preclinica...
Mapping Quantitative Trait Loci onto Chromosome-Scale Pseudomolecules in Flax [0.03%]
基于纤维植物亚麻基因组精细图的QTL作图研究
Frank M You,Sylvie Cloutier
Frank M You
Quantitative trait loci (QTL) are genomic regions associated with phenotype variation of quantitative traits. To date, a total of 313 QTL for 31 quantitative traits have been reported in 14 studies on flax. Of these, 200 QTL from 12 studies...