Comparison of DNA Extracted from Pediatric Saliva, Gingival Crevicular Fluid and Site-Specific Biofilm Samples [0.03%]
儿童唾液、牙龈 crevicular 液和位点特异性菌斑样本中提取的 DNA 的比较
Jason Emett,Roxanne David,Jaydene McDaniel et al.
Jason Emett et al.
(1) Introduction: Due to the non-invasive nature of saliva, many methods have been used to isolate and collect DNA from saliva samples for microbial screening. Many oral microbes also inhabit the oral biofilm, which may represent significan...
Michael Koonce,Irina Tikhonenko,Ralph Gräf
Michael Koonce
We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. ...
Stage Specific Expression Pattern of Alpha-Hemoglobin-Stabilizing-Protein (AHSP) Portrayed in Erythroblast Chronology [0.03%]
红系细胞发育过程中α-珠蛋白稳定蛋白(AHSP)的表达模式研究
Julia Walczak,Maria D Camargo Johnson,Kuzhali Muthumalaiappan
Julia Walczak
During erythropoiesis, the molecular chaperone alpha-hemoglobin-stabilizing protein (AHSP) sequesters free alpha-hemoglobin (αHb) and prevents precipitation of excess αHb. While AHSP is linked to hereditary anemia, the pattern of expressi...
Optimization of a Digestion Method to Determine Total Mercury in Fish Tissue by Cold Vapor Atomic Fluorescence Spectrophotometry [0.03%]
冷原子荧光法测定鱼类样品中总汞消化方法的优化
Gabriela S Yánez-Jácome,David Romero-Estévez,Hugo Navarrete et al.
Gabriela S Yánez-Jácome et al.
Several microwave-assisted digestion methods were tested at the Centro de Estudios Aplicados en Química laboratory in Quito, Ecuador, to determine the accuracy and performance efficiency of the mineralization process for the determination ...
Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine [0.03%]
一锅法制备戒毒药甲基苯丙胺的合成淬灭体传感器
Hee-Jin Jeong,Jinhua Dong,Chang-Hun Yeom et al.
Hee-Jin Jeong et al.
The problem of illicit drug use and addiction is an escalating issue worldwide. As such, fast and precise detection methods are needed to help combat the problem. Herein, the synthesis method for an anti-methamphetamine Quenchbody (Q-body),...
Production and Purification of Artificial Circular RNA Sponges for Application in Molecular Biology and Medicine [0.03%]
人工环形RNA海绵的制备与纯化及其在分子生物学和医学中的应用
Janina Breuer,Oliver Rossbach
Janina Breuer
Characterized by their covalently closed structure and thus an elevated stability compared to linear RNA molecules, circular RNAs (circRNAs) form a novel class of mainly non-coding RNAs. Although the biological functions of naturally occurr...
A Simple and Fast Manual Centrifuge to Spin Solutions in 96-Well PCR Plates [0.03%]
一种简单快速的手动离心机用于96孔PCR板溶液的离心
Ken Motohashi
Ken Motohashi
A simple and fast manual centrifuge was developed to spin down solutions in 96-well polymerase chain reaction (PCR) plates. A commercially available salad spinner was utilized for this purpose. Acceleration and deceleration of the centrifug...
Analysis of the Level of Plasmid-Derived mRNA in the Presence of Residual Plasmid DNA by Two-Step Quantitative RT-PCR [0.03%]
采用两步定量RT-PCR分析残留质粒DNA存在时质粒表达的mRNA水平
Barbara Ahlemeyer,Claudia Colasante,Eveline Baumgart-Vogt
Barbara Ahlemeyer
In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA. In our case, mouse calvarial osteobla...
The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles [0.03%]
DNA提取和脂质去除对母乳细菌谱系的影响
Anna Ojo-Okunola,Shantelle Claassen-Weitz,Kilaza S Mwaikono et al.
Anna Ojo-Okunola et al.
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial...
Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary? [0.03%]
生物活性玻璃在静态细胞培养前的预处理：什么才是必要的？
Frederike Hohenbild,Marcela Arango-Ospina,Arash Moghaddam et al.
Frederike Hohenbild et al.
Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches, such...