Comparison of Opioid-Free Anesthesia Versus Opioid-Containing Anesthesia for Elective Laparoscopic Surgery (COFA: LAP): A Protocol Measuring Recovery Outcomes [0.03%]
无阿片麻醉与含阿片麻醉在择期腹腔镜手术中效果的对比（ cofa：lap）：测量恢复结果的一种方案
Anthony Eidan,Angela Ratsch,Elizabeth A Burmeister et al.
Anthony Eidan et al.
The administration of opioids is a central element in contemporary anesthetic techniques in Australia; however, opioids have a range of side effects. As an alternative, opioid-free anesthesia (OFA) is an emerging mode of anesthesia intended...
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent [0.03%]
使用 Viafect™ 转染试剂转染 hPSC-心肌细胞
Sara E Bodbin,Chris Denning,Diogo Mosqueira
Sara E Bodbin
Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfec...
Pooja Sharma,Van K Lam,Christopher B Raub et al.
Pooja Sharma et al.
Motility is a key property of a cell, required for several physiological processes, including embryonic development, axon guidance, tissue regeneration, gastrulation, immune response, and cancer metastasis. Therefore, the ability to examine...
Quantitative Microdialysis: Experimental Protocol and Software for Small Molecule Protein Affinity Determination and for Exclusion of Compounds with Poor Physicochemical Properties [0.03%]
定量微透析：用于小分子蛋白亲和力测定及排除物理化学性质较差化合物的实验方案和软件
Steven Shave,Nhan T Pham,Connor B Śmieja et al.
Steven Shave et al.
Quantitative microdialysis is a traditional biophysical affinity determination technique. In the development of the detailed experimental protocol presented, we used commercially available equipment, rapid equilibrium dialysis (RED) devices...
IsoSearch: An Untargeted and Unbiased Metabolite and Lipid Isotopomer Tracing Strategy from HR-LC-MS/MS Datasets [0.03%]
IsoSearch：来自HR-LC-MS/MS数据集的非靶向和无偏代谢物和脂质同位素追踪策略
He Huang,Min Yuan,Phillip Seitzer et al.
He Huang et al.
Stable isotopic tracer analysis is a technique used to determine carbon or nitrogen atom incorporation into biological systems. A number of mass spectrometry based approaches have been developed for this purpose, including high-resolution t...
Unexpectedly High Levels of Inverted Re-Insertions Using Paired sgRNAs for Genomic Deletions [0.03%]
使用配对sgRNA进行基因组删除时意外高水平的反向重新连接
Joseph Blayney,Evangeline M Foster,Marta Jagielowicz et al.
Joseph Blayney et al.
Use of dual sgRNAs is a common CRISPR/Cas9-based strategy for the creation of genetic deletions. The ease of screening combined with a rather high rate of success makes this approach a reliable genome engineering procedure. Recently, a numb...
Marta Rodríguez-Martínez,Stephanie A Hills,John F X Diffley et al.
Marta Rodríguez-Martínez et al.
Measuring differences in cell cycle progression is often essential to understand cell behavior under different conditions, treatments and environmental changes. Cell synchronization is widely used for this purpose, but unfortunately, there ...
Three-Dimensional Radiographic Evaluation of the Malar Bone Engagement Available for Ideal Zygomatic Implant Placement [0.03%]
理想的颧牙种植体植入的面骨参与的三维放射图评价
Gerardo Pellegrino,Francesco Grande,Agnese Ferri et al.
Gerardo Pellegrino et al.
Zygomatic implant rehabilitation is a challenging procedure that requires an accurate prosthetic and implant plan. The aim of this study was to evaluate the malar bone available for three-dimensional zygomatic implant placement on the possi...
Engineering and Production of the Light-Driven Proton Pump Bacteriorhodopsin in 2D Crystals for Basic Research and Applied Technologies [0.03%]
光驱动质子泵视紫红质在二维晶体中的工程设计与生产（用于基础研究及应用技术）
Mirko Stauffer,Stephan Hirschi,Zöhre Ucurum et al.
Mirko Stauffer et al.
The light-driven proton pump bacteriorhodopsin (BR) from the extreme halophilic archaeon Halobacterium salinarum is a retinal-binding protein, which forms highly ordered and thermally stable 2D crystals in native membranes (termed purple me...
Comparing Four Question Formats in Five Languages for On-Line Consumer Surveys [0.03%]
五种语言的网上消费者调查四种提问方式之比较
Denis Richard Seninde,Edgar Chambers Iv
Denis Richard Seninde
Question formats are critical to the collection of consumer health attitudes, food product characterizations, and perceptions. The information from those surveys provides important insights in the product development process. Four formats b...