CRISPR-Based Editing Techniques for Genetic Manipulation of Primary T Cells [0.03%]
基于CRISPR的初级T细胞基因操作编辑技术
Mateusz Kotowski,Sumana Sharma
Mateusz Kotowski
While clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing techniques have been widely adapted for use in immortalised immune cells, efficient manipulation of primary T cells has proved to be more challeng...
Building Accurate Intracellular Polarity Maps through Multiparametric Microscopy [0.03%]
基于多参数显微镜技术构建精确的细胞内极性图谱
M Carmen Gonzalez-Garcia,Pilar Herrero-Foncubierta,Emilio Garcia-Fernandez et al.
M Carmen Gonzalez-Garcia et al.
The precise knowledge of intracellular polarity, a physiological parameter that involves complex and intertwined intracellular mechanisms, may be relevant in the study of important diseases like cancer or Alzheimer's. In this technical note...
Prediction of Lung Function in Adolescence Using Epigenetic Aging: A Machine Learning Approach [0.03%]
基于表观遗传衰老的青少年肺功能预测：一种机器学习方法
Md Adnan Arefeen,Sumaiya Tabassum Nimi,M Sohel Rahman et al.
Md Adnan Arefeen et al.
Epigenetic aging has been found to be associated with a number of phenotypes and diseases. A few studies have investigated its effect on lung function in relatively older people. However, this effect has not been explored in the younger pop...
Sustainable Management of Organic Wastes in Sharjah, UAE through Co-Composting [0.03%]
通过协同堆肥化可持续管理阿联酋沙迦的有机废弃物
Md Maruf Mortula,Aqeel Ahmed,Kazi Parvez Fattah et al.
Md Maruf Mortula et al.
Daily human activities and vast green areas produce substantial amounts of organic wastes that end up in landfills with minimal treatment. In addition to the problems associated with landfills, disposal through this method is unsustainable ...
Dynamic Navigation for Zygomatic Implants: A Case Report about a Protocol with Intraoral Anchored Reference Tool and an Up-To-Date Review of the Available Protocols [0.03%]
动态导航下种植位点制备的颧牙槽突导板的设计与应用——1例临床病例及最新文献综述
Gerardo Pellegrino,Giuseppe Lizio,Francesco Basile et al.
Gerardo Pellegrino et al.
Dynamic Navigation is a computer-aided technology that allows the surgeon to track the grip instruments while preparing the implant site in real time based on radiological anatomy and accurate pre-operative planning. The support of this tec...
Development of In Vitro Corneal Models: Opportunity for Pharmacological Testing [0.03%]
体外角膜模型的开发：药物测试的新机遇
Valentina Citi,Eugenia Piragine,Simone Brogi et al.
Valentina Citi et al.
The human eye is a specialized organ with a complex anatomy and physiology, because it is characterized by different cell types with specific physiological functions. Given the complexity of the eye, ocular tissues are finely organized and ...
Effects of Implementing the Timed and Targeted Counselling Model on Pregnancy Outcomes and Newborn Survival in Rural Uganda: Protocol for a Quasi-Experimental Study [0.03%]
关于在乌干达农村实施限时且目标明确的咨询模式对妊娠结局和新生儿生存的影响：准实验性研究方案
Geoffrey Babughirana,Sanne Gerards,Alex Mokori et al.
Geoffrey Babughirana et al.
Background: Although mortality rates have declined in Uganda over the last decade, maternal mortality is still high at 336 deaths per 100,000 live births, as is infant mortality at 43 deaths per 1000 live births. One in e...
Cell Dissociation from Butterfly Pupal Wing Tissues for Single-Cell RNA Sequencing [0.03%]
蝴蝶蛹期翅组织的细胞解离以进行单细胞RNA测序
Anupama Prakash,Antónia Monteiro
Anupama Prakash
Butterflies are well known for their beautiful wings and have been great systems to understand the ecology, evolution, genetics, and development of patterning and coloration. These color patterns are mosaics on the wing created by the tilin...
Ultrasonic Navigation for the Treatment of Medication-Related Jaw Osteonecrosis Involving the Inferior Alveolar Nerve: A Case Report and Protocol Review [0.03%]
药物相关下颌骨坏死累及下牙槽神经的超声导航治疗：病例报告和方案综述
Gerardo Pellegrino,Francesca Pavanelli,Agnese Ferri et al.
Gerardo Pellegrino et al.
Dynamic navigation (DN) is a computer-guided technique employed in different surgical fields and recently adopted in dental implantology to improve the accuracy of dental implant insertion. Medication-related osteonecrosis of the jaws (MRON...
An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures [0.03%]
用于哺乳动物细胞培养转染的质粒DNA的有效分离方法
Daniel V Kachkin,Julia I Khorolskaya,Julia S Ivanova et al.
Daniel V Kachkin et al.
In this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell cu...