Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell Cultures [0.03%]
通过SARS-CoV-2感染原发性呼吸道上皮细胞系培养失活材料
Kaitlyn A Barrow,Lucille M Rich,Elizabeth R Vanderwall et al.
Kaitlyn A Barrow et al.
Given that the airway epithelium is the initial site of infection, study of primary human airway epithelial cells (AEC) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) will be crucial to improved understanding of ...
Application of microRNA Database Mining in Biomarker Discovery and Identification of Therapeutic Targets for Complex Disease [0.03%]
基于microRNA数据库挖掘的复杂疾病生物标志物发现及治疗靶点识别的研究应用
Jennifer L Major,Rushita A Bagchi,Julie Pires da Silva
Jennifer L Major
Over the past two decades, it has become increasingly evident that microRNAs (miRNA) play a major role in human diseases such as cancer and cardiovascular diseases. Moreover, their easy detection in circulation has made them a tantalizing t...
An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines in Bombyx mori [0.03%]
一种高效筛选稳定家蚕CRISPR/Cas9基因编辑突变体的方法
Daniel Brady,Alessio Saviane,Silvia Cappellozza et al.
Daniel Brady et al.
The domestic silkworm Bombyx mori is extensively studied as a model organism for lepidopteran genetics and has an economic value in silk production. Silkworms also have applications in biomedical and cosmetic industries, and the production ...
Purification and Characterization of Recombinant Expressed Apple Allergen Mal d 1 [0.03%]
苹果过敏原Mal d 1的纯化及鉴定
Julia A H Kaeswurm,Bettina Nestl,Sven M Richter et al.
Julia A H Kaeswurm et al.
Mal d 1 is the primary apple allergen in northern Europe. To explain the differences in the allergenicity of apple varieties, it is essential to study its properties and interaction with other phytochemicals, which might modulate the allerg...
Predictive Quantitative Structure-Activity Relationship Modeling of the Antifungal and Antibiotic Properties of Triazolothiadiazine Compounds [0.03%]
三唑噻二嗪化合物抗真菌和抗生素属性的预测及定量结构活性关系模型研究
Michael Appell,David L Compton,Kervin O Evans
Michael Appell
Predictive models were developed using two-dimensional quantitative structure activity relationship (QSAR) methods coupled with B3LYP/6-311+G** density functional theory modeling that describe the antimicrobial properties of twenty-four tri...
Giulia Riolo,Silvia Cantara,Carlotta Marzocchi et al.
Giulia Riolo et al.
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression in both animals and plants. By pairing to microRNA responsive elements (mREs) on target mRNAs, miRNAs play gene-regulatory roles, producing remarkable changes in seve...
Digital Approach for the Rehabilitation of the Edentulous Maxilla with Pterygoid and Standard Implants: The Static and Dynamic Computer-Aided Protocols [0.03%]
数字化导板技术在上颌无牙种植修复中的应用（静态和动态计算机辅助导航设计）
Alessio Franchina,Luigi Vito Stefanelli,Simone Gorini et al.
Alessio Franchina et al.
A full-arch rehabilitation of the edentulous upper jaw without grafting procedures exploits the residual alveolar or the basal bone, with the necessity of long implants placed with a particular orientation. The precision in planning and pla...
Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression System [0.03%]
利用T7表达系统高效表达融合核心亲和素的重组可溶蛋白
Ammar Tarar,Esmael M Alyami,Ching-An Peng
Ammar Tarar
The limited amount of fusion protein transported into cytosol milieu has made it challenging to obtain a sufficient amount for further applications. To avoid the laborious and expensive task, T7 promoter-driving pET-30a(+) coding for chimer...
Quantitative, High-Throughput Assays for Proteolytic Degradation of Amylin [0.03%]
用于量化淀粉样蛋白酶解降解的高通量测定法
Caitlin N Suire,Monica K Brizuela,Malcolm A Leissring
Caitlin N Suire
Amylin is a pancreatic peptide hormone that regulates glucose homeostasis but also aggregates to form islet amyloid in type-2 diabetes. Given its role in both health and disease, there is renewed interest in proteolytic degradation of amyli...
In Situ DNA/Protein Interaction Assay to Visualize Transcriptional Factor Activation [0.03%]
用于可视化转录因子活化的原位DNA/蛋白质相互作用测定法
Michela Corsini,Emanuela Moroni,Cosetta Ravelli et al.
Michela Corsini et al.
The chick embryo chorioallantoic membrane (CAM) represents a powerful in vivo model to study several physiological and pathological processes including inflammation and tumor progression. Nevertheless, the possibility of deepening the molec...