Specific Assay of Negative Strand Template to Quantify Intracellular Levels of Rhinovirus Double-Stranded RNA [0.03%]
特异性测定负链模板以量化鼻病毒双链RNA的细胞内水平
Shahina Wiehler,David Proud
Shahina Wiehler
Human rhinovirus infections are a major trigger for acute exacerbations of lower airway diseases, including asthma and chronic obstructive pulmonary disease. Disease exacerbation is thought to be regulated via double-stranded RNA (dsRNA)-me...
The Enzyme-Modified Neutral Comet (EMNC) Assay for Complex DNA Damage Detection [0.03%]
基于酶修饰的中性彗星实验（EM-Nickel COMET）检测复杂DNA损伤
Maria Rita Fabbrizi,Jonathan R Hughes,Jason L Parsons
Maria Rita Fabbrizi
The comet assay is a versatile, simple, and sensitive gel electrophoresis-based method that can be used to measure and accurately quantify DNA damage, particularly single and double DNA strand breaks, in single cells. While generally this i...
The Protocol Gap [0.03%]
协议缺口
Michael G Weller
Michael G Weller
Although peer review is considered one of the main pillars of modern science, experimental methods and protocols seem to be not a rigorous subject of this process in many papers. Commercial equipment, test kits, labeling kits, previously pu...
The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies [0.03%]
中枢神经系统恶性肿瘤的流式细胞术的过去、现在和未来
Evrysthenis Vartholomatos,George Vartholomatos,George A Alexiou et al.
Evrysthenis Vartholomatos et al.
Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as...
Novel In Vivo Mouse Cryoablation Model to Explore Unique Therapeutic Approaches for Premalignant Columnar Lesions [0.03%]
探索前癌性柱状病变独特治疗方案的新颖体内小鼠冷冻消融模型
Ana C P Correia,Danielle Straub,Silvia Calpe et al.
Ana C P Correia et al.
Patients with epithelial metaplasias have an increased risk of developing malignancies. In Barrett's esophagus, neo-columnar epithelium develops proximal to the squamous-columnar junction (SCJ) in the esophagus as the result of prolonged ex...
Ishani Dasgupta,Anushila Chatterjee
Ishani Dasgupta
MicroRNAs (miRNAs) represent a family of short non-coding regulatory RNA molecules that are produced in a tissue and time-specific manner to orchestrate gene expression post-transcription. MiRNAs hybridize to target mRNA(s) to induce transl...
Validity and Cost-Effectiveness of Pediatric Home Respiratory Polygraphy for the Diagnosis of Obstructive Sleep Apnea in Children: Rationale, Study Design, and Methodology [0.03%]
儿童阻塞性睡眠呼吸暂停诊断用儿科居家呼吸监测有效性及成本效益：研究理据、设计及方法学
Esther Oceja,Paula Rodríguez,María José Jurado et al.
Esther Oceja et al.
Obstructive sleep apnea (OSA) in children is a prevalent, albeit largely undiagnosed disease associated with a large spectrum of morbidities. Overnight in-lab polysomnography remains the gold standard diagnostic approach, but is time-consum...
Study Protocol of a Randomized Controlled Trial of Home Modification to Prevent Home Fall Injuries in Houses with Māori Occupants [0.03%]
毛利人居住房屋改造预防跌倒伤害的随机对照试验方案研究_protocol
Michael Keall,Hope Tupara,Nevil Pierse et al.
Michael Keall et al.
Worldwide, injuries due to falls in the home impose a substantial burden and merit considerable effort to find effective prevention measures. The current study is one of very few randomized controlled trials that assess the effectiveness of...
A Personalized Patient-Centered Intervention to Empower through Physical Activity the Patient in the Dialysis Center: Study Protocol for a Pragmatic Nonrandomized Clinical Trial [0.03%]
基于运动赋权的透析中心以患者为中心的干预措施的效果观察：实用非随机对照临床试验方案
Fabio Manfredini,Nicola Lamberti,Yuri Battaglia et al.
Fabio Manfredini et al.
Sedentariness of patients affected by end-stage kidney disease (ESKD) expose them to high risk of unfavorable clinical outcomes. Exercise training is effective in improving physical function, quality of life (QoL) and long-term outcomes. Ho...
The Impact of Diversified Farming Practices on Terrestrial Biodiversity Outcomes and Agricultural Yield Worldwide: A Systematic Review Protocol [0.03%]
全球多样化农业实践对陆地生物多样性成果和农业生产的影响：系统评价方案
Andrea C Sánchez,Natalia Estrada-Carmona,Stella D Juventia et al.
Andrea C Sánchez et al.
The expansion and intensification of agriculture have led to global declines in biodiversity. This paper presents a systematic review protocol to clarify under what management and landscape contexts diversified farming practices are effecti...