Mrinmoyee Majumder,Viswanathan Palanisamy
Mrinmoyee Majumder
Control of gene expression is critical in shaping the pro-and eukaryotic organisms' genotype and phenotype. The gene expression regulatory pathways solely rely on protein-protein and protein-nucleic acid interactions, which determine the fa...
Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data Files [0.03%]
评估协议对分析外周血单个核细胞流式细胞仪数据文件主观不确定度的影响
Rebecca Grant,Karen Coopman,Sandro Silva-Gomes et al.
Rebecca Grant et al.
Measured variability of product within Cell and Gene Therapy (CGT) manufacturing arises from numerous sources across pre-analytical to post-analytical phases of testing. Operators are a function of the manufacturing process and are an impor...
Validity and Efficacy of Methods to Define Blood Brain Barrier Integrity in Experimental Ischemic Strokes: A Comparison of Albumin Western Blot, IgG Western Blot and Albumin Immunofluorescence [0.03%]
检测缺血性卒中后血脑屏障损害方法的可靠性及有效性的比较：白蛋白Western blot、免疫荧光和IgG Western blot检测法
Maximilian Franke,Michael Bieber,Guido Stoll et al.
Maximilian Franke et al.
The clinical and preclinical research of ischemic strokes (IS) is becoming increasingly comprehensive, especially with the emerging evidence of complex thrombotic and inflammatory interactions. Within these, the blood brain barrier (BBB) pl...
Effectiveness of Low-Level Laser Therapy Associated with Strength Training in Knee Osteoarthritis: Protocol for a Randomized Placebo-Controlled Trial [0.03%]
低水平激光疗法联合力量训练治疗膝关节骨关节炎的有效性：随机对照试验方案
Martin Bjørn Stausholm,Ingvill Fjell Naterstad,Christian Couppé et al.
Martin Bjørn Stausholm et al.
Physical activity and low-level laser therapy (LLLT) can reduce knee osteoarthritis (KOA) inflammation. We are conducting a randomized placebo-controlled trial to investigate the long-term effectiveness of LLLT combined with strength traini...
Austin Bow
Austin Bow
The reduction in costs associated with performing RNA-sequencing has driven an increase in the application of this analytical technique; however, restrictive factors associated with this tool have now shifted from budgetary constraints to t...
RNA In Situ Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of Drosophila Species [0.03%]
果蝇类物种腹部和翅膀的原位杂交技术检测基因表达模式
Mujeeb Shittu,Tessa Steenwinkel,William Dion et al.
Mujeeb Shittu et al.
RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and n...
Inactivation and Elimination of SARS-CoV-2 in Biosamples Using Simple Fixatives and Ultrafiltration [0.03%]
使用简单固定剂和超滤法使生物样本中的SARS-CoV-2失活和消除
Ranjeet Kumar,Afsal Kolloli,Selvakumar Subbian
Ranjeet Kumar
The Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) causes Coronavirus disease-2019 (COVID-19), which is an ongoing pandemic that has significantly affected the health, economy, and socio-economic status of individuals worldwid...
A Modified Limiting Dilution Method for Monoclonal Stable Cell Line Selection Using a Real-Time Fluorescence Imaging System: A Practical Workflow and Advanced Applications [0.03%]
一种用于单克隆稳定细胞系选择的改良限稀法及其在实时荧光成像系统中的应用：工作流程与高级应用
Mingyu Ye,Martina Wilhelm,Ivaylo Gentschev et al.
Mingyu Ye et al.
Stable cell lines are widely used in laboratory research and pharmaceutical industry. They are mainly applied in recombinant protein and antibody productions, gene function studies, drug screens, toxicity assessments, and for cancer therapy...
Total Face Approach (TFA): A Novel 3D Approach to Describe the Main Cephalometric Craniomaxillofacial Parameters [0.03%]
全面部方法（TFA）：一种描述头影学颅面主要参数的新三维方法
Giovanna Perrotti,Giulia Baccaglione,Tommaso Clauser et al.
Giovanna Perrotti et al.
The aim of this study is to propose a 3D skeletal classification and relative normal values of reference. Method: from a pool of 271 cone-beam computerized tomography images 108 chin-summit examinations of the skull were selected and divide...
Concentration and Quantification of SARS-CoV-2 RNA in Wastewater Using Polyethylene Glycol-Based Concentration and qRT-PCR [0.03%]
基于聚乙二醇浓缩和qRT-PCR的SARS-CoV-2 RNA污水富集及定量研究
Kata Farkas,Luke S Hillary,Jamie Thorpe et al.
Kata Farkas et al.
Wastewater-based epidemiology has become an important tool for the surveillance of SARS-CoV-2 outbreaks. However, the detection of viruses in sewage is challenging and to date there is no standard method available which has been validated f...