In Vitro 3D Model of Human Endometrial Stromal and Trophoblast Cells: Techniques for an Optimized Formation and Cryopreservation of Spheroids [0.03%]
人子宫内膜基质和滋养细胞的体外三维模型：优化球形细胞系形成和冷冻保存的技术
Karthika Muthuraj,Iwona Scheliga,Dunja M Baston-Buest et al.
Karthika Muthuraj et al.
Three-dimensional (3D) cell culture models provide physiologically relevant systems that mimic the native endometrial environment better than 2D models and offer reliable platforms to study embryo implantation and maternal-embryo interactio...
A Protocol for Regulating Protein Liquid-Liquid Phase Separation Using NMR-Guided Mutagenesis [0.03%]
一种利用核磁共振指导的突变调节蛋白质液-液相分离的协议
Mayu Enomoto-Kusano,Kyoko Furuita,Takashi S Kodama et al.
Mayu Enomoto-Kusano et al.
Liquid-liquid phase separation (LLPS) underlies the formation of membraneless cellular compartments, yet experimental strategies that directly connect quantitative LLPS behavior with residue-level structural information remain limited. Here...
Eva Vargas,Inés de la Torre,Francisco J Esteban
Eva Vargas
We describe a comprehensive methodology for the application of game theory to omics data analysis, with a particular focus on coalitional games and Shapley values. This approach evaluates the cooperative distribution of genes within high-di...
Elías Hernández-Cruz,Lorena Jacqueline Gómez-Godínez,José Martín Ruvalcaba-Gómez et al.
Elías Hernández-Cruz et al.
Soil harbors the highest concentration of microorganisms in ecosystems, and their molecular characterization through high-throughput sequencing is essential for metagenomic studies. However, obtaining high-quality, high-concentration DNA is...
Development of an In Vitro Whole Blood Model to Study Anti-Inflammatory Effects of Strawberry Polyphenolic Compounds and Postprandial Inflammation [0.03%]
体外全血模型的发展，用于研究草莓多酚化合物的抗炎作用和餐后炎症反应
Skyelar Reuter,Peter Geevarghese Alex,Casey Weisfuss et al.
Skyelar Reuter et al.
Postprandial inflammation, characterized by elevated cytokines, is linked to metabolic diseases. Polyphenol-rich fruits like strawberries possess anti-inflammatory properties that may help reduce this response. Because clinical studies are ...
Improved Step-by-Step qPCR Method for Absolute Telomere Length Measurement [0.03%]
改进的一步法qPCR方法用于绝对端粒长度测定
Ekaterina Sergeevna Arshinova,Nataliia Sergeevna Karpova,Olga Leonidovna Terekhina et al.
Ekaterina Sergeevna Arshinova et al.
Telomere length is a crucial marker of cellular aging and genomic stability, with significant implications for age-related diseases and cancers. This study introduces an improved quantitative PCR (qPCR) method for measuring absolute telomer...
An Open-Source Automated Pipeline for Quantitative Morphological Analysis of 3D-Bioprinted Cancer Cell Spheroids [0.03%]
一种用于定量分析三维生物打印癌细胞球形结构的开源自动化流程
Pius N Amartey,Jocelyn S Kim,Yetunde I Kayode et al.
Pius N Amartey et al.
Three-dimensional (3D) culture systems that recapitulate the tumor microenvironment are essential for studying cancer cell behavior, drug response, and cell-matrix interactions. Here, we present a detailed protocol for generating 3D spheroi...
Comprehensive Analytical Profiling for Sustainable Jewelry: A Multi-Technique Characterization of Essential Oil-Modified Rosin [0.03%]
可持续珠宝的综合分析谱图：精油改性松香的多技术表征
Dantong Huang,Kaewbucha Manus,Apiwathnasorn Chalisa et al.
Dantong Huang et al.
Rosin, a renewable natural resin derived from pine trees, is a promising biomass material for sustainable product development, though its distinct intrinsic odor limits broader use. This study implemented a comprehensive analytical strategy...
A Novel Combined Soft Tissue and Bony Repair of Trochanteric Fractures in Revision Hip and Periprosthetic Fractures-Greater Trochanteric Abductor Tendon Augmentation (GTATA) [0.03%]
翻修髋关节和假体周围骨折股骨转子部骨折软组织及骨性联合修复的新方法-转子部臀肌腱加强（GTATA）术式
Nina Handzewniak,Abid Mahmood,Canan Metin et al.
Nina Handzewniak et al.
Introduction: Management of trochanteric fractures in revision hip surgery has a high incidence of non-union and complications. Fixation devices are often bulky, prone to breakage, and necessitate reoperation. This study ...
An Integrated Monitoring Protocol to Study the Effects of Management on the C Sequestration Potential of Mediterranean Pine Ecosystems [0.03%]
一个集成的监测方案，研究管理对地中海松林生态系统碳固存潜力的影响
Nikoleta Eleftheriadou,Efstathia D Mantzari,Natasa Kiorapostolou et al.
Nikoleta Eleftheriadou et al.
This article describes a field- and laboratory-based framework that can be used to monitor the C balance in Mediterranean pine forest ecosystems under different management practices that determine their structure and function. By jointly mo...