A Proximity Ligation Method to Detect Proteins Bound to Single-Stranded DNA after DNA End Resection at DNA Double-Strand Breaks [0.03%]
检测DNA双链断裂末端切除后与单链DNA结合的蛋白质的方法
Faith C Fowler,Jessica K Tyler
Faith C Fowler
After a DNA double-strand break, cells utilize either non-homologous end joining or homologous recombination to repair the broken DNA ends. Homologous recombination requires extensive nucleolytic processing of one of the DNA strands, result...
A Randomized Controlled Trial Protocol for Using an Accelerometer-Smartphone Application Intervention to Increase Physical Activity and Improve Health among Employees in a Military Workplace [0.03%]
军事工作场所员工使用加速度计-智能手机应用程序干预增加身体活动和改善健康的研究方案
Emilia Pietiläinen,Heikki Kyröläinen,Tommi Vasankari et al.
Emilia Pietiläinen et al.
Physical activity is beneficial for improving health and reducing sick leave absences. This article describes a protocol for an intervention using an interactive accelerometer smartphone application, telephone counselling, and physical acti...
Modern Approaches and Innovations on Methods and Imaging Protocols of the Maxillofacial District [0.03%]
现代颌面区方法和成像协议的创新及进展
Rodolfo Reda,Maurilio D&#x;Angelo,Alessio Zanza et al.
Rodolfo Reda et al.
In recent years, improvements in imaging techniques have profoundly changed the diagnosis of pathologies of the maxillofacial district [...].
Optimized Protocols for the Propagation and Quantification of Infectious Murine Hepatitis Virus (MHV-A59) Using NCTC Clone 1469 and 929 Cells [0.03%]
用于NCTC克隆1469和929细胞的鼠肝炎病毒（MHV-A59）增殖及感染量化的优化方法
Tautvydas Shuipys,Naim Montazeri
Tautvydas Shuipys
Murine hepatitis virus (MHV) is a non-human pathogen betacoronavirus that is evolutionarily and structurally related to the human pathogenic viruses SARS-CoV, MERS-CoV, and SARS-CoV-2. However, unlike the human SARS and MERS viruses, MHV re...
Protocol of Co-Culture of Human Osteoblasts and Osteoclasts to Test Biomaterials for Bone Tissue Engineering [0.03%]
人成骨细胞和破骨细胞共培养协议：用于组织工程骨移植材料的筛选及性能评估
Giorgia Borciani,Giorgia Montalbano,Nicola Baldini et al.
Giorgia Borciani et al.
New biomaterials and scaffolds for bone tissue engineering (BTE) applications require to be tested in a bone microenvironment reliable model. On this assumption, the in vitro laboratory protocols with bone cells represent worthy experimenta...
Improving the Quality of Patient Care and Healthcare Staff Well-Being through an Empathy Immersion Educational Programme in New Zealand: Protocol of a Feasibility and Pilot Study [0.03%]
通过同理心沉浸式教育项目改善新西兰患者护理和医护工作人员福祉的质量：一项可行性和试点研究的方案
Caz Hales,Chris K Deak,Tosin Popoola et al.
Caz Hales et al.
Empathy is positively related to healthcare workers and patients' wellbeing. There is, however, limited research on the effects of empathy education delivered in acute clinical settings and its impact on healthcare consumers. This research ...
Concurrent Measurement of Mitochondrial DNA Copy Number and ATP Concentration in Single Bovine Oocytes [0.03%]
单 bovine 卵母细胞线粒体DNA拷贝数和ATP浓度的同时测定
Casey C Read,Sadikshya Bhandari,Sarah E Moorey
Casey C Read
To sustain energy-demanding developmental processes, oocytes must accumulate adequate stores of metabolic substrates and mitochondrial numbers prior to the initiation of maturation. In the past, researchers have utilized pooled samples to s...
Diouri Lamia,Uwiringiyeyezu Théophile,Abdelouahab Hinde et al.
Diouri Lamia et al.
Ichthyoplankton is the cluster of planktonic organisms that consists of fish eggs and larvae. These planktonic stages belong to the temporary zooplankton, representing future exploitable stocks. The study of the early ontogenesis of fish pl...
Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse [0.03%]
从小鼠单个大脑中分离微胶质DNA和RNA的方法
Md Obayed Raihan,Brett A McGregor,Nathan A Velaris et al.
Md Obayed Raihan et al.
Microglia, the resident brain immune effectors cells, show dynamic activation level changes for most neuropsychiatric diseases, reflecting their complex regulatory function and potential as a therapeutic target. Emerging single-cell molecul...
Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage [0.03%]
扫描电镜和组织学方法在评估细胞工程构建效果以恢复透明软骨中的特殊性
Mikhail S Bozhokin,Svetlana A Bozhkova,Aleksandr A Rubel et al.
Mikhail S Bozhokin et al.
Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation...