Héctor Lorente-Martínez,Ainhoa Agorreta,Diego San Mauro
Héctor Lorente-Martínez
Molecular evolution analyses, such as detection of adaptive/purifying selection or ancestral protein reconstruction, typically require three inputs for a target gene (or gene family) in a particular group of organisms: sequence alignment, m...
Direct RT-qPCR Assay for the Detection of SARS-CoV-2 in Saliva Samples [0.03%]
用于检测唾液样本中SARS-CoV-2的直接RT-qPCR测定法
Francesco Saverio Tarantini,Siyu Wu,Harry Jenkins et al.
Francesco Saverio Tarantini et al.
Since mid-2020 there have been complexities and difficulties in the standardisation and administration of nasopharyngeal swabs. Coupled with the variable and/or poor accuracy of lateral flow devices, this has led to increased societal 'test...
Validation of In Vivo Nodal Assessment of Solid Malignancies with USPIO-Enhanced MRI: A Workflow Protocol [0.03%]
超小超顺磁性氧化铁纳米颗粒增强MRI技术的结节仿真和临床验证：工作流程协议
Daphne A J J Driessen,Didi J J M de Gouw,Rutger C H Stijns et al.
Daphne A J J Driessen et al.
Background: In various cancer types, the first step towards extended metastatic disease is the presence of lymph node metastases. Imaging methods with sufficient diagnostic accuracy are required to personalize treatment. ...
Evaluating the Co-design of an Age-Friendly, Rural, Multidisciplinary Primary Care Model: A Study Protocol [0.03%]
一种老年友好型农村多学科初级保健模型的协同设计评价：研究方案
Rachel Winterton,Kathleen Brasher,Mark Ashcroft
Rachel Winterton
In the context of increased rates of frailty and chronic disease among older people, there is a need to develop age-friendly, integrated primary care models that place the older person at the centre of their care. However, there is little e...
Assessment of Common Risk Factors of Non-Communicable Diseases (NCDs) and Periodontal Disease in Indian Adults: An Analytical Cross-Sectional Study [0.03%]
评估印度成年人常见非传染性疾病(NCDs)和牙周病风险因素的分析横断面研究
Lakshmi Puzhankara,Chandrashekar Janakiram
Lakshmi Puzhankara
Risk factors that predispose individuals towards major non-communicable diseases (NCDs) and periodontal disease (PD) often co-occur in the same individual. The common risk factor approach (CRFA) for controlling the risk factors associated w...
Retinoblastoma Cell Growth In Vitro and Tumor Formation In Ovo-Influence of Different Culture Conditions [0.03%]
不同培养条件对视网膜母细胞瘤细胞生长及肿瘤形成的影响
Annika Doege,Rebecca Steens,Nicole Dünker et al.
Annika Doege et al.
Retinoblastoma (RB) is a primary intraocular malignancy in childhood. Relapses may develop and cause secondary cancers during later development. This study was set up to identify optimal cell culture conditions for RB cell growth in vitro a...
Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential Implications for Children-Related Diseases [0.03%]
小样本外周血单个核细胞分离方法优化及儿童疾病研究中的应用探索
Deanira Patrone,Nicola Alessio,Nicola Antonucci et al.
Deanira Patrone et al.
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an...
Quantitative Studies on the Interaction between Saposin-like Proteins and Synthetic Lipid Membranes [0.03%]
类脂蛋白与合成脂质膜相互作用的定量研究
Suzanne I Sandin,Eva de Alba
Suzanne I Sandin
Members of the saposin-fold protein family and related proteins sharing a similar fold (saposin-like proteins; SAPLIP) are peripheral-membrane binding proteins that perform essential cellular functions. Saposins and SAPLIPs are abundant in ...
Harnessing Intronic microRNA Structures to Improve Tolerance and Expression of shRNAs in Animal Cells [0.03%]
利用内含子中的microRNA结构提高动物细胞中shRNA的耐受性和表达水平
Arjun Challagulla,Mark L Tizard,Timothy J Doran et al.
Arjun Challagulla et al.
Exogenous RNA polymerase III (pol III) promoters are commonly used to express short hairpin RNA (shRNA). Previous studies have indicated that expression of shRNAs using standard pol III promoters can cause toxicity in vivo due to saturation...
Radoslaw Bednarek
Radoslaw Bednarek
Cell monolayers, including endothelial and epithelial cells, play crucial roles in regulating the transport of biomolecules to underlying tissues and structures via intercellular junctions. Moreover, the monolayers form a semipermeable barr...