Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation [0.03%]
基于流式细胞术和RSM的微藻SYTO9/SYBR Green I核染优化方案：实验设计、影响因素及验证
Yob Ihadjadene,Thomas Walther,Felix Krujatz
Yob Ihadjadene
Multiple fluorochromes are extensively used to investigate different microalgal aspects, such as viability and physiology. Some of them can be used to stain nucleic acids (DNA). Well-known examples are SYBR Green I and SYTO 9, the latter of...
Rationale and Protocol of the Multimodality Evaluation of Antibody-Mediated Injury in Heart Transplantation (LEONE-HT) Observational Cross-Sectional Study [0.03%]
评价心脏移植受者抗体介导损伤的多模态评估（LEONE-HT）观察性横断面研究的设计与方法
Jorge Nuche,Javier de la Cruz Bertolo,Irene Marco Clement et al.
Jorge Nuche et al.
Introduction: Heart transplant (HT) survival has barely improved in the last decades, which is unsatisfactory for many HT recipients. The development of anti-human leukocyte antigen (anti-HLA) antibodies in HT patients is...
Quantitative Assessment of Histone H2B Monoubiquitination in Yeast Using Immunoblotting [0.03%]
利用免疫印迹法对酵母组蛋白H2B单泛素化的定量评估
Andrew M Leng,Kaitlin S Radmall,Prakash K Shukla et al.
Andrew M Leng et al.
Studies in Saccharomyces cerevisiae and Schizosaccharomyces pombe have enhanced our understanding of the regulation and functions of histone H2B monoubiquitination (H2Bub1), a key epigenetic marker with important roles in transcription and ...
Performance Comparison of a Duplex Implementation of the CDC EUA 2019-nCoV Assay with the Seegene Allplex-SARS-CoV-2 Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Swab Samples [0.03%]
CDC EUA 2019-nCoV检测试剂盒双管法与Seegene Allplex-SARS-CoV-2试剂盒咽拭子样本检测性能比较研究
Karen Marcela Jiménez,Dora Janeth Fonseca-Mendoza,Adrien Morel et al.
Karen Marcela Jiménez et al.
RT-PCR tests have become the gold standard for detecting the SARS-CoV-2 virus in the context of the COVID-19 pandemic. Because of the extreme number of cases in periodic waves of infection, there is a severe financial and logistical strain ...
Damilola Caleb Akintayo,Srinivasa Rao Manne,Beatriz G de la Torre et al.
Damilola Caleb Akintayo et al.
Herein we report a practical approach for peptide synthesis using second-generation fibrous polyacrylamide resin (Li-resin, "Li" is coming from the name of its inventor, Yongfu Li). This resin with the corresponding handle was used for soli...
Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues [0.03%]
单染及多重免疫组化在大鼠和猪组织中检测血小板和中性粒细胞的方法
Stephanie Arnold,Sarah Watts,Emrys Kirkman et al.
Stephanie Arnold et al.
Platelet-neutrophil complexes (PNCs) occur during the inflammatory response to trauma and infections, and their interactions enable cell activation that can lead to tissue destruction. The ability to identify the accumulation and tissue loc...
Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune Cells [0.03%]
基于多参数流式细胞术的小鼠肝免疫细胞免疫表型分析
Lenka Vanekova,Marketa Pimkova Polidarova,Vaclav Veverka et al.
Lenka Vanekova et al.
The liver is a complex organ that governs many types of metabolisms, including energy metabolism and other cellular processes. The liver also plays a crucial role in important functions in immunity, and the activity of liver tissue-associat...
A Genotype-Independent, Simple, Effective and Efficient in Planta Agrobacterium-Mediated Genetic Transformation Protocol [0.03%]
一种独立于基因型的、简便有效的植物农杆菌介导遗传转化 protocol
Pushpa Kharb,Rinku Chaudhary,Narendra Tuteja et al.
Pushpa Kharb et al.
Crop improvement under changing climatic conditions is required to feed the growing global population. The development of transgenic crops is an attractive and conceivably the most effective approach for crop improvement with desired traits...
In Vitro Screening Method for Characterization of Macrophage Activation Responses [0.03%]
体外巨噬细胞活化反应特征分析的筛选方法
Brandon W Lewis,Sonika Patial,Yogesh Saini
Brandon W Lewis
Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous or exogenous stimuli. Due to the existence of limitless stimuli and a multitude of receptors on macrophage surfaces, the nature of activatio...
Laser Microdissection-Mediated Isolation of Butterfly Wing Tissue for Spatial Transcriptomics [0.03%]
激光显微解剖介导的蝴蝶翅膀组织空间转录组学分离
Tirtha Das Banerjee,Shen Tian,Antόnia Monteiro
Tirtha Das Banerjee
The assignment of specific patterns of gene expression to specific cells in a complex tissue facilitates the connection between genotype and phenotype. Single-cell sequencing of whole tissues produces single-cell transcript resolution but l...