Beatriz G de la Torre,Fernando Albericio
Beatriz G de la Torre
According to the Food and Drug Administration (FDA), there are two kinds of drugs, namely New Chemical Entities (NCEs) and Biologics [...].
Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins [0.03%]
优化细胞外蛋白质合成以提高色霉素的产量和活性
Xing Jin,Weston Kightlinger,Seok Hoon Hong
Xing Jin
Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it ...
Improving the Quality of Maternity Care through the Introduction of Professional Midwives and Mentoring in Selected Sub-District Hospitals in Bangladesh: A Mixed Method Study Protocol [0.03%]
通过引进专业助产士和指导改善孟加拉国选定的区级医院孕产妇护理质量：一项混合方法研究方案
Rondi Anderson,Sojib Bin Zaman
Rondi Anderson
Introduction: Bangladesh introduced professional midwives in 2018 to address gaps in sexual and reproductive health services, focusing on improved maternity care. Facility mentoring has been introduced in selected facilities within the gove...
Fostering Need-Supportive Behaviors in Physical Education Teachers and Parents: A Cluster Randomized Controlled Trial Study Protocol of a Web-Based Intervention on Secondary School Students' Physical Activity [0.03%]
促进体育教师和家长支持学生成长行为：基于网络的一站式干预鼓励中学生身体活动随机对照试验研究方案
Pille-Riin Meerits,Henri Tilga,Andre Koka
Pille-Riin Meerits
Despite various benefits of physical activity, children are increasingly inactive. Both school physical education classes and support from parents are important determinants of physical activity level of children and adolescents. We aim to ...
Solid-Phase Microextraction-Gas Chromatography Analytical Strategies for Pesticide Analysis [0.03%]
固体相微萃取-气相色谱农药分析方法学研究进展
Juan Aspromonte,Carlina Lancioni,Giorgia Purcaro
Juan Aspromonte
Due to their extensive use and the globalized commerce of agricultural goods, pesticides have become a global concern. Despite the undoubtful advantages of their use in agricultural practices, their misuse is a threat to the environment and...
Inexpensive, Accurate, and Stable Method to Quantitate Blood Alanine Aminotransferase (ALT) Levels [0.03%]
一种测定血液丙氨酸氨基转移酶（ALT）水平的低成本、精确和稳定的方法
Phillipp Hartmann,Bernd Schnabl
Phillipp Hartmann
Alanine aminotransferase (ALT) levels are frequently determined in serum and plasma samples and are a primary measure to quantitate hepatocellular injury in rodents, humans, and other organisms. An accurate, reliable, and scalable assay is ...
Neuroimaging through Sonolucent Cranioplasty: A Systematic Scoping Review Protocol [0.03%]
颅骨塑形术中超声透明的神经影像学：一项系统综述协议
Christina P Rossitto,Alex Devarajan,Gabrielle Price et al.
Christina P Rossitto et al.
Cranioplasty is a neurosurgical procedure in which the skull bone is repaired after craniectomy. Recently, studies have suggested that sonolucent synthetic materials are safe and useful for cranioplasty. Sonolucent cranioplasty (SC) implant...
A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9 [0.03%]
用于酿酒酵母CRISPR-Cas9无疤痕基因组编辑的简便改进方法
Rhiannon R Aguilar,Zih-Jie Shen,Jessica K Tyler
Rhiannon R Aguilar
Until recently, the favored method for making directed modifications to the budding yeast genome involved the introduction of a DNA template carrying the desired genetic changes along with a selectable marker, flanked by homology arms. This...
Radiogenomics, Breast Cancer Diagnosis and Characterization: Current Status and Future Directions [0.03%]
放射基因组学在乳腺癌诊断和分类中的现状及未来方向
Francesca Gallivanone,Gloria Bertoli,Danilo Porro
Francesca Gallivanone
Breast cancer (BC) is a heterogeneous disease, affecting millions of women every year. Early diagnosis is crucial to increasing survival. The clinical workup of BC diagnosis involves diagnostic imaging and bioptic characterization. In recen...
Sequential Isolation of Microglia and Astrocytes from Young and Aged Adult Mouse Brains for Downstream Transcriptomic Analysis [0.03%]
从小鼠大脑中分离微胶质细胞和 astrocyte 进行下游转录组分析
Ruchelle G Buenaventura,Alex C Harvey,Mark P Burns et al.
Ruchelle G Buenaventura et al.
In aging, the brain is more vulnerable to injury and neurodegenerative disease, but the mechanisms responsible are largely unknown. Evidence now suggests that neuroinflammation, mediated by resident brain astrocyte and microglia populations...