Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids [0.03%]
高效凝胶电泳及难处理质粒简便筛选的可持续方法学研究
Nadeem Asad,Emily Smith,Sudeep Shakya et al.
Nadeem Asad et al.
We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of d...
Dmitry Penkov,Ekaterina Zubkova,Yelena Parfyonova
Dmitry Penkov
Tn5 transposase use in biotechnology has substantially advanced the sequencing applications of genome-wide analysis of cells. This is mainly due to the ability of Tn5 transposase to efficiently transpose DNA essentially randomly into any ta...
Neha Nanajkar,Lekhana S Mruthyunjaya,Deepesh Nagarajan
Neha Nanajkar
Understanding the mechanisms and kinetics of membrane damage is of interest to researchers in several overlapping fields of biology. In this study, we describe the development and validation of a simple 32PO43- release radioassay used to tr...
Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury [0.03%]
系统鉴定最佳管家基因 准确进行复杂创伤后组织转录组和蛋白质组分析
Andrea H Dragon,Cassie J Rowe,Alisha M Rhodes et al.
Andrea H Dragon et al.
Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ bet...
Nadeem Asad,Scott Cregg,Sudeep Shakya et al.
Nadeem Asad et al.
A common laboratory method involves gel electrophoresis followed by photographic documentation of the results, a procedure which is performed worldwide by students and experienced scientists alike. Proprietary Gel Documentation Systems are ...
On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging [0.03%]
化学发光成像现场检测绿色咖啡中绿原酸含量
Sergi Mallorca-Cebria,Yolanda Moliner-Martinez,Carmen Molins-Legua et al.
Sergi Mallorca-Cebria et al.
The potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, qualit...
Augmenting Mental Health Support for Patients Accessing Different Degrees of Formal Psychiatric Care through a Supportive Text Messaging Program: Protocol for a Randomized Controlled Trial [0.03%]
通过支持性文本消息程序增强不同层次正式精神卫生护理患者的精神健康支持：一项随机对照试验方案
Raquel da Luz Dias,Reham Shalaby,Belinda Agyapong et al.
Raquel da Luz Dias et al.
Patients feel more vulnerable when accessing community mental health programs for the first time or after being discharged from psychiatric inpatient units. Long wait times for follow-up appointments, shortage of mental health professionals...
Mastering DNA Content Estimation by Flow Cytometry as an Efficient Tool for Plant Breeding and Biodiversity Research [0.03%]
流式细胞仪测定DNA含量作为植物育种和生物多样性研究的有效工具及其应用原理要点
Maria Fomicheva,Elena Domblides
Maria Fomicheva
Flow cytometry gives a unique opportunity to analyze thousands of individual cells for multiple parameters in a course of minutes. The most commonly used flow cytometry application in plant biology is estimation of nuclear DNA content. This...
Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Leaf and Bark Samples of Sambucus nigra Using High-Performance Liquid Chromatography (HPLC) [0.03%]
高效液相色谱法测定桑树叶片和树皮中多环芳烃(PAHs)的方法
Fausto Viteri,Nazly E Sánchez,Katiuska Alexandrino
Fausto Viteri
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous organic compounds coming from natural or anthropogenic activities. Tree organs such as leaves and barks have been used to monitor urban air quality and have achieved remarkable ecologic...
Tatyana A Belashova,Anna A Valina,Evgeniy I Sysoev et al.
Tatyana A Belashova et al.
Amyloids are fibrillar proteins with a cross-β structure. Pathological amyloids are associated with the development of a number of incurable diseases, while functional amyloids regulate vital processes. The detection of unknown amyloids in...