Ana Salomé Pires,Sveva Bollini,Maria Filomena Botelho et al.
Ana Salomé Pires et al.
The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA1...
A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis [0.03%]
一种用于微孔板上MBP融合蛋白固定的成本效益方法及其在简便蛋白质相互作用分析中的应用：基于明胶淀粉-琼脂糖混合物的方法
Yuri Emoto,Ryoya Katayama,Emi Hibino et al.
Yuri Emoto et al.
The detection and quantification of protein-protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase...
Comparison of Automated and Traditional Western Blotting Methods [0.03%]
自动 western 印迹法与传统 western 印迹法的比较研究进展
Aino Sormunen,Emma Koivulehto,Kari Alitalo et al.
Aino Sormunen et al.
Traditional Western blotting is one of the most used analytical techniques in biological research. However, it can be time-consuming and suffer from a lack of reproducibility. Consequently, devices with different degrees of automation have ...
A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable Haemophilus influenzae [0.03%]
一种用于从非典型流感嗜血杆菌分离外膜囊泡的稳健型方案
M Daben J Libardo,Eberhard Durr,Lorraine D Hernandez
M Daben J Libardo
Outer membrane vesicles (OMVs) are lipid structures containing various biomolecules in their native environment and are spontaneously shed by gram-negative bacteria. OMVs perform several biological functions critical to both bacterial physi...
Intrarater Reliability and Analysis of Learning Effects in the Y Balance Test [0.03%]
Y平衡试验的阅卷者一致性及学习效应分析
Olli Kattilakoski,Noora Kauranen,Mari Leppänen et al.
Olli Kattilakoski et al.
While the general reliability of the Y balance test has been previously found to be excellent, earlier reviews highlighted a need for a more consistent methodology between studies. The purpose of this test-retest intrarater reliability stud...
A Multiparametric Protocol for the Detailed Phytochemical and Antioxidant Characterisation of Plant Extracts [0.03%]
植物提取物的详细植化和抗氧化特征分析的多参数协议
Anna Michalaki,Konstantinos Grintzalis
Anna Michalaki
Medicinal and herbal plants are abundant sources of phytochemicals, which are biologically active compounds with potential health benefits. The characterisation of phytochemicals has been the subject of many studies, but there is a lack of ...
Acceleration of CRISPR/Cas9-Mediated Editing at Multiple Sites in the Saccharomyces cerevisiae Genome [0.03%]
加速CRISPR / Cas9在酿酒酵母基因组多个位点的编辑
Alexey D Karpukhin,Fanis A Sabirzyanov,Vsevolod A Serebrianyi
Alexey D Karpukhin
The application of the CRISPR/Cas9-based genome editing technique to the yeast Saccharomyces cerevisiae has made it possible to simultaneously modify several sites, particularly to integrate several expression cassettes. The existing method...
Critical Evaluation of Embedding Media for Histological Studies of Early Stages of Chick Embryo Development [0.03%]
鸡胚胎早期发育组织学研究中包埋介质的选择评价
Melyssa Kmecick,Mariliza Cristine Vieira da Costa,Eduardo da Costa Ferreira et al.
Melyssa Kmecick et al.
A histological examination is an important tool in embryology, developmental biology, and correlated areas. Despite the amount of information available about tissue embedding and different media, there is a lack of information regarding bes...
Serological Cross-Reaction between Six Thiadiazine by Indirect ELISA Test and Their Antimicrobial Activity [0.03%]
六种噻二嗪的间接ELISA血清学交叉反应及抑菌活性测定
Mishell Ortiz,Hortensia Rodríguez,Elisabetta Lucci et al.
Mishell Ortiz et al.
Malaria is a parasitic infection caused by a protozoon of the genus Plasmodium, transmitted to humans by female biting mosquitoes of the genus Anopheles. Chloroquine and its derivates have caused the parasite to develop drug resistance in e...
Established and Emerging Methods for Protecting Linear DNA in Cell-Free Expression Systems [0.03%]
细胞自由表达体系中保护线性DNA的成熟和新兴方法
Trevor J Fochtman,Javin P Oza
Trevor J Fochtman
Cell-free protein synthesis (CFPS) is a method utilized for producing proteins without the limits of cell viability. The plug-and-play utility of CFPS is a key advantage over traditional plasmid-based expression systems and is foundational ...