On-Slide Lambda Protein Phosphatase-Mediated Dephosphorylation of Fixed Samples [0.03%]
固定样本的滑动去磷酸化蛋白质磷酸酶介导的脱磷酸化
Alexander Tishchenko,Cliff Van Waesberghe,Herman W Favoreel
Alexander Tishchenko
Protein phosphorylation is a ubiquitous post-translational modification that regulates a plethora of intracellular processes, making its analysis crucial for understanding intracellular dynamics. The commonly used methods, such as radioacti...
A Modified Protocol for the Isolation, Culture, and Characterization of Human Smooth Muscle Cells from the Umbilical Cord [0.03%]
人脐带平滑肌细胞的分离、培养和表征的改良方案
Asmaa Q Ibrahim,Mohammed S Abdullah,Mamoun Ahram et al.
Asmaa Q Ibrahim et al.
Background: Vascular smooth muscle cells (VSMCs) and vascular endothelial cells are key participants in the pathogenesis of atherosclerosis. Human umbilical vein endothelial cells (HUVECs) and VSMCs are useful models to d...
An Improved Technique for Genotyping the ABCB1 Gene Variant of Exon 21 [0.03%]
一种改进的ABCB1基因第21外显子多态性分型方法
Johanna Romina Zuccoli,Priscila Ayelén Pagnotta,Viviana Alicia Melito et al.
Johanna Romina Zuccoli et al.
The Multidrug Resistance protein (ABCB1, MDR1) is involved in the transport of xenobiotics and antiretroviral drugs. Some variants of the ABCB1 gene are of clinical importance; among them, exon 12 (c.1236C>T, rs1128503), 21 (c.2677G>T/A, rs...
Non-Antibiotic Prophylaxis for Recurrent UTIs in Neurogenic Lower Urinary Tract Dysfunction (NAPRUN): Study Protocol for a Prospective, Longitudinal Multi-Arm Observational Study [0.03%]
用于神经源性下尿路功能障碍反复发作的尿路感染的非抗生素预防（NAPRUN）：前瞻性纵向多臂观察研究方案
Fabian P Stangl,Laila Schneidewind,Bernhard Kiss et al.
Fabian P Stangl et al.
Introduction: Patients with neurogenic lower urinary tract dysfunction (NLUTD) reliant on intermittent self-catheterization for bladder emptying are at an increased risk of recurrent urinary tract infections (rUTI). So fa...
The Streaming Web-Based Exercise at Home Study for Breast and Prostate Cancer Survivors: A Feasibility Study Protocol [0.03%]
面向乳腺癌和前列腺癌幸存者基于流媒体的居家锻炼研究可行性研究方案
Celina H Shirazipour,Rachel M Ruggieri-Bacani,Laura Lockshon et al.
Celina H Shirazipour et al.
Background: Despite the known benefits of physical activity in cancer survivors, adherence to exercise guidelines remains low. Known barriers to adhering to guidelines include a lack of time and an unwillingness to return...
Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells [0.03%]
猪原代角膜细胞的分离及培养方法
Alice Rocha Teixeira Netto,Marc Dieter Hrusa,Karl-Ulrich Bartz-Schmidt et al.
Alice Rocha Teixeira Netto et al.
In ophthalmic research, there is a strong need for in vitro corneal cell models. Here, we describe different protocols for the cultivation of primary corneal cells that were isolated from porcine eyes. This primary cell culture can be used ...
Radiation Exposure in Endovascular Surgery According to Complexity: Protocol for a Prospective Observational Study [0.03%]
根据复杂程度的血管内手术中的辐射暴露：前瞻性观察性研究方案
Dorelly Tanayra Martinez Del Carmen,Pablo Saldaña Gutierrez,Ramon Vila Coll et al.
Dorelly Tanayra Martinez Del Carmen et al.
In the past decades, we have witnessed tremendous developments in endovascular surgery. Nowadays, highly complex procedures are performed by minimally invasive means. A key point is equipment improvement. Modern C-arms provide advanced imag...
The Impact of Introducing Midwives and also Mentoring on the Quality of Sexual, Reproductive, Maternal, Newborn, and Adolescent Health Services in Low- and Middle-Income Countries: An Integrative Review Protocol [0.03%]
关于助产士和指导计划对低收入和中等收入国家的性与生殖健康及权利(SRHR)服务的影响：综合述评方案
Rondi Anderson,Sojib Bin Zaman,Mark Limmer
Rondi Anderson
Introduction: Midwives have the potential to significantly contribute to health-delivery systems by providing sexual, reproductive, maternal, newborn, and adolescent health (SRMNAH) care. However, scant research finds bar...
Creating Implicit Measure Stimulus Sets Using a Multi-Step Piloting Method [0.03%]
使用多阶段试测方法创建隐含衡量刺激对象群
Daniel J Phipps,Kyra Hamilton
Daniel J Phipps
The effect of arbitrary stimulus selection is a persistent concern when employing implicit measures. The current study tests a data-driven multi-step procedure to create stimulus items using a combination of free-recall and survey data. Six...
Automated Dashboards for the Identification of Pathogenic Circulating Tumor DNA Mutations in Longitudinal Blood Draws of Cancer Patients [0.03%]
用于识别癌症患者纵向血标本中致病性循环肿瘤DNA突变的自动化仪表板
Aleksandr Udalov,Lexman Kumar,Anna N Gaudette et al.
Aleksandr Udalov et al.
The longitudinal monitoring of patient circulating tumor DNA (ctDNA) provides a powerful method for tracking the progression, remission, and recurrence of several types of cancer. Often, clinical and research approaches involve the manual r...