Utilizing Dichloromethane as an Extremely Proficient Substitute for Phenol/Chloroform in Extracting RNA with Exceptional Purity from Woody Tissues of Coconut [0.03%]
采用二氯甲烷替代酚/氯仿从椰子木质组织中提取高纯度RNA的研究
Amjad Iqbal,Yaodong Yang
Amjad Iqbal
Procuring high-grade RNA from mature coconut tissues is a tricky and labor-intensive process due to the intricate scaffold of polysaccharides, polyphenols, lipids, and proteins that form firm complexes with nucleic acids. However, we have e...
Protocol Study: Resistance Training Program, Nutritional, Sleep, and Screen Use Recommendations in Schoolchildren from Educational Centers in the Extreme South of Chile [0.03%]
基于智利极端南部教育机构的学校儿童抗阻训练计划、营养、睡眠和屏幕使用推荐研究
Javier Albornoz-Guerrero,Olga Barceló,Sonia García-Merino et al.
Javier Albornoz-Guerrero et al.
Background: Childhood obesity has tripled, reaching critical levels of malnutrition. This factor is directly associated with a poorer health-related quality of life of the child and adolescent population. This article pre...
Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation [0.03%]
一种非洲猪瘟病毒分离的新型猪骨髓原代细胞培养物制备方案
Olga Puzankova,Vera Gavrilova,Roman Chernyshev et al.
Olga Puzankova et al.
Isolation of African swine fever virus (ASFV) is a critical step towards the identification, titration, characterization, and even modification of the virus. Therefore, it is important to identify a suitable cell line that supports the effi...
Real-Time Monitoring of Lysosomal Membrane Permeabilization Using Acridine Orange [0.03%]
应用吖啶橙实时监测溶酶体膜去极化现象
Ida Eriksson,Linda Vainikka,Hans Lennart Persson et al.
Ida Eriksson et al.
Loss of lysosomal membrane integrity results in leakage of lysosomal hydrolases to the cytosol which might harm cell function and induce cell death. Destabilization of lysosomes often precede apoptotic or necrotic cell death and occur durin...
Comparative Analysis of Primers Used for 16S rRNA Gene Sequencing in Oral Microbiome Studies [0.03%]
口腔微生物组研究中用于16S rRNA基因测序引物的比较分析
Hee Sam Na,Yuri Song,Yeuni Yu et al.
Hee Sam Na et al.
Recent advances in genomic technologies have enabled more in-depth study of the oral microbiome. In this study, we compared the amplicons generated by primers targeting different sites of the 16S rRNA gene found in the Human Oral Microbiome...
Application of a Customised Franz-Type Cell Coupled with HPTLC to Monitor the Timed Release of Bioactive Components in Complex Honey Matrices [0.03%]
改良Franz扩散池结合HPTLC监测复杂蜂蜜基质中活性成分定时释放的应用
Md Lokman Hossain,Minh Nguyen,Leah Benington et al.
Md Lokman Hossain et al.
The aim of this study was to assess the release profile of components in five different honeys (a New Zealand Manuka and two Western Australian honeys, a Jarrah honey and a Coastal Peppermint honey) and their corresponding honey-loaded gel ...
Ultrasound-Assisted Anthocyanins Extraction from Pigmented Corn: Optimization Using Response Surface Methodology [0.03%]
响应面法优化超声辅助色素玉米中花色苷的提取
Annisa Nurkhasanah,Titouan Fardad,Ceferino Carrera et al.
Annisa Nurkhasanah et al.
This study aimed to determine the optimal UAE conditions for extracting anthocyanins from pigmented corn using the Box-Behnken design (BBD). Six anthocyanins were identified in the samples and were used as response variables to evaluate the...
Geriatric Oncology in the Instagram Era: Feasibility and Acceptability Randomised Controlled Trial on Adopting PhotoVoice to Enable Empowerment, Patient-Centred Care, and Shared Decision Making-Study Protocol [0.03%]
Instagram时代的姑息肿瘤学：采用PhotoVoice进行赋权、以患者为中心的护理和共同决策的可行性和可接受性随机对照试验-研究方案
Christopher Steer,Tshepo Rasekaba,Kylie Owen et al.
Christopher Steer et al.
Geriatric assessment (GA) is fundamental to optimising cancer care in older adults, yet implementing comprehensive GA tools in real-world clinical settings remains a challenge. This study aims to assess the feasibility and acceptability of ...
Variations in Microcirculatory and Hemodynamic Parameters during Oncological Demolitive-Reconstructive Head and Neck Surgery: A Protocol for an Observational Study [0.03%]
头颈肿瘤毁损重建手术中微循环和血流动力学参数的变化：一项观察性研究的方法学报告
Chiara Adembri,Andrea Ungar,Iacopo Cappellini et al.
Chiara Adembri et al.
(1) Background: Oncological demolitive-reconstructive surgeries in the head and neck region cause significant stress on patients' biohumoural, cardiac, and vascular systems, leading to disturbances in macrocirculatory and microcirculatory p...
Ex Vivo Perfusion of Porcine Pancreas and Liver Sourced from Commercial Abattoirs after Circulatory Death as a Research Resource: A Methodological Study [0.03%]
取自商业屠宰场的猪胰腺和肝脏器官的体外灌注研究：一项方法学研究
Zainab L Rai,Morenike Magbagbeola,Katie Doyle et al.
Zainab L Rai et al.
Background: Machine perfusion (MP) is increasingly used for human transplant organ preservation. The use of MP for research purposes is another opportunity for this technology. The porcine pancreas and liver are similar i...