Optimized Protocol for Primary Rat Hepatocyte Isolation and a Model for Investigating Experimental Steatosis [0.03%]
大鼠原代肝细胞优化分离方案及实验性肝脂变模型的建立
Amani A Harb,Mohammad AlSalem,Shtaywy Abdalla
Amani A Harb
Background: Primary hepatocytes are excellent models for studying liver functions and liver diseases. However, obtaining high yields of viable hepatocytes remains technically challenging, limiting their broader applicatio...
A Comparative Bioinformatic Analysis of Optic Nerve Axon Regeneration Lipidomes Using the Xenopus laevis as a Model System [0.03%]
采用非洲爪蟾模型系统的视神经轴突再生脂质组比较生物信息学分析
Vernon S Volante,Fiona L Watson,Sanjoy K Bhattacharya
Vernon S Volante
Lipidomics is a rapidly growing branch of metabolomics that identifies lipid compositions of samples to learn more about disease and identify potential novel therapeutic targets. In the context of ophthalmology, lipidomic research has incre...
Hina Jhelum,Benedikt B Kaufer,Joachim Denner
Hina Jhelum
Xenotransplantation using pig cells, tissues, or organs is advancing toward clinical application to address the shortage of human donor organs for treating organ failure. However, this emerging technology carries the risk of transmitting pa...
Luca Legato,Matteo Bisio,Filippo Fasano et al.
Luca Legato et al.
In the last few decades, chimeric antigen receptor (CAR) T-cell therapy has led to a paradigm shift in the treatment of hematological malignancies, including various subtypes of B-cell non-Hodgkin's lymphoma, B-cell acute lymphoblastic leuk...
Compendium of Agrobacterium-Mediated Tissue Culture Transformation Methods of Various Solanaceae Species [0.03%]
农杆菌介导的各种茄科物种组织培养转化方法汇编
Caterina Brancato,Najmeh Heusch,Kenneth Wayne Berendzen
Caterina Brancato
The routine transformation and genetic modification of many plant species other than Arabidopsis thaliana is still an arduous task. In many cases, it is necessary to use special tissues or conditions performed under sterile tissue culture c...
A Rapid LC-MS/MS Method for Quantification of Biogenic Amines in Meat: Validation and Application for Food Safety Monitoring [0.03%]
肉制品中生物胺的定量检测方法及其在食品安全监测中的应用
Giulia Rampazzo,Giacomo Depau,Giampiero Pagliuca et al.
Giulia Rampazzo et al.
Biogenic amines (BAs) are nitrogenous compounds naturally present in protein-rich foods, whose accumulation may indicate spoilage and pose health risks. This study presents the development and validation of a rapid LC-MS/MS method for the s...
Creation of a Novel Coding Program to Identify Genes Controlled by miRNAs During Human Rhinovirus Infection [0.03%]
创建新型编码程序以识别人鼻病毒感染期间由miRNA控制的基因
Pax Bosner,Emily Smith,Victoria Cappleman et al.
Pax Bosner et al.
Human rhinovirus (RV) is the most frequent cause of the common cold, as well as severe exacerbations of chronic obstructive pulmonary disease (COPD) and asthma. Currently, there are no effective and accurate diagnostic tools or antiviral th...
A Safe and Accessible Cell-Based Spike-ACE2 Binding Assay for Evaluating SARS-CoV-2 Neutralization Activity in Biological Samples Using Flow Cytometry [0.03%]
基于细胞的Spike-ACE2结合试验评估生物样本中的SARS-CoV-2中和活性：使用流式细胞仪进行安全且可访问的实验
Martin A Rossotti,Shannon Ryan,Greg Hussack et al.
Martin A Rossotti et al.
SARS-CoV-2, the agent responsible for coronavirus disease in 2019 (COVID-19), has caused extensive global health and socioeconomic impact due to its transmissibility and pathology. As a result, it was classified as a Risk Group 3 human path...
Recombinant Expression and Purification of the Cyanobacterial Chaperone HtpG from Synechococcus elongatus PCC 7942 [0.03%]
来自Synechococcus elongatus PCC 7942的重组热休克蛋白HtpG的表达和纯化
Liqun Jiang,Ibrahim D Boyenle,Nicolas Delaeter et al.
Liqun Jiang et al.
The 90 kDa Heat Shock Protein (Hsp90) is an essential and highly conserved molecular chaperone that supports the folding and maturation of a diverse array of client proteins across prokaryotic and eukaryotic organisms. In bacteria, HtpG, th...
A Scalable Protocol for Ex Vivo Production of CAR-Engineered Human NK Cells [0.03%]
一种可扩展的离体生产工程化人NK细胞的方案
Supreet Khanal,Nirjal Bhattarai
Supreet Khanal
Chimeric antigen receptor-expressing NK (CAR-NK) cells represent an advancing frontier in cancer immunotherapy, building upon decades of natural killer cell research and recent breakthroughs in CAR technology. While early CAR-NK manufacturi...