Protocol Article: A Cross-Sectional Evaluation of Children's Feet and Lower Extremities [0.03%]
协议文章：儿童的足部和下肢横断面评估
Christian Wong,Christina Ystrøm Bjerge,Ales Jurca et al.
Christian Wong et al.
Background: The health of children's lower extremities and feet is a focus area for caregivers and healthcare professionals such as doctors, school nurses, and podiatrists. Our study aims to investigate the general health...
Validity and Reliability of POM-Checker for Measuring Shoulder Range of Motion in Healthy Participants: A Pilot Single-Center Comparative Study [0.03%]
POM-Checker评估健康受试者肩关节活动范围的有效性和可靠性：一项单中心的先导比较研究
Hongmin Chu,Weonjin Kim,Seongsu Joo et al.
Hongmin Chu et al.
Background: The aim of this study was to compare shoulder movement measurements between a Kinect-based markerless ROM assessment device (POM-Checker) and a 3D motion capture analysis system (BTS SMART DX-400). ...
A Versatile Protocol for Efficient Transformation and Regeneration in Mega Indica Rice Cultivar MTU1010: Optimization through Hormonal Variables [0.03%]
适用于巨型籼稻品种MTU1010高效转化与再生的通用协议：通过激素变量进行优化
Pragya Yadav,V V Santosh Kumar,Jyoti Priya et al.
Pragya Yadav et al.
Rice is one of the apex food crops in terms of meeting the daily calorific and dietary requirement of the majority of the world population. However, rice productivity is severely limited by various biotic and abiotic attributes, causing a s...
A Simple, Rapid, and Effective Heparinase Protocol to Enable Nucleic Acid Study from Frozen Heparinized Plasma [0.03%]
一种简单的用于从冻存肝素化血浆中获取核酸的他扎林酶处理方法
Rownock Afruza,Nicole Minerva,Justin B Lack et al.
Rownock Afruza et al.
Cell-free RNAs (cfRNAs) are promising analytes as non-invasive biomarkers and have even greater potential if tied in with metabolomics. Plasma is an optimal source for cfRNAs but is often derived from a variety of anticoagulants. Plasma obt...
Fernando Albericio,Philip Hublitz
Fernando Albericio
As our readers know, Methods and Protocols is a multidisciplinary peer-reviewed scientific journal that provides a forum to the publication of novel approaches in the fields of Life Sciences, Chemistry, and Biomedical Sciences and their int...
Protocol for Facile Synthesis of Fmoc-N-Me-AA-OH Using 2-CTC Resin as Temporary and Reusable Protecting Group [0.03%]
基于2-CTC树脂临时可回收保护的Fmoc-N-Me-AA-OH类衍砌筑化合物的简便合成方法学研究协议
Tanya Román,Gerardo Acosta,Constanza Cárdenas et al.
Tanya Román et al.
One approach to enhance the bioavailability and half-life of peptides in vivo is through N-methylation of one or more of the amino acids within the peptide sequence. However, commercially available Fmoc-N-Me-AA-OHs are limited and often exp...
Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples [0.03%]
生物分子互作分析的指数富集配体系统进化方法及其在筛选疾病标志物中的应用研究进展
Juan David Ospina-Villa,Valentina Restrepo-Cano,Miryan Margot Sánchez-Jiménez
Juan David Ospina-Villa
Bio-SELEX is a revolutionary method for the discovery of novel biomarkers within biological samples, offering profound insights into diagnosing both infectious and non-infectious diseases. This innovative strategy involves three crucial ste...
Comparison of Light-Sheet Fluorescence Microscopy and Fast-Confocal Microscopy for Three-Dimensional Imaging of Cleared Mouse Brain [0.03%]
清醒小鼠脑三维成像中的轻片荧光显微镜与快速共聚焦显微镜的比较研究
Youngjae Ryu,Yoonju Kim,Sang-Joon Park et al.
Youngjae Ryu et al.
Whole-brain imaging is important for understanding brain functions through deciphering tissue structures, neuronal circuits, and single-neuron tracing. Thus, many clearing methods have been developed to acquire whole-brain images or images ...
Development of a Quantitative PCR Method for Detecting Enterococcus faecalis Cytolysin in Human Stool Samples [0.03%]
人粪便样本中肠球菌胞裂素检测的定量PCR方法开发
Noemí Cabré,Yongqiang Yang,Yanhan Wang et al.
Noemí Cabré et al.
Alcohol-associated liver disease (ALD) is a major global health issue, contributing significantly to morbidity and mortality worldwide. Among the ALD subtypes, alcohol-associated hepatitis poses a severe and urgent medical challenge with hi...
Improved Separation in Horizontal Protein SDS-PAGE with Double-Deck Flat Electrodes and a Field Inversion Gel Electrophoresis Module [0.03%]
改进的水平蛋白质SDS-PAGE在双层平板电极和场反向凝胶电泳模块中的分离性能
Dong Woo Lim,Tae-Sung Yoon,Kyung Ho Han et al.
Dong Woo Lim et al.
The horizontal flatbed electrophoresis method is employed to separate protein samples, providing greater flexibility for various electrophoretic applications and easier sample loading compared to its vertical counterpart. In the currently a...