Exploration of endogenous signal peptides and tandem linkage strategies for enhanced heterologous protein secretion in Lacticaseibacillus paracasei [0.03%]
探索内源信号肽及串联链接策略以增强副干酪乳杆菌中异源蛋白分泌性能
Xiaojing Li,Siyuan Zhu,Xiting Huang et al.
Xiaojing Li et al.
Lacticaseibacillus paracasei (L. paracasei), a probiotic bacterium commonly found in the intestinal tract and fermented products, has been utilized as a tool for generating various bioactive components. However, its potential as a host cell...
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production [0.03%]
代谢与进化工程集成构建用于纤维素乙醇生产的高效木糖利用菌株ZmobilisCX5
Jiyun Lou,Xia Wang,Runxia Li et al.
Jiyun Lou et al.
Xylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production. Zymomonas mobilis is a natural ethanologenic bac...
Enhanced AAV production via rational design of a novel pHelper vector integrated with HSV-1 helper genes [0.03%]
一种新型含单纯疱疹病毒辅助基因的pHelper载体的理性设计及对AAV生产的增强作用研究
He Ren,Jianqi Nie,Zichuan Song et al.
He Ren et al.
Adeno-associated virus (AAV) vectors are widely used in gene therapy owing to their safety, stability, and broad tissue tropism. However, current plasmid-based AAV manufacturing platforms suffer from low yield and high manufacturing cost, l...
The functional analysis of NirBD in microorganisms and the efficacies of NirBD on the soil nitrogen storage and N2O emission [0.03%]
NirBD的功能分析及其对土壤氮素存储和氧化亚氮排放的影响研究
Yidan Peng,Tengxia He,Qimin Zhou et al.
Yidan Peng et al.
The siroheme-containing nitrite reductase (NirBD), which is encoded by the nirBD gene, is a functional enzyme in the nitrogen cycle. The NirBD enzyme can regulate N2O release through the two distinct pathways of assimilatory nitrate/nitrite...
De novo synthesis of L-2-aminobutyric acid in Escherichia coli based on multi-layered metabolic engineering strategies [0.03%]
基于多层代谢工程技术从头合成L-2-氨基丁酸
Zhenqiang Zhao,Yizheng Liu,Rongshuai Zhu et al.
Zhenqiang Zhao et al.
L-2-Aminobutyric acid (L-2-ABA) is a non-proteinogenic amino acid and an important chiral intermediate widely used in pharmaceuticals and fine chemicals. However, its fermentative production is limited by intermediate toxicity and imbalance...
Integrated amplification of NADPH-regenerating modules enhances cytidine biosynthesis in Escherichia coli [0.03%]
集成放大NADPH再生模块可增强大肠杆菌中胞苷生物合成
Lu Liu,Xiangjun Zhang,Tengteng Zhu et al.
Lu Liu et al.
In Escherichia coli, cofactor imbalance serves as a crucial limiting factor in cytidine biosynthesis, with nicotinamide adenine dinucleotide phosphate (NADPH) insufficiency representing the principal metabolic barrier. To overcome this limi...
RECC: A Red/ET-CRISPR/Cas9-based system enabling genome mining of marine Pseudoalteromonas for novel natural products [0.03%]
RECC系统：基于红/ET-Cas9技术的海洋假单胞菌基因组挖掘体系用于新型次级代谢产物发现研究
Zong-Jie Wang,Haibo Zhou,Youming Zhang et al.
Zong-Jie Wang et al.
Marine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions. Genetic intractability has been a major barrier to activating these ...
Multi-gene Co-expression systems in E. coli: From single-vector designs to programmable expression platforms [0.03%]
大肠杆菌多基因共表达系统：从单质粒设计到可编程表达平台
Rui Liu,Lu-Wei Wang,Zi-Han Gao et al.
Rui Liu et al.
Escherichia coli (E. coli) has long served as a versatile workhorse for recombinant protein production. As synthetic biology expands the demand for coordinated expression of multiple genes, co-expression systems in E. coli have evolved from...
Metabolic reprogramming of abscisic acid-producing strain Botrytis cinerea TB-31 toward terpenoid biosynthesis using a CRISPR/Cas9 ribonucleoprotein system [0.03%]
利用CRISPR / Cas9核糖核蛋白系统对产脱落酸菌Botrytis cinerea TB-31进行代谢重构以促进萜类生物合成
Xiao-Nan Hou,Dan Shu,Tian-Fu Li et al.
Xiao-Nan Hou et al.
Compared with conventional microbial hosts, filamentous fungi have distinct advantages for the industrial-scale biosynthesis of high-value chemical compounds. However, current research on strain engineering and fermentation optimization str...
Yunyue Chen,Siyifei Wang,Leiying Xie et al.
Yunyue Chen et al.
Precise regulation of protein abundance is essential for understanding dynamic cellular processes and for advancing therapeutic development. However, existing approaches lack the spatiotemporal resolution required to these cellular processe...