Improved production of cytotoxic thailanstatins A and D through metabolic engineering of Burkholderia thailandensis MSMB43 and pilot scale fermentation [0.03%]
通过Burkholderia thailandensis MSMB43代谢工程改进细胞毒thailanstatins A和D的生产并进行中试发酵
Xiangyang Liu,Hui Zhu,Sreya Biswas et al.
Xiangyang Liu et al.
Thailanstatin A (TST-A) is a potent antiproliferative natural product discovered by our group from Burkholderia thailandensis MSMB43 through a genome-guided approach. The limited supply of TST-A, due to its low titer in bacterial fermentati...
In vitro reconstitution guide for targeted synthetic metabolism of chemicals, nutraceuticals and drug precursors [0.03%]
化学、营养药品和药物前体靶向合成代谢的体外重组指南
Gao-Yi Tan,Faying Zhu,Zixin Deng et al.
Gao-Yi Tan et al.
With the developments in metabolic engineering and the emergence of synthetic biology, many breakthroughs in medicinal, biological and chemical products as well as biofuels have been achieved in recent decades. As an important barrier to tr...
J Satiaputra,K E Shearwin,G W Booker et al.
J Satiaputra et al.
Biotin is an essential micronutrient that acts as a co-factor for biotin-dependent metabolic enzymes. In bacteria, the supply of biotin can be achieved by de novo synthesis or import from exogenous sources. Certain bacteria are able to obta...
Interrogation of Streptomyces avermitilis for efficient production of avermectins [0.03%]
灰霉素亚胺霉菌 avermectin 的高效产生及产量基因的挖掘与改造
Jinsong Chen,Mei Liu,Xueting Liu et al.
Jinsong Chen et al.
The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin, respectively. Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents. Because wild-type str...
Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis [0.03%]
酵母敲除库可有效用于无细胞蛋白质合成的基因组突变检测
Jennifer A Schoborg,Lauren G Clark,Alaksh Choudhury et al.
Jennifer A Schoborg et al.
Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein s...
Lixin Zhang
Lixin Zhang
Enhancing rufomycin production by CRISPR/Cas9-based genome editing and promoter engineering in Streptomyces sp. MJM3502 [0.03%]
利用CRISPR/Cas9基因组编辑和启动子工程提高链霉菌MJM3502产rufomycin水平
Streptomyces sp. MJM3502 is a promising producer of rufomycins, which are a class of potent anti-tuberculosis lead compounds. Although the structure, activity, and mechanism of the main rufomycin 4/6 and its analogs have been extensively st...