Metabolic engineering of endogenous MEP pathway for enhanced lycopene production in Escherichia coli [0.03%]
利用内源MEP途径代谢工程增强大肠杆菌中番茄红素的生产
Xian Xu,Hongyu Xing,Hui Zhi et al.
Xian Xu et al.
Microbial cell factories represent the primary approach for heterologous lycopene synthesis, where gene source selection and pathway regulation have been demonstrated to have a significant impact on lycopene titer. In this study, key lycope...
Targeted identification of new phaterpenes and elucidation of the relevant biosynthetic pathway in Streptomyces phaeochromogenes OSK-123 [0.03%]
链霉菌OSK-123中新的放线菌素的靶向鉴定及其生物合成途径解析
Xing Fan,Xin Zhang,Minguo Tang et al.
Xing Fan et al.
Sesquiterpenes have highly diverse chemical structures and biological activities, resulting in considerable interest in terms of their application and exploration of new analogs. In this study, we identified a new cryptic bacterial terpenoi...
Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects [0.03%]
微生物细胞工厂中戊二酸的可持续生产：当前进展和未来前景
Jie Liu,Dan Mei,Xuan-Jun Zhang et al.
Jie Liu et al.
Glutaric acid is a significant C5 dicarboxylic acid, extensively utilized in the chemical industry, medicine, and biomaterials. In recent years, the advancement of synthetic biology and metabolic engineering has rendered microbial productio...
A novel LPS-dependent outer membrane-anchoring mechanism for T9SS substrates enables engineered enzyme display and whole-cell PET degradation in Cytophaga hutchinsonii [0.03%]
霍乱弧菌新型LPS依赖的外膜锚定机制使芽孢杆菌中工程酶展示和全细胞PET降解成为可能
Wenxia Song,Sheng Yang,Rong Tan et al.
Wenxia Song et al.
Robust surface display systems are crucial for engineering Gram-negative bacteria as whole-cell biocatalysts. In the efficient cellulose degrader Cytophaga hutchinsonii, cellulases are secreted by the Type IX Secretion System (T9SS), yet th...
Xinyu Liu,Qihang Chen,Wenbao Zhao et al.
Xinyu Liu et al.
Ergosterol is the key precursor of steroid drug synthesis. In this experiment, we systematically modified the synthesis of ergosterol. Firstly, we identified key rate-limiting enzymes through systematic screening of the post-squalene pathwa...
A synergistic strategy of metabolic engineering and flocculation recycling for enhanced acetoin production in Zymomonas mobilis [0.03%]
协同代谢工程和絮凝回收的合成策略用于提高Zymomonas mobilis生产乙偶姻效价
Weiwei Bao,Qiqun Peng,Hongxiang Yu et al.
Weiwei Bao et al.
Acetoin, a valuable platform chemical, faces sustainability challenges in its traditional energy-intensive synthesis. Microbial fermentation using microorganisms such as Zymomonas mobilis offers a promising alternative. To overcome metaboli...
Modifying the upstream open reading frames of cellulase gene enhances cellulase production in Penicillium oxalicum [0.03%]
拟青霉细胞壁降解酶基因上游开放阅读框的改造及对纤维素酶表达的影响研究
Shuai Zhao,Xin Shen,Ting-Yu Zhou et al.
Shuai Zhao et al.
Cellulase plays an irreplaceable role in biomanufacturing using plant biomass as feedstock. However, improving cellulase production by fungi through manipulation of upstream open reading frames (uORFs) in the 5'-untranslated regions (5'-UTR...
Engineering Escherichia coli for robust Co-utilization of glucose and xylose enables high-titer succinate production from lignocellulosic hydrolysates [0.03%]
工程改造大肠杆菌高效利用葡萄糖和木糖生产琥珀酸用于木质纤维素水解液中高浓度生产琥珀酸
Jiugong Lv,Jiuquan An,Zhenyu Sun et al.
Jiugong Lv et al.
Succinate is a biobased platform chemical with wide applications in food, pharmaceuticals, and biodegradable polymers such as polybutylene succinate. Despite advances in microbial fermentation, cost-effective production remains limited by i...
Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology [0.03%]
仿生无膜细胞器的工程化进展及其生物技术应用
Manman Sun,Alex Xiong Gao,Bin Ye et al.
Manman Sun et al.
Membraneless organelles (MLOs) formed through liquid-liquid phase separation (LLPS) constitute crucial dynamic microenvironments within cells, capable of selectively concentrating specific molecules and regulating biochemical reactions. Bas...
Engineering Kluyveromyces marxianus for 3-hydroxypropionic acid production at elevated temperature from Jerusalem artichoke tubers and crude glycerol [0.03%]
高温菊芋块茎和粗甘油生物合成3-羟基丙酸工程酵母菌KLUYVEROMYCES MARXIANUS的研究
Jiacheng Li,Zhongmei Hu,Yanjie Li et al.
Jiacheng Li et al.
This study engineered the thermotolerant yeast Kluyveromyces marxianus to produce 3-hydroxypropionic acid (3-HP), a key precursor for biodegradable plastics, via the malonyl-CoA pathway using non-food feedstocks. The 3-HP titer was further ...