Cell lysates and egg white create homeostatic microenvironment for gene expression in cell-free system [0.03%]
细胞裂解产物和蛋清在无细胞系统中构建基因表达的生理环境
Yang Liu,Dayong Yang
Yang Liu
Homeostasis widely exists in living systems, and plays essential roles for maintaining normal physiological activities, enabling to preserve their functionalities against variations. Gene expression is a crucial process that allows cells to...
Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions [0.03%]
通过最小化反应的细胞 free 生物系统将d-葡萄糖转化为乳酸的最佳方案
Leipeng Xie,Xinlei Wei,Xigui Zhou et al.
Leipeng Xie et al.
Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired product...
A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue [0.03%]
一种能感应并降解四环素抗生素残留的遗传工程菌株大肠杆菌菌株
Zepeng Mu,Zhuoning Zou,Ye Yang et al.
Zepeng Mu et al.
Due to the abuse of antibiotics, antibiotic residues can be detected in both natural environment and various industrial products, posing threat to the environment and human health. Here we describe the design and implementation of an engine...
An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design [0.03%]
一种体外合成生物学平台：自下而上的途径设计用于新兴工业生物制造
Ting Shi,Pingping Han,Chun You et al.
Ting Shi et al.
Although most in vitro (cell-free) synthetic biology projects are usually used for the purposes of fundamental research or the formation of high-value products, in vitro synthetic biology platform, which can implement complicated biochemica...
Wen Si,Cheng Li,Ping Wei
Wen Si
During the past decades, the rapidly-evolving cancer is hard to be thoroughly eliminated even though the radiotherapy and chemotherapy do exhibit efficacy in some degree. However, a breakthrough appeared when the adoptive cancer therapy [1]...
Activation of microbial secondary metabolic pathways: Avenues and challenges [0.03%]
微生物次级代谢途径的激活：通路与挑战
Bikash Baral,Amir Akhgari,Mikko Metsä-Ketelä
Bikash Baral
Microbial natural products are a tremendous source of new bioactive chemical entities for drug discovery. Next generation sequencing has revealed an unprecedented genomic potential for production of secondary metabolites by diverse micro-or...
Wei Ding,Tianlu Mo,Dhanaraju Mandalapu et al.
Wei Ding et al.
The linaridin antibiotic cypemycin is a ribosomal synthesized and post-translationally modified peptide (RiPP) that possesses potent activity against mouse leukemia cells. This peptide natural product contains an S-[(Z)-2-aminovinyl]-d-cyst...
Yujia Huang,Tao Liu
Yujia Huang
In nature, a limited, conservative set of amino acids are utilized to synthesize proteins. Genetic code expansion technique reassigns codons and incorporates noncanonical amino acids (ncAAs) through orthogonal aminoacyl-tRNA synthetase (aaR...
CRISPR-Cas9/Cas12a biotechnology and application in bacteria [0.03%]
基于CRISPR-Cas9/Cas12a的细菌基因编辑技术及应用进展
Ruilian Yao,Di Liu,Xiao Jia et al.
Ruilian Yao et al.
CRISPR-Cas technologies have greatly reshaped the biology field. In this review, we discuss the CRISPR-Cas with a particular focus on the associated technologies and applications of CRISPR-Cas9 and CRISPR-Cas12a, which have been most widely...
Puromycin A, B and C, cryptic nucleosides identified from Streptomyces alboniger NRRL B-1832 by PPtase-based activation [0.03%]
由磷蛋白酶激活的链霉菌NRRL B-1832中的隐匿核苷puromycin A、B和C
Xiaoli Yan,Benyin Zhang,Wenya Tian et al.
Xiaoli Yan et al.
Natural product discovery is pivot for drug development, however, this endeavor is often challenged by the wide inactivation or silence of natural products biosynthetic pathways. We recently developed a highly efficient approach to activate...