Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis [0.03%]
解码盐单胞菌生长与四氢基异噻唑生产之间的代谢权衡：基于流速重构的连续培养法生产工业四氢基异噻唑
Junxiong Yu,Hao Liu,Yudi Wang et al.
Junxiong Yu et al.
Halomonas elongata, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine-a high-value compatible solute extensively utilized in biopharmaceuticals and cosmetics. While its ectoine...
Enzyme and cofactor engineering to increase d-xylonate dehydratase activity for improved d-1,2,4-butanetriol production from d-xylose [0.03%]
酶和辅因子工程增强D-木糖酸脱水酶活性以提高D-阿拉伯糖醇的生产率
Jingwen Chen,Zhangyu Liu,Dandan Mai et al.
Jingwen Chen et al.
d-1,2,4-Butanetriol (BTO), a C4 platform compound, is widely used in fields such as military and pharmaceuticals. Biosynthesis of d-1,2,4-BTO from lignocellulose-derived d-xylose presents a promising production route. However, the low catal...
Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB [0.03%]
通过微型DNA内切酶IscB在枯草芽孢杆菌中实现高效的基因组编辑
Jie Gao,Hengyi Wang,Jingtao Sun et al.
Jie Gao et al.
Existing CRISPR-based genome editing techniques for Bacillus subtilis (B. subtilis) are limited due to the large size of the cas gene. IscB, a recently reported DNA nuclease, is one-third the size of Cas9, making it a potential tool for gen...
Development of precise genome editing and multi-copy integration tools in Hansenula polymorpha DL-1 [0.03%]
Hansenula polymorpha DL-1精确基因组编辑和多拷贝整合工具的开发
Xiaoyi Zou,Jiaqi Miao,Hongbiao Li et al.
Xiaoyi Zou et al.
Hansenula polymorpha DL-1 is a thermotolerant yeast capable of utilizing multiple renewable carbon sources, making it a promising microbial cell factory for sustainable manufacturing. However, advanced metabolic engineering efforts have bee...
A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification [0.03%]
一种多功能开关，用于无标签CRISPR / Cas12a传感器的自驱动放大功能
Po Li,Xueying Lei,Xiaoying Niu et al.
Po Li et al.
MicroRNA (miRNA) is promising candidate for non-invasive diagnostic biomarker. Conventional CRISPR/Cas12a-based miRNA detection systems are constrained by reliance on reverse transcription, nucleic acid pre-amplification and costly fluoresc...
Extracellular vesicles enhance the efficacy of ceftiofur against intracellular bacterial infections [0.03%]
细胞外囊泡可增强头孢曲松对抗胞内细菌感染的疗效
Pingping Yuan,Shijie Zhou,Qianqian Li et al.
Pingping Yuan et al.
Bacterial infections pose a major threat to human health and economic stability. In particular, intracellular bacterial infections present significant clinical challenges due to antibiotic resistance and poor drug penetration. Therefore, th...
Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies [0.03%]
基于组合代谢策略改造毕赤酵母高效生产β-芹芳烃
Mingyu Dai,Qingyi Cao,Shuanglong Jia et al.
Mingyu Dai et al.
β-Elemene, a pharmacologically active sesquiterpene derived from the traditional Chinese medicinal herb Curcuma wenyujin, exhibits broad-spectrum anti-tumor and anti-inflammatory activities. Currently, its commercial production relies heav...
Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium stationis and its application in hypoxanthine biosynthesis [0.03%]
用于链霉色杆菌(Corynebacterium stationary)的CRISPR / Cas9基因组编辑工具箱的开发及其在次黄嘌呤生物合成中的应用
Zhilin Ouyang,Xinyu Zhang,Xinyi Hou et al.
Zhilin Ouyang et al.
Corynebacterium stationis, a high-GC Gram-positive bacterium with significant industrial potential, has faced limitations due to the lack of efficient genetic tools. In this study, we developed a CRISPR/Cas9-based genome editing platform sp...
From consultors to collaborators - An SOP for advancing ethics engagement in science [0.03%]
从咨询者到合作者——促进科学伦理参与的SOP
Varsha Aravind Paleri,Kristien Hens
Varsha Aravind Paleri
Synthetic Biology (SynBio) is an interdisciplinary field that tackles global challenges in healthcare, sustainability, food production, among others. However, it also presents critical ethical concerns, including biosecurity, justice, and t...
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis [0.03%]
工程化T7RNA聚合酶级联系统，由nisin和茶碱控制，用于乳酸菌中蛋白质过表达及目标基因突变
Ying Huang,Kang Ma,Yan Li et al.
Ying Huang et al.
Lactococcus lactis serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression. However, the NICE system relies on t...