Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue [0.03%]
工程菌乙醇梭状芽孢杆菌Zymomonas mobilis利用农业废弃物玉米芯生产经济型纤维素燃料乙醇
Yongfu Yang,Chenyue Zhang,Binan Geng et al.
Yongfu Yang et al.
Cellulosic ethanol is an important biofuel derived from lignocellulosic biomass, which has the potential to mitigate environmental pollution and contribute to achieving carbon neutralization for a sustainable circular economy. However, the ...
Engineering of multiple modules to enhance lignocellulose degradation ability in Bacillus subtilis using CRISPR/Cas9 system [0.03%]
使用CRISPR / Cas9系统在芽孢杆菌中工程化多个模块以增强降解木质纤维素的能力
Zhiwei Wang,Gongwei Liu,Zhongming Meng et al.
Zhiwei Wang et al.
Bacillus subtilis is widely employed for lignocellulose degradation. However, wild-type strains typically exhibit low and incomplete cellulase activities. This study aimed to engineer recombinant B. subtilis strains harboring complete, high...
High-throughput editing boosts the construction of next-generation microbial cell factories [0.03%]
高通量细胞编辑技术助力下一代微生物细胞工厂构建
Xi Sun,Yangyang Zheng,Jiancheng Luo et al.
Xi Sun et al.
Traditional metabolic engineering faces numerous challenges in constructing microbial cell factories, such as inefficient gene editing, time-consuming and labor-intensive screening processes, and the complexity of multi-gene optimization. H...
Multistep metabolic engineered Klebsiella oxytoca for efficient l-leucine production [0.03%]
高效的代谢工程 Klebsiella oxytoca 生产L-亮氨酸
Weikang Sun,Qiaoyue Yang,Shuo Wang et al.
Weikang Sun et al.
l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain Klebsiella oxytoca LKO-14 was constructed based on an l-valine producer K. oxytoca VKO-9. The exogenous l-leucine biosyn...
De novo biosynthesis of eriocitrin in Saccharomyces cerevisiae through deep learning-guided enzyme screening and systematic metabolic engineering [0.03%]
基于从头合成策略利用酿酒酵母生产橙皮素-7-葡萄糖苷的代谢工程研究
Qiyuan Lu,Xinjia Tan,Siqi Zhang et al.
Qiyuan Lu et al.
Eriocitrin, a flavanone-7-O-disaccharide known for its antioxidant and anti-inflammatory properties, holds considerable promise for use in functional foods and pharmaceuticals. However, its large-scale production is constrained by the limit...
Construction of a globally modular recombinant Saccharomyces cerevisiae chassis for ginkgolide precursor levopimaradiene production [0.03%]
用于银杏萜内酯前体左松三烯生产的全局模块化重组酿酒酵母底盘的构建
De Liu,Ziqing Chen,Zizheng Guo et al.
De Liu et al.
The production of biologically active natural products through microbial metabolic factories, such as Saccharomyces cerevisiae, represents a promising approach to achieving green and sustainable production. However, establishing a stable me...
A novel TetR-type repressor directly modulates precursor supply and utilization for erythromycin biosynthesis [0.03%]
一种新型TetR型阻遏蛋白通过直接调控红霉素生物合成前体的供应与利用参与红霉素合成调节
Panpan Wu,Zhongqiu Meng,Yuling Shu et al.
Panpan Wu et al.
Antibiotic biosynthesis in actinomycetes is controlled by various transcription factors, with TetR family regulators (TFRs) serving as important modulators of this process. We previously discovered a TFR, SACE_1906, which hinders erythromyc...
Reconstructing the hydrogenobyrinic acid synthetic toolkit by combining cell-free systems and metabolic engineering [0.03%]
结合无细胞体系和代谢工程重构氢化藻胆素生物合成途径
Rongyu Lv,Qian Kang,Jiayu Wang et al.
Rongyu Lv et al.
Hydrogenobyrinic acid (HBA) is a pivotal intermediate in the aerobic biosynthesis of cobalamin (vitamin B12), representing a stable, metal-free corrin precursor and a useful substrate for both biological and chemical derivatization. Despite...
Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica [0.03%]
代谢工程和适应性实验室进化提高假丝酵母中的法尼烯生产效率
Qiao-Qin Zhao,Peng-Cheng Hu,Chuan-Jiang Zhang et al.
Qiao-Qin Zhao et al.
Squalene, a multifunctional natural compound with diverse bioactivities, has significant potential in the nutraceutical and health industries. Microbial synthesis using engineered cell factories represents a sustainable alternative to conve...
High-level prodigiosin production in Pseudomonas putida enabled by combinatorial metabolic engineering [0.03%]
系统代谢工程高效生产红色抗生素普罗迪吉红色素种族菌株浦黄菌亚种露斯比氏亚种
Yuxin Zhang,Meiyan Wang,Kaijie Dou et al.
Yuxin Zhang et al.
Prodigiosin, a bioactive tripyrrole pigment, exhibits a broad spectrum of biological activities-including antimicrobial, anticancer, and antimalarial properties-thereby holding significant promise for use in pharmaceutical applications and ...