J Satiaputra,K E Shearwin,G W Booker et al.
J Satiaputra et al.
Biotin is an essential micronutrient that acts as a co-factor for biotin-dependent metabolic enzymes. In bacteria, the supply of biotin can be achieved by de novo synthesis or import from exogenous sources. Certain bacteria are able to obta...
Interrogation of Streptomyces avermitilis for efficient production of avermectins [0.03%]
灰霉素亚胺霉菌 avermectin 的高效产生及产量基因的挖掘与改造
Jinsong Chen,Mei Liu,Xueting Liu et al.
Jinsong Chen et al.
The 2015 Nobel Prize in Physiology or Medicine has been awarded to avermectins and artemisinin, respectively. Avermectins produced by Streptomyces avermitilis are excellent anthelmintic and potential antibiotic agents. Because wild-type str...
Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis [0.03%]
酵母敲除库可有效用于无细胞蛋白质合成的基因组突变检测
Jennifer A Schoborg,Lauren G Clark,Alaksh Choudhury et al.
Jennifer A Schoborg et al.
Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein s...
Lixin Zhang
Lixin Zhang
Enhancing rufomycin production by CRISPR/Cas9-based genome editing and promoter engineering in Streptomyces sp. MJM3502 [0.03%]
利用CRISPR/Cas9基因组编辑和启动子工程提高链霉菌MJM3502产rufomycin水平
Streptomyces sp. MJM3502 is a promising producer of rufomycins, which are a class of potent anti-tuberculosis lead compounds. Although the structure, activity, and mechanism of the main rufomycin 4/6 and its analogs have been extensively st...