Sequence homolog-based molecular engineering for shifting the enzymatic pH optimum [0.03%]
基于序列同源性的分子工程学在改变酶的最适PH值中的应用
Fuqiang Ma,Yuan Xie,Manjie Luo et al.
Fuqiang Ma et al.
Cell-free synthetic biology system organizes multiple enzymes (parts) from different sources to implement unnatural catalytic functions. Highly adaption between the catalytic parts is crucial for building up efficient artificial biosyntheti...
Discovery of tanshinone derivatives with anti-MRSA activity via targeted bio-transformation [0.03%]
通过靶向生物转化发现具有抗MRSA活性的丹参新醌类成分
Wenni He,Miaomiao Liu,Pei Huang et al.
Wenni He et al.
Two potent anti-MRSA tanshinone glycosides (1 and 2) were discovered by targeted microbial biotransformation, along with rapid identification via MS/MS networking. Serial reactions including dehydrogenation, demethylations, reduction, glyco...
A systematic study of the whole genome sequence of Amycolatopsis methanolica strain 239T provides an insight into its physiological and taxonomic properties which correlate with its position in the genus [0.03%]
对棒状放线菌全基因组序列的系统研究揭示了与其属内分类学地位相关的生理和分类学特性
Biao Tang,Feng Xie,Wei Zhao et al.
Biao Tang et al.
The complete genome of methanol-utilizing Amycolatopsis methanolica strain 239T was generated, revealing a single 7,237,391 nucleotide circular chromosome with 7074 annotated protein-coding sequences (CDSs). Comparative analyses against the...
Beauvericin counteracted multi-drug resistant Candida albicans by blocking ABC transporters [0.03%]
Beauvericin通过阻断ABC转运蛋白对抗多重耐药的白色念珠菌
Yaojun Tong,Mei Liu,Yu Zhang et al.
Yaojun Tong et al.
Multi-drug resistance of pathogenic microorganisms is becoming a serious threat, particularly to immunocompromised populations. The high mortality of systematic fungal infections necessitates novel antifungal drugs and therapies. Unfortunat...
Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification [0.03%]
采用蛋白质标准绝对定量法对脂肪酸生物合成途径中的蛋白进行绝对定量
Hui Tao,Yuchen Zhang,Xiaoying Cao et al.
Hui Tao et al.
With worldwide attention on renewable energy and climate change, metabolic engineering of the fatty acid biosynthetic pathway has become an active area of research, with a view to enhance production of biofuels. Indeed, this pathway has alr...
Study on genetic engineering of Acremonium chrysogenum, the cephalosporin C producer [0.03%]
产头孢菌素C生物菌株链丝霉黄枝青霉的分子改造研究
Youjia Hu,Baoquan Zhu
Youjia Hu
Acremonium chrysogenum is an important filamentous fungus which produces cephalosporin C in industry. This review summarized the study on genetic engineering of Acremonium chrysogenum, including biosynthesis and regulation for fermentation ...
The application of microfluidic-based technologies in the cycle of metabolic engineering [0.03%]
基于微流体技术在代谢工程循环中的应用
Xiaoyan Ma,Yi-Xin Huo
Xiaoyan Ma
The process of metabolic engineering consists of multiple cycles of design, build, test and learn, which is typically laborious and time-consuming. To increase the efficiency and the rate of success of strain engineering, novel instrumentat...
Informatic analysis reveals Legionella as a source of novel natural products [0.03%]
信息分析发现 legionalla 是新型天然产物的来源
Chad W Johnston,Jonathan Plumb,Xiang Li et al.
Chad W Johnston et al.
Microbial natural products are a crucial source of bioactive molecules and unique chemical scaffolds. Despite their importance, rediscovery of known natural products from established productive microbes has led to declining interest, even w...
FunGeneClusterS: Predicting fungal gene clusters from genome and transcriptome data [0.03%]
FunGeneClusterS：从基因组和转录组数据中预测真菌基因簇
Tammi C Vesth,Julian Brandl,Mikael Rørdam Andersen
Tammi C Vesth
Introduction: Secondary metabolites of fungi are receiving an increasing amount of interest due to their prolific bioactivities and the fact that fungal biosynthesis of secondary metabolites often occurs from co-regulated...
CRISPy-web: An online resource to design sgRNAs for CRISPR applications [0.03%]
CRISPy-web：在线设计CRISPR应用sgRNA资源网站
Kai Blin,Lasse Ebdrup Pedersen,Tilmann Weber et al.
Kai Blin et al.
CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes as an adaptive immune system against ba...