Engineered polyketides: Synergy between protein and host level engineering [0.03%]
工程聚酮类化合物：蛋白质和宿主水平工程的协同作用
Jesus F Barajas,Jacquelyn M Blake-Hedges,Constance B Bailey et al.
Jesus F Barajas et al.
Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities. Polyketides e...
Norine: A powerful resource for novel nonribosomal peptide discovery [0.03%]
Norine：用于新型非核糖体肽发现的强大资源
M Pupin,Q Esmaeel,A Flissi et al.
M Pupin et al.
Since its first release in 2008, Norine remains the unique resource completely devoted to nonribosomal peptides (NRPs). They are very attractive microbial secondary metabolites, displaying a remarkable diversity of structure and functions. ...
Characterization of the metallo-dependent amidohydrolases responsible for "auxiliary" leucinyl removal in the biosynthesis of 2,2'-bipyridine antibiotics [0.03%]
表征金属依赖性酰胺酶在2,2'-联吡啶抗生素生物合成中负责“辅助”亮氨酰移除的特性
Ming Chen,Bo Pang,Ya-Nan Du et al.
Ming Chen et al.
2,2'-Bipyridine (2,2'-BiPy) is an attractive core structure present in a number of biologically active natural products, including the structurally related antibiotics caerulomycins (CAEs) and collismycins (COLs). Their biosynthetic pathway...
Improvement of pristinamycin I (PI) production in Streptomyces pristinaespiralis by metabolic engineering approaches [0.03%]
代谢工程提高链霉菌产生杆菌肽AI产量
Jiali Meng,Rongrong Feng,Guosong Zheng et al.
Jiali Meng et al.
Pristinamycin, produced by Streptomyces pristinaespiralis, which is a streptogramin-like antibiotic consisting of two chemically unrelated components: pristinamycin I (PI) and pristinamycin II (PII), shows potent activity against many antib...
Rational synthetic combination genetic devices boosting high temperature ethanol fermentation [0.03%]
理性的合成组合型基因装置可大幅提升高温乙醇发酵性能
Huan Sun,Haiyang Jia,Jun Li et al.
Huan Sun et al.
The growth and production of yeast in the industrial fermentation are seriously restrained by heat stress and exacerbated by heat induced oxidative stress. In this study, a novel synthetic biology approach was developed to globally boost th...
Systematic assessment of Pichia pastoris system for optimized β -galactosidase production [0.03%]
系统评价毕赤酵母系统优化β-半乳糖苷酶生产
Hongbing Sun,Olufemi Emmanuel Bankefa,Ijeoma Onyinyechi Ijeoma et al.
Hongbing Sun et al.
Streptophage-mediated control of off-flavour taint producing streptomycetes isolated from barramundi ponds [0.03%]
芽孢杆菌噬菌体对拜灵鱼塘中产生异味假单胞菌的控制作用研究
Jodi Anne Jonns,Peter Richard Brooks,Paul Exley et al.
Jodi Anne Jonns et al.
Off-flavour taint of aquaculture products is a global issue reducing consumer confidence in the farmed produce as they are taken up via the gills of fish, and deposited in the lipids of the animal. If the fish are not purged, resulting unde...
Bacterial cell-free expression technology to in vitro systems engineering and optimization [0.03%]
细菌无细胞表达技术在体外系统工程与优化中的应用
Filippo Caschera
Filippo Caschera
Cell-free expression system is a technology for the synthesis of proteins in vitro. The system is a platform for several bioengineering projects, e.g. cell-free metabolic engineering, evolutionary design of experiments, and synthetic minima...
Systems metabolic engineering strategies for the production of amino acids [0.03%]
系统代谢工程策略在氨基酸生产中的应用
Qian Ma,Quanwei Zhang,Qingyang Xu et al.
Qian Ma et al.
Systems metabolic engineering is a multidisciplinary area that integrates systems biology, synthetic biology and evolutionary engineering. It is an efficient approach for strain improvement and process optimization, and has been successfull...
Targeted mutagenesis: A sniper-like diversity generator in microbial engineering [0.03%]
靶向诱变：一种微生物工程中的精准多样性生成技术
Xiang Zheng,Xin-Hui Xing,Chong Zhang
Xiang Zheng
Mutations, serving as the raw materials of evolution, have been extensively utilized to increase the chances of engineering molecules or microbes with tailor-made functions. Global and targeted mutagenesis are two main methods of obtaining ...