Xiao Yi
Xiao Yi
Biological functions - studied by molecular, systems and behavioral biology - are referred to as proximate mechanisms. Why and how they have emerged from the course of evolution are referred to as ultimate mechanisms. Despite the conceptual...
A review of computational tools for design and reconstruction of metabolic pathways [0.03%]
代谢途径设计和重建的计算工具评述
Lin Wang,Satyakam Dash,Chiam Yu Ng et al.
Lin Wang et al.
Metabolic pathways reflect an organism's chemical repertoire and hence their elucidation and design have been a primary goal in metabolic engineering. Various computational methods have been developed to design novel metabolic pathways whil...
Heterologous biosynthesis of natural product naringenin by co-culture engineering [0.03%]
异源共培养合成天然产物柚皮素
Vijaydev Ganesan,Zhenghong Li,Xiaonan Wang et al.
Vijaydev Ganesan et al.
Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, E. coli-E. coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultur...
Guanghong Zuo,Bailin Hao
Guanghong Zuo
A monospecific genus contains a single species ever since it was proposed. Though formally more than half of the known prokaryotic genera are monospecific, we pick up those which actually raise taxonomic problems by violating monophyly of t...
Fundamental CRISPR-Cas9 tools and current applications in microbial systems [0.03%]
基于CRISPR-Cas9的微生物系统基因组编辑工具及应用进展
Pingfang Tian,Jia Wang,Xiaolin Shen et al.
Pingfang Tian et al.
Derived from the bacterial adaptive immune system, CRISPR technology has revolutionized conventional genetic engineering methods and unprecedentedly facilitated strain engineering. In this review, we outline the fundamental CRISPR tools tha...
Protein engineering of oxidoreductases utilizing nicotinamide-based coenzymes, with applications in synthetic biology [0.03%]
基于烟酰胺类辅酶的氧化还原酶蛋白质工程及其在合成生物学中的应用
Chun You,Rui Huang,Xinlei Wei et al.
Chun You et al.
Two natural nicotinamide-based coenzymes (NAD and NADP) are indispensably required by the vast majority of oxidoreductases for catabolism and anabolism, respectively. Most NAD(P)-dependent oxidoreductases prefer one coenzyme as an electron ...
Genome and metabolic engineering in non-conventional yeasts: Current advances and applications [0.03%]
非传统酵母的基因组和代谢工程：当前进展与应用
Ann-Kathrin Löbs,Cory Schwartz,Ian Wheeldon
Ann-Kathrin Löbs
Microbial production of chemicals and proteins from biomass-derived and waste sugar streams is a rapidly growing area of research and development. While the model yeast Saccharomyces cerevisiae is an excellent host for the conversion of glu...
Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis [0.03%]
增强细菌多羟基烷酸(PHA)生物合成性能的工程菌株构建及代谢机制研究
Guo-Qiang Chen,Xiao-Ran Jiang
Guo-Qiang Chen
Polyhydroxyalkanoates (PHA) have been produced by some bacteria as bioplastics for many years. Yet their commercialization is still on the way. A few issues are related to the difficulty of PHA commercialization: namely, high cost and insta...
Michael D Engstrom,Brian F Pfleger
Michael D Engstrom
In synthetic biology, researchers assemble biological components in new ways to produce systems with practical applications. One of these practical applications is control of the flow of genetic information (from nucleic acid to protein), a...
Metabolic engineering for the microbial production of isoprenoids: Carotenoids and isoprenoid-based biofuels [0.03%]
代谢工程在微生物异戊二烯化合物生产中的应用：类胡萝卜素和基于异戊二烯的生物燃料
Fu-Xing Niu,Qian Lu,Yi-Fan Bu et al.
Fu-Xing Niu et al.
Isoprenoids are the most abundant and highly diverse group of natural products. Many isoprenoids have been used for pharmaceuticals, nutraceuticals, flavors, cosmetics, food additives and biofuels. Carotenoids and isoprenoid-based biofuels ...