Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology [0.03%]
无细胞蛋白质合成推动代谢工程与合成生物学的快速构建及应用
Lihong Jiang,Jiarun Zhao,Jiazhang Lian et al.
Lihong Jiang et al.
Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular me...
Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products [0.03%]
无细胞合成生物学在体外生物合成药物天然产物中的应用
Jian Li,Lingkai Zhang,Wanqiu Liu
Jian Li
Natural products with significant biological activities continuously act as rich sources for drug discovery and development. To harness the potential of these valuable compounds, robust methods need to be developed for their rapid and susta...
Aman S Imani,Michael F Freeman
Aman S Imani
Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX [0.03%]
利用yTREX快速生成产生荧光假单胞菌次级代谢产物的重组菌株
Andreas Domröse,Robin Weihmann,Stephan Thies et al.
Andreas Domröse et al.
Microbial secondary metabolites represent a rich source of valuable compounds with a variety of applications in medicine or agriculture. Effective exploitation of this wealth of chemicals requires the functional expression of the respective...
Improving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp. SE50/110 [0.03%]
利用放线计划菌SE50/110高效遗传操作体系提高阿卡波糖产量并消除副产物组分C
Qinqin Zhao,Huixin Xie,Yao Peng et al.
Qinqin Zhao et al.
The α-glucosidase inhibitor acarbose is commercially produced by Actinoplanes sp. and used as a potent drug in the treatment of type-2 diabetes. In order to improve the yield of acarbose, an efficient genetic manipulation system for Actino...
Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression [0.03%]
快速进化调节元件库以实现基因表达的可调转录和翻译控制
Erqing Jin,Lynn Wong,Yun Jiao et al.
Erqing Jin et al.
Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering. Along the way, combinatorial gene expressio...
Engineering of Yarrowia lipolytica for production of astaxanthin [0.03%]
利用Yarrowia lipolytica细胞工厂高效合成虾青素
Kanchana Rueksomtawin Kildegaard,Belén Adiego-Pérez,David Doménech Belda et al.
Kanchana Rueksomtawin Kildegaard et al.
Astaxanthin is a red-colored carotenoid, used as food and feed additive. Astaxanthin is mainly produced by chemical synthesis, however, the process is expensive and synthetic astaxanthin is not approved for human consumption. In this study,...
A systems approach using OSMAC, Log P and NMR fingerprinting: An approach to novelty [0.03%]
基于OSMAC、LogP和NMR指纹的系统方法：一种新颖性研究的方法论
Miaomiao Liu,Tanja Grkovic,Xueting Liu et al.
Miaomiao Liu et al.
The growing number of sequenced microbial genomes has revealed a remarkably large number of secondary metabolite biosynthetic clusters for which the compounds are still unknown. The aim of the present work was to apply a strategy to detect ...
Arieh Zaritsky,Avinoam Rabinovitch,Chenli Liu et al.
Arieh Zaritsky et al.
Cell size of bacteria M is related to 3 temporal parameters: chromosome replication time C, period from replication-termination to subsequent division D, and doubling time τ. Steady-state, bacillary cells grow exponentially by extending le...
Production of anthocyanins in metabolically engineered microorganisms: Current status and perspectives [0.03%]
代谢工程微生物中花色苷的生产：现状与展望
Jian Zha,Mattheos A G Koffas
Jian Zha
Microbial production of plant-derived natural products by engineered microorganisms has achieved great success thanks to large extend to metabolic engineering and synthetic biology. Anthocyanins, the water-soluble colored pigments found in ...