Comparative functional genomics of the acarbose producers reveals potential targets for metabolic engineering [0.03%]
类黄杆菌属青霉素产生菌的功能基因组比较分析揭示了代谢工程的潜在靶点
Huixin Xie,Qinqin Zhao,Xin Zhang et al.
Huixin Xie et al.
The α-glucosidase inhibitor acarbose is produced in large-scale by strains derived from Actinoplanes sp. SE50 and used widely for the treatment of type-2 diabetes. Compared with the wild-type SE50, a high-yield derivative Actinoplanes sp. ...
A LuxR family transcriptional regulator AniF promotes the production of anisomycin and its derivatives in Streptomyces hygrospinosus var. beijingensis [0.03%]
放线菌素D产生菌北京变种Streptomyces hygrospinosus var. beijingensis中一类LuxR型转录调控因子AniF促进放线菌素D及其衍生物生成
Jufang Shen,Lingxin Kong,Yan Li et al.
Jufang Shen et al.
The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits potent selective activity against pathogenic protozoa and fungi. It is one of the important effective components in Agricultural Antibiotic12...
Streptomyces avermitilis industrial strain as cell factory for Ivermectin B1a production [0.03%]
放线菌 avermirtinis 工业菌株作为伊维菌素B1a的细胞工厂
Qian Deng,Liqiongzi Xiao,Yang Liu et al.
Qian Deng et al.
Ivermectin, a kind of valuable derivatives of Avermectin, is distinct from Avermectin due to the saturated bond at C22-C23 position. Combinatorial biosynthesis of Ivermectins based on Avermectins biosynthetic gene cluster (ave) has been ach...
Engineering and modification of microbial chassis for systems and synthetic biology [0.03%]
工程改造微生物底盘细胞用于系统与合成生物学研究
Haotian Chi,Xiaoli Wang,Yue Shao et al.
Haotian Chi et al.
Engineering and modifying synthetic microbial chassis is one of the best ways not only to unravel the fundamental principles of life but also to enhance applications in the health, medicine, agricultural, veterinary, and food industries. Th...
Characterization of the positive SARP family regulator PieR for improving piericidin A1 production in Streptomyces piomogeues var. Hangzhouwanensis [0.03%]
表征 positively 表达的 SARP 家族调节因子 PieR 以提高杭州湾拟轮枝链霉菌中皮ericidin A1 的产量
Yan Li,Lingxin Kong,Jufang Shen et al.
Yan Li et al.
Piericidin A1, a member of ɑ-pyridone antibiotic, exhibits various biological activities such as antimicrobial, antifungal, and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitiv...
Crystal structure of the condensation domain from lovastatin polyketide synthase [0.03%]
洛伐他汀聚酮合酶凝缩结构域晶体结构
Lei Wang,Meijuan Yuan,Jianting Zheng
Lei Wang
The highly reducing iterative polyketide synthases responsible for lovastatin biosynthesis contains a section homologous to condensation (CON) domain observed in nonribosomal peptide synthetases (NRPSs). In the present study, we expressed t...
Rational and semi-rational engineering of cytochrome P450s for biotechnological applications [0.03%]
用于生物技术应用的细胞色素P450的理性及半理性工程改造
Lian-Hua Xu,Yi-Ling Du
Lian-Hua Xu
The cytochrome P450 enzymes are ubiquitous heme-thiolate proteins performing regioselective and stereoselective oxygenation reactions in cellular metabolism. Due to their broad substrate scope and catalytic versatility, P450 enzymes are als...
The functional differentiation of the post-PKS tailoring oxygenases contributed to the chemical diversities of atypical angucyclines [0.03%]
PKS后尾酶的功能分化有利于非典型安哥洛菌素类化合物的化学多样性
Keqiang Fan,Qian Zhang
Keqiang Fan
Angucyclines are one of the largest families of aromatic polyketides with various chemical structures and bioactivities. Decades of studies have made it easy for us to depict the picture of their early biosynthetic pathways. Two families of...
Systematically investigating the key features of the DNase deactivated Cpf1 for tunable transcription regulation in prokaryotic cells [0.03%]
系统研究原核细胞中可调转录调控的DNase失活Cpf1的关键特性
Chensi Miao,Huiwei Zhao,Long Qian et al.
Chensi Miao et al.
With a unique crRNA processing capability, the CRISPR associated Cpf1 protein holds great potential for multiplex gene regulation. Unlike the well-studied Cas9 protein, however, conversion of Cpf1 to a transcription regulator and its relate...
Genomics-guided discovery of a new and significantly better source of anticancer natural drug FK228 [0.03%]
基因组学引导发现抗癌天然药物FK228的新来源
Xiangyang Liu,Feng Xie,Leah B Doughty et al.
Xiangyang Liu et al.
FK228 is an FDA-approved anticancer drug naturally produced by Chromobacterium violaceum No. 968 up to 19 mg/L in a pilot industry-scale batch fermentation. Here we report a genomics-guided discovery of Burkholderia thailandensis MSMB43 as ...