Metabolic engineering of Corynebacterium glutamicum S9114 based on whole-genome sequencing for efficient N-acetylglucosamine synthesis [0.03%]
基于全基因组测序的大肠鸟氨酸菌株S9114的代谢工程研究及其高效N-乙酰氨基葡萄糖合成能力
Chen Deng,Xueqin Lv,Yanfeng Liu et al.
Chen Deng et al.
Glucosamine (GlcN) and its acetylated derivative N-acetylglucosamine (GlcNAc) are widely used in the pharmaceutical industries. Here, we attempted to achieve efficient production of GlcNAc via genomic engineering of Corynebacterium glutamic...
Genomic and transcriptional changes in response to pinene tolerance and overproduction in evolved Escherichia coli [0.03%]
适应松油烯抗性和过量产生的大肠杆菌基因组和转录变化
Fu-Xing Niu,Yuan-Bin Huang,Liang-Nian Ji et al.
Fu-Xing Niu et al.
α-Pinene is an important monoterpene, which is widely used as a flavoring agent and in fragrances, pharmaceuticals and biofuels. Although an evolved strain Escherichia coli YZFP, which had higher tolerance to pinene and titer, has been suc...
The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining [0.03%]
T4噬菌体DNA连接酶可介导细菌染色体DSB损伤的非同源末端连接修复反应
Tianyuan Su,Fapeng Liu,Yizhao Chang et al.
Tianyuan Su et al.
DNA double-strand breaks (DSBs) are one of the most lethal forms of DNA damage that is not efficiently repaired in prokaryotes. Certain microorganisms can handle chromosomal DSBs using the error-prone non-homologous end joining (NHEJ) syste...
Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli [0.03%]
大肠杆菌中调节基因表达的合成UP元素的设计与合成
Kristin V Presnell,Madeleine Flexer-Harrison,Hal S Alper
Kristin V Presnell
Metabolic engineering requires fine-tuned gene expression for most pathway optimization applications. To develop a suitable suite of promoters, traditional bacterial promoter engineering efforts have focused on modifications to the core reg...
Simultaneously down-regulation of multiplex branch pathways using CRISPRi and fermentation optimization for enhancing β-amyrin production in Saccharomyces cerevisiae [0.03%]
利用CRISPR干扰和发酵优化同时下调多分支途径以增强酿酒酵母中齐墩果酸的生成
Jiangping Ni,Genlin Zhang,Lei Qin et al.
Jiangping Ni et al.
The production of β-amyrin in Saccharomyces cerevisiae is still low due to the inability of effectively regulating the endogenous metabolic pathway for competitive synthesis of β-amyrin precursors. In this study, we focused on two branche...
Resistance mechanisms and reprogramming of microorganisms for efficient biorefinery under multiple environmental stresses [0.03%]
应对多重环境压力的微生物抗性机制及其重编程以实现高效生物精炼
Ke Xu,Yun Seo Lee,Jun Li et al.
Ke Xu et al.
In the fermentation process of biorefinery, industrial strains are normally subjected to adverse environmental stresses, which leads to their slow growth, yield decline, a substantial increase in energy consumption, and other negative conse...
Efficient editing DNA regions with high sequence identity in actinomycetal genomes by a CRISPR-Cas9 system [0.03%]
利用CRISPR-Cas9系统高效编辑放线菌基因组中高同源序列区域
Jingjun Mo,Shuwen Wang,Wan Zhang et al.
Jingjun Mo et al.
Actinobacteria able to produce varieties of bioactive natural products have been long appreciated by the field of drug discovery and development. Recently, a few of CRISPR/Cas9 systems bearing different types of replicons (pSG5 and pIJ101) ...
CRISPR-PIN: Modifying gene position in the nucleus via dCas9-mediated tethering [0.03%]
通过dCas9介导的锚定改变基因在细胞核中的位置
Jyun-Liang Lin,Holly Ekas,Matthew Deaner et al.
Jyun-Liang Lin et al.
Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair, genetic recombination, and gene expression. Here, we present CRISPR-PIN, a CRISPR/dCas9-based tool that allows control of gene Position ...
Synergistic drug combinations prediction by integrating pharmacological data [0.03%]
通过整合药理数据预测药物组合的协同作用
Chengzhi Zhang,Guiying Yan
Chengzhi Zhang
There is compelling evidence that synergistic drug combinations have become promising strategies for combating complex diseases, and they have evident predominance comparing to traditional one drug - one disease approaches. In this paper, w...
Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation [0.03%]
利用多因素优化对下一代测序高通量质粒DNA文库构建过程的微型化研究
Lorna Suckling,Ciaran McFarlane,Chelsea Sawyer et al.
Lorna Suckling et al.
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plas...