OmniEdit: A unified CRISPR/Cas9 platform for precise genome engineering and strain optimization of the Cordyceps militaris cell factory [0.03%]
兼性编辑：一种统一的CRISPR / Cas9平台，用于精确的基因组工程和 Cordyceps militaris 细胞工厂菌株优化
Mengqian Liu,Sijin Chen,Jingting Wang et al.
Mengqian Liu et al.
Cordyceps militaris serves as a critical microbial cell factory for high-value bioactive compounds; however, the scarcity of versatile and sophisticated genome-editing toolsets significantly restricts its systematic metabolic engineering. T...
Xiaoru Zuo,Huizhi Zhang,Zhanwei Zhang et al.
Xiaoru Zuo et al.
The cyclic monoterpene p-menthane-3,8-diol exhibits highly effective mosquito-repellent activity. Current industrial production of PMD depends on chemically synthesized citronellal as a precursor. To provide a green, sustainable alternative...
The RNAi machinery regulates lovastatin biosynthesis via microRNA-like RNAs in industrial Aspergillus terreus [0.03%]
核糖核酸干扰机器通过微小RN A_like核糖核酸调节工业丛梗丝霉菌洛瓦他汀生物合成
Meng Gu,Dandan Feng,Yongjuan Liu et al.
Meng Gu et al.
The RNA interference (RNAi) machinery regulates diverse cellular processes in fungi, but its functions in industrial Aspergillus terreus remain unknown. Here, we systematically investigated two industrial strains: the itaconic acid-producin...
Efficient whole-cell biocatalytic synthesis of 2'-deoxy-2'-fluoroadenosine, a key building block for nucleic acid drugs [0.03%]
高效全细胞生物催化合成核苷酸药物关键骨架2'-氟-2'-脱氧腺苷
Dandan Feng,Tianshu Zhang,Guan Zhou et al.
Dandan Feng et al.
2'-Deoxy-2'-fluoroadenosine (2'-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2'-deoxy-2'-fluorouridine via one-pot transglycosylation catalyzed by a thymidin...
Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production in Serratia marcescens [0.03%]
工程改造枯草色杆菌S-(+)-香芹酮产生途径以节约ATP及提高生产效率
Yue Chen,Linbo Gou,Di Liu et al.
Yue Chen et al.
(S)-(+)-linalool, a valuable acyclic monoterpenol secondary metabolite of plants, finds extensive applications in the food, flavor and fragrance, pharmaceutical, and daily chemical industries. Microbial synthesis offers two pathways for its...
Precise l-threonine-to-l-isoleucine pathway regulation for engineering high-efficiency whole-cell biocatalysts [0.03%]
精准调控l-苏氨酸到l-异亮氨酸途径用于高效全细胞生物催化剂工程化改造
Heng Zhang,Fuqiang Song,Ke Wang et al.
Heng Zhang et al.
l-Isoleucine (L-Ile), a critical branched-chain amino acid with diverse applications in food, pharmaceutical, and cosmetic industries, is difficult to produce efficiently at scale in microbial systems due to metabolic bottlenecks and cofact...
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law [0.03%]
合成生物学国际治理中软法的功能与优化——从硬法的困境到软法的崛起
Yu Qin,Jiaxiang Hu,Kezhen Su
Yu Qin
Synthetic biology, as an emerging field that integrates life sciences and engineering technology, is driving profound transformations in global science, ethics, and legal systems. In international legal framework, the Biological Weapons Con...
Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii [0.03%]
转座子插入测序鉴定了参与Clostridium ljungdahlii产气发酵的新基因
Zhiyi Zhang,Fengjuan Yang,Yihan Liu et al.
Zhiyi Zhang et al.
The efficient utilization of industrial one-carbon (C1) gases, such as carbon dioxide (CO2) and carbon monoxide (CO), has attracted widespread interest in recent years. Clostridium ljungdahlii, a key chemoautotrophic bacterium, can ferment ...
The PreKit platform: Cryptic gene clusters activation and high-titer compound production [0.03%]
PreKit平台：隐秘基因簇激活及高滴度化合物产生
Zhongyu Chen,Yelin Duan,Lei Liu et al.
Zhongyu Chen et al.
The discovery of novel scaffold compounds or enzymes through genome mining of biosynthetic gene clusters (BGCs) represents cornerstone of drug development. A major obstacle, however, is that most microbial BGCs encoding secondary metabolite...
Remodeling memory T cells with chemotherapy and immune checkpoint inhibitors as host pre-conditioning to empower in vivo CAR-T therapy [0.03%]
化疗和免疫检查点抑制剂重塑宿主机体以活化体内CAR-T细胞治疗的记忆T细胞
Ding-Ya Sun,Jia-Lu Wang,Xin Li et al.
Ding-Ya Sun et al.
Chemotherapy and immune checkpoint inhibitors (ICIs) are widely utilized in cancer treatment, exerting not only direct cytotoxic effects on tumor cells but also significantly reshaping the systemic immune status of patients, particularly th...