Formate-driven efficient pyruvate biosynthesis in Vibrio natriegens [0.03%]
利用Vibrio natriegens高效合成丙酮酸的甲酸盐驱动途径
Wangshuying Deng,Xinyu Zhang,Jiayin Liu et al.
Wangshuying Deng et al.
Formic acid (FA), a key one-carbon liquid compound derived directly from CO2, can serve as a dual-purpose substrate in microbial metabolism, supplying both carbon and energy. Its potential for green biomanufacturing is immense, yet its inhe...
Time-resolved transcriptomics reveals mechanisms of acute and sustained low-pH adaptation in Corynebacterium glutamicum [0.03%]
时间分辨转录组学揭示了谷氨酸棒杆菌急性低pH适应和持续低pH适应的机制
Yuqing Hao,Jianyu Xu,Bo Sun et al.
Yuqing Hao et al.
Corynebacterium glutamicum is a major industrial cell factory for amino acid production. Acidic byproduct accumulation can lower broth pH, disrupt cytoplasmic pH homeostasis, induce oxidative stress, and ultimately compromise productivity. ...
Systematic mining and engineering of signal peptides to achieve efficient secretion of cephalosporin C acylase in Escherichia coli [0.03%]
系统挖掘和工程改造信号肽以实现头孢菌素C酰基酶在大肠杆菌中的高效分泌
Xinyi Ren,Zhiying Yang,Siyu Chen et al.
Xinyi Ren et al.
Cephalosporin C acylases (CCAs) catalyze the hydrolysis of cephalosporin C to 7-aminocephalosporanic acid, a key intermediate for semisynthetic cephalosporin antibiotics. The functional secretion of heterologous CCAs in Escherichia coli is ...
S267P mutation of OxyR regulator in Zymomonas mobilis: mechanism of oxidative stress tolerance and applications in cellulosic hydrolysate fermentation and oxidative stress monitoring [0.03%]
Zymomonas mobilis中OxyR调控因子的S267P突变：耐氧化胁迫机制及其在纤维素水解物发酵和抗氧化剂监控中的应用
Yi Wang,Binan Geng,Shuyi Liu et al.
Yi Wang et al.
The peroxide-sensing transcriptional regulator OxyR plays a pivotal role in bacterial oxidative stress responses. The OxyRS267P mutation was previously identified in Zymomonas mobilis ZMNP, a mutant strain with enhanced hydrolysate toleranc...
Advances in vehicles for in situ delivery: From classical vectors to biologically inspired structures [0.03%]
从经典载体到生物启发结构的原位递送载具进展
Hengyi Wang,Xiaoyan Tang,Xinyao Pan et al.
Hengyi Wang et al.
The emerging fields of synthetic biology and gene therapy rely on delivery systems to introduce the nucleic acids and proteins into recipient cells. Hence, the development of delivery tools with high specificity, strong manufacturability, a...
Heterologous expression of hagfish thread proteins EsTKα and EsTKγ in Pichia pastoris and in vitro self-assembly into an ordered fibrous network [0.03%]
表达于毕赤酵母中的免疫无脊椎动物蛋白EsTKα和EsTKγ的异源表达及体外自组装形成有序纤维网状结构研究
Zhiwen Lin,Yuxuan Sun,Hao Shi et al.
Zhiwen Lin et al.
Eptatretus stoutii thread keratin α and γ (EsTKα and EsTKγ) are essential structural components conferring hagfish slime with exceptional strength and toughness. These proteins exhibit exceptional mechanical properties, rendering them p...
Valorization of xylose mother liquor into salidroside by engineered Kluyveromyces marxianus [0.03%]
工程化酿酒酵母将木糖母液增值转化为山柳苷玛
Lingya Wang,Dongmei Wang,Jiong Hong
Lingya Wang
Large amounts of xylose mother liquor (XML) are produced as a byproduct of the xylose industry and are often treated as waste. Salidroside is a high-value glycoside with pharmaceutical and nutraceutical applications, but conventional extrac...
XRE-type transcriptional regulator ProR controls prodigiosin synthesis in Serratia marcescens JNB5-1 [0.03%]
Serratia marcescens JNB5-1中XRE型转录调节因子ProR调控硫赤酮生物合成
Mi Tang,Yanan Hao,Yixuan Zhao et al.
Mi Tang et al.
The gram-negative bacterium Serratia marcescens produces various secondary metabolites, including prodigiosin, althiomycin, and serratamolide. Among these, prodigiosin exhibits multiple biological activities such as antibacterial, anticance...
Enhancing cytidine biosynthesis by coupling minicell-forming morphology with biofilm-based fermentation [0.03%]
黏菌素生物合成耦合微细胞形态与生物被膜发酵增强工程细菌细胞工厂性能
Di Zhang,Changle Shao,Dong Liu et al.
Di Zhang et al.
Cytidine is an important nucleoside in the pharmaceutical and biotechnology fields. To improve its biosynthesis efficiency, this study proposes a strategy that integrates minicell-forming recombinant strains with biofilm-based fermentation....
Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach [0.03%]
基于多模式深度学习方法的融合蛋白肽连接器的功能定制
Zhong Li,Jiaxi Lu,Jingsong Cui et al.
Zhong Li et al.
Peptide linkers are critical modulators of function in fusion proteins, a foundational technology in modern bioengineering. However, the rational customization of linkers for specific applications remains challenging, hindered by an insuffi...