Facile production of d-arabinitol from waste cells via artificial in vitro enzymatic cascade [0.03%]
基于细胞工厂的L-阿拉伯糖醇生产及其代谢机制研究
Xueli Zhang,Zihan Cheng,Yilin Luan et al.
Xueli Zhang et al.
d-Arabinitol is a high-value platform chemical widely applied in food, cosmetic, and pharmaceutical industries, yet conventional production suffers from low conversion efficiency, prolonged cycles, and considerable environmental hazards. He...
Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses [0.03%]
基于RT-RAA-CRISPR/Cas12a的多联可视化POCT平台的开发及临床验证用于检测猪腹泻病毒
Ying Chen,Meijun Yu,Jinghan Lin et al.
Ying Chen et al.
Porcine epidemic diarrhea virus (PEDV), porcine rotavirus A (PoRVA), and porcine sapelovirus (PSV) are major causative agents of severe diarrhea in swine and frequently occur as co-infections, leading to significant financial losses. To add...
The acetylation mechanism and engineering strategy of isocitrate lyase for optimizing biosynthesis in Escherichia coli [0.03%]
异柠檬酸裂解酶乙酰化机制及工程策略在大肠杆菌中优化生物合成中的应用研究
Ruoyu Jia,Jichao Wang,Ying Zhang et al.
Ruoyu Jia et al.
Lysine acetylation is a widespread post-translational modification that fine-tunes cell physiology and metabolism. Yet its application in metabolic engineering remains underexplored. Here, we report that the key enzyme of the glyoxylate shu...
Eukaryotic cell-free protein synthesis: Chassis diversification, system engineering, and emerging applications [0.03%]
真核无细胞蛋白质合成：载体多样化、系统工程及其新兴应用
Shengxin Tong,Yiming Wang,Xin Wang et al.
Shengxin Tong et al.
The eukaryotic cell-free protein synthesis (CFPS) system, endowed with intrinsic post-translational modification capabilities and a complex molecular chaperone network, efficiently synthesizes functional proteins with correct conformation a...
Defense-associated reverse transcriptases define an emerging class of noncanonical DNA-writing systems [0.03%]
一类新型非经典DNA书写系统的防御相关逆转录酶的发现
Yaojun Tong
Yaojun Tong
Defense-associated reverse transcriptases (DRTs) are emerging as a mechanistically diverse class of bacterial antiviral enzymes. Rather than acting simply as RNA-to-DNA copying proteins, they generate a wide range of unconventional DNA prod...
Unconditional de novo generation and functional validation of synthetic promoters with a diffusion model [0.03%]
基于扩散模型的合成启动子的无条件从头生成及功能验证
Chen Lin,Zhengwen Zhu,Xinglong Wang et al.
Chen Lin et al.
Generative neural networks enable de novo design of synthetic genetic elements. Here, we present DiPro, an unconditional diffusion model that learns E. coli promoter features to generate novel sequences without conditional input. DiPro dire...
In situ continuous evolution of native transporter HGT1 unlocks xylose utilization in Kluyveromyces lactis [0.03%]
原位连续进化天然转运蛋白HGT1使乳酒球酵母利用木糖成为可能
Xiangdi Chen,Shuaili Chen,Jingwen Zhou et al.
Xiangdi Chen et al.
Efficient xylose valorization is a prerequisite for the industrial application of K luyveromyces lactis in lignocellulosic biorefining, yet the inefficiency of transmembrane transport remains a significant bottleneck. To address this, a com...
Heterologous expression of hydroxylase coupled with pathway optimization enables highly efficient ʟ-DOPA production in Corynebacterium glutamicum [0.03%]
异源羟基酶的表达与途径优化使棒状杆菌中L-多巴高效合成成为可能
Mengzhen Jia,Ran Duan,Yan Jiang et al.
Mengzhen Jia et al.
ʟ-3,4-dihydroxyphenylalanine (ʟ-DOPA), the direct precursor of the neurotransmitter dopamine, is a cornerstone therapeutic agent for Parkinson's disease. In this study, Corynebacterium glutamicum was used as a microbial chassis to systema...
Hfq-dependent artificial regulatory small RNAs for targeted gene expression control in the nitrogen-fixing Pseudomonas stutzeri A1501 [0.03%]
依赖Hfq的合成调节小RNA在固氮菌施氏假单胞菌A1501中用于靶向基因表达控制
Yueyue Han,Yuhan Zhang,Chao Li et al.
Yueyue Han et al.
Artificial trans-encoded small RNAs (atsRNAs), which repress translation by base pairing with target mRNAs, have proven to be powerful tools for regulation of gene expression for reconstructing cell factories and genetic circuits. Currently...
Development of an efficient genome editing system using recombinase-mediated cassette exchange in E. coli Nissle 1917 for large gene cluster integration and heterologous astaxanthin production [0.03%]
利用大肠杆菌尼氏1917菌株中的重组酶介导的盒式交换开发高效的基因组编辑系统进行大规模基因簇整合和异源虾青素生产
Chanjuan Jiang,Ruofei Guo,Hongluan Sun et al.
Chanjuan Jiang et al.
Escherichia coli Nissle 1917 (EcN) is a promising chassis strain in synthetic biology, but its application is limited by inefficient genetic manipulation. This study established an efficient genetic manipulation system for EcN via electropo...