Engineering of fructose-6-phosphate aldolase for one-carbon conversion to mannitol in a designed biotransformation system [0.03%]
工程设计果糖-6-磷酸醛缩酶用于一碳转化合成山梨醇的人工代谢途径研究
Dandan Wang,Qianzhen Dong,Peng Chen et al.
Dandan Wang et al.
Developing artificial synthetic pathways for converting one-carbon compounds into value-added chemicals represents a promising strategy for carbon-neutral manufacturing. Enzymatic C-C bond formation plays a central role in carbon-chain exte...
The direct conversion of strictosamide to pumiloside in planta expands the camptothecin biosynthetic pathway [0.03%]
从植物中直接转化(strictosamide到pumiloside)扩展了camptothecin生物合成路径
Yongpeng Li,Zhihan Wu,Junyu Chen et al.
Yongpeng Li et al.
Cancer is a globally devastating disease that severely threatens human health and impedes social development. Camptothecin and its derivatives serve as crucial chemotherapeutic agents in clinical cancer treatment, owing to their unique inhi...
Engineered bacteria in disease diagnosis and therapy: A synthetic biology perspective [0.03%]
合成生物学视角下的工程细菌在疾病诊疗中的应用
Yan Shen,Si-Ming Lu,Long Yang et al.
Yan Shen et al.
Synthetic biology is an interdisciplinary field that integrates knowledge and techniques from modern biology and many other disciplines to design and construct novel biological systems or to modify existing life forms. Its core technologies...
Systems engineering of Escherichia coli for high-level hydroxytyrosol production [0.03%]
大肠杆菌系统工程高效生产羟基酪醇
Jiaojiao Zuo,Shaolun Zhang,Wenxiao Huang et al.
Jiaojiao Zuo et al.
Hydroxytyrosol (HT) is a potent polyphenolic antioxidant widely utilized in the biomedical and food industries. However, its high-level microbial biosynthesis is primarily hindered by the metabolic flux imbalances and severe cellular toxici...
Profiling heterologous 3' terminator-dependent expression variation in Aspergillus oryzae [0.03%]
异源多核菌丝真菌黑曲霉中不同3'终止子的功能分析及应用研究
Chenxi Zhang,Ridwan Elemosho,Elena Sofia Brancato et al.
Chenxi Zhang et al.
Transcriptional terminators are key to defining transcript boundaries, ensuring mRNA maturation, and maintaining expression stability, yet they remain underexplored in filamentous fungi. Here, we systematically benchmarked 15 heterologous 3...
Transposon-based genome editing of industrial microorganisms: advances, challenges, and prospects [0.03%]
工业微生物的转座子基因组编辑：进展、挑战和前景
Zhiyi Zhang,Zheng Huang,Yilin Wang et al.
Zhiyi Zhang et al.
As mobile genetic elements, transposons play a crucial role in the adaptive evolution and genome engineering of industrial microorganisms. Their applications range from high-throughput functional genomics, enabling systematic genotype-pheno...
Directed evolution of heme biosynthetic enzymes enabled by a growth-coupled heme biosensor in Corynebacterium glutamicum [0.03%]
谷氨酸棒杆菌中外源型血红素生物传感器的建立及其在血红素生物合成酶定向进化中的应用
Fangming Mou,Dong Li,Hong Wang et al.
Fangming Mou et al.
Heme is an essential cofactor involved in diverse cellular processes and is an important target for microbial biosynthesis. However, engineering microbial heme production remains challenging due to the requirement for coordinated activity o...
Engineering the probiotic Escherichia coli Nissle 1917 as a host organism for coliphage production [0.03%]
工程改造常用于生产大肠杆菌噬菌体的宿主菌——益生菌大肠杆菌Nissle 1917
Hailin Zhang,Ru Zhu,Ruoting He et al.
Hailin Zhang et al.
Bacteriophage therapy emerges as a pivotal alternative in the ongoing battle against antibiotic-resistant bacteria. Employing probiotic bacteria as hosts for bacteriophage production bestows several notable advantages compared to traditiona...
Efficient PE system PE7-scFv-MLH1dn by optimizing the configuration of La and MLH1dn [0.03%]
通过调控La和MLH1dn的组成优化高效PE系统PE7-scFv-MLH1dn
Xuesong Li,Chaojun Qing,Bo Li et al.
Xuesong Li et al.
Prime editing is a versatile gene editing technology that has undergone significant enhancements since its inception. Currently, a recently reported prime editing system, PE7 combined with MLH1dn, incorporates two auxiliary components desig...
Enhanced production of l-histidine in Escherichia coli through systematic metabolic engineering and CER controlled fermentation [0.03%]
基于系统代谢工程和CER控制发酵的L-组氨酸产量增强研究
Bo Zhang,Xintian Liu,Tianjun Zhao et al.
Bo Zhang et al.
l-Histidine is an essential amino acid with important applications in pharmaceuticals and nutrition, highlighting the demand for efficient microbial production platforms. This study developed a high-performance Escherichia coli cell factory...