Two-step bioconversion of pyridoxal 5'-phosphate from pyridoxine through cofactor regeneration and pyridoxine 5'-phosphate oxidase evolution [0.03%]
一步再生辅因子和吡哆醇-5-磷酸氧化酶定向进化催化的高效合成维生素B6异构体新方法
Xinwei Yang,Beisi Lu,Meiling Wang et al.
Xinwei Yang et al.
Pyridoxal 5'-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains a significant challenge. In this study...
Synergistic engineering of monoterpenoid synthase and metabolic pathways to enhance α-terpineol production in Saccharomyces cerevisiae [0.03%]
协同工程化单萜合成酶和代谢途径以增强酿酒酵母中α-萜品醇的生成
Shuai Wang,Xinxin Wang,Ying Wang et al.
Shuai Wang et al.
Plant-derived monoterpenoid α-terpineol has gained significant attention due to its versatile applications in the food, cosmetic and pharmaceutical industries. Microbial biosynthesis offers an environmentally sustainable approach for its c...
iFLinkC-EZ: A scalable and automatable method for the assembly of complex fusion proteins and multi-gene expression constructs based on the iFLinkC framework [0.03%]
基于iFLinkC框架的复杂融合蛋白和多基因表达载体的可扩展及自动装配方法-iFLinkC-EZ
Philipp Kemp,Melinda Blat Belmonte,Max Schmidt et al.
Philipp Kemp et al.
Standardized methods for the assembly of DNA constructs have become indispensable in synthetic biology, biotechnology and basic research. The majority of frameworks-most prominently based on Golden Gate-have been developed and optimized for...
Engineering a heme-dependent tryptophan hydroxylase pathway in E. coli for enhanced melatonin production [0.03%]
一种用于增强褪黑素生产的基于血红素的色氨酸脱羧酶途径工程菌株(E.coli)
Luyao Zhang,Guobin Yin,Senyu Pan et al.
Luyao Zhang et al.
Melatonin is a high-value bioactive indoleamine broadly applied in the pharmaceutical, food and nutraceutical industries, yet its microbial production remains constrained by pathway complexity and low enzymatic efficiency. Here, a streamlin...
Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis [0.03%]
西安明霉素负调控转录因子的鉴定及功能研究
Wen-Yan He,Jia-Xin Li,Xu-Liang Bu et al.
Wen-Yan He et al.
Xiamenmycin, a prenylated benzopyran compound produced by Streptomyces xiamenensis 318, exhibits promising anti-fibrotic activity but suffers from low productivity, limiting its pharmaceutical development. In this study, we obtained a mutan...
Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization [0.03%]
高效全细胞生物转化法合成石蒜苷：糖基转移酶改造及工艺优化获得高产量石蒜苷
Xun Wang,Jiale Zhang,Tao Li et al.
Xun Wang et al.
Gastrodin is a naturally occurring phenolic glycoside derived from Gastrodia elata, which is widely used as an edible medicinal plant in functional foods and dietary supplements in East Asia. Owing to its antioxidant, neuroprotective, and m...
Development of mycelium-reinforced dialdehyde cellulose-polyvinyl alcohol composites for emerging high-performance sustainable materials [0.03%]
用于新兴高性能可持续材料的菌丝增强多醛纤维素-聚乙烯醇复合材料的发展
Pattaravaree Petchsong,Nattawut Boonyuen,Papichaya Kwantong et al.
Pattaravaree Petchsong et al.
Dialdehyde cellulose (DAC), featuring reactive carbonyl groups introduced via oxidation of hemp-derived cellulose fibers (confirmed by FTIR spectroscopy), was synthesized to promote strong hydrogen bonding with polyvinyl alcohol (PVA) for c...
Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic de novo DNA synthesis [0.03%]
末端脱氧核糖核酸转移酶的半理性工程改造用于高效从头合成DNA
Chengjie Zhang,Zhicong He,Zhengquan Gao et al.
Chengjie Zhang et al.
The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations, including short DNA elongation length...
Corrigendum to "Metabolic modulation of TCA cycle by S-nitrosylation in Monascus spp." [Synthetic and Systems Biotechnol 12 (2026) 490-501] [0.03%]
关于“S-亚硝基化调节红曲霉TCA循环代谢的研究”的勘误(合成与系统生物技术，12(2026):490-501)
Zitong Meng,Yingao Tan,Yali Duan et al.
Zitong Meng et al.
[This corrects the article DOI: 10.1016/j.synbio.2026.01.002.]. © 2026 The Authors.
Signal peptide engineering of a novel M4 family keratinase and the action mechanism in promoting pepper tolerance to salt stress through KerJY23-hydrolyzed feather waste [0.03%]
新型M4家族角蛋白酶信号肽改造及其通过KerJY23降解羽毛促进辣椒耐盐性的机制研究
Chao Duan,Yuanxing Wang,Tao Xiong et al.
Chao Duan et al.
Microbial keratinase has attracted significant attention for its ability to convert keratin waste into high-value products with substantial application potential in the agricultural field. However, the low yield and potential pathogenicity ...